Abstract
We have investigated H2 production on glucose, xylose, arabinose, and glycerol in Thermotoga maritima and T. neapolitana. Both species metabolised all sugars with hydrogen yields of 2.7–3.8 mol mol−1 sugar. Both pentoses were at least comparable to glucose with respect to their qualities as substrates for hydrogen production, while glycerol was not metabolised by either species. Glycerol was also not metabolised by T. elfii. We also demonstrated that T. neapolitana can use wet oxidised wheat straws, in which most sugars are stored in glycoside polymers, for growth and efficient hydrogen production, while glucose, xylose and arabinose are consumed in parallel.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Introduction
Biohydrogen can be produced from organic materials by a range of different bacteria. Hydrogen production processes have been characterised in complex as well as in defined growth media. When glucose is used as substrate, the yield of hydrogen has for many bacterial cultures been in the range of 1–2.5 mol H2 mol−1 glucose (Rachman et al. 1998; Mizuno et al. 2000; Fabiano and Perego 2002; Oh et al. 2003; Lin and Chang 2004; Morimoto et al. 2004; Kotay and Das 2007). In several hyperthermophilic bacteria belonging to the genus Thermotoga, yields of hydrogen on glucose is higher and may approach the theoretical maximum yield of 4 mol H2 mol−1 glucose. Schröder et al. (1994) reported that Thermotoga maritima converted 1 mol glucose into 2 mol acetic acid and 4 mol of H2. Takahata et al. (2001) found yields of 3.7 and 4 mol H2 mol−1 glucose in T. petrophila and T. naphtophila, respectively, while van Niel et al. (2002) found a yield of 3.8 mol H2 mol−1 glucose in T. elfii. In T. neapolitana, which has been extensively characterised with respect to hydrogen production, van Ooteghem et al. (2002, 2004) reported H2 yields at or even above 4 mol H2 mol−1 glucose, Eriksen et al. (2008) found 3.5–3.8 mol H2 mol−1 glucose when no lactic acid was formed, Munro et al. (2009) found 3.85 mol H2 mol−1 glucose at the optimal temperature of 77°C, and d’Ippolito et al. (2010) found 4.05 mol H2 mol−1 glucose, and that 12–15% of the hydrogen was produced from yeast extract added to the growth medium. Also in other hyperthermophilic bacteria, hydrogen yields on glucose may approach the theoretical maximum (Verhaart et al. 2010).
The high yields of hydrogen in Thermotoga seem related to a bifurcating hydrogenase that has recently been characterised in T. maritima (Schut and Adams 2009). This hydrogenase needs NADH as well as reduced ferredoxin in a 1:2 stoichiometric ratio in order to function and is unable to use neither NADH nor reduced ferredoxin as the sole substrate. While the reduction potential of NADH is insufficient to effectively reduce protons to hydrogen, bacteria harbouring the bifurcating hydrogenase can use excess reducing power from reduced ferredoxin, which is a stronger reducing agent, to enhance the efficiency of proton reduction by NADH. When sugars are oxidised via glycolysis and the pentose phosphate pathway, NADH and reduced ferredoxin are produced exactly in a 1:2 stoichiometric ratio, and all reducing equivalents from sugar oxidation can therefore be used to produce hydrogen.
Most studies on hydrogen production in Thermotoga have used glucose as carbon source although hydrogen productions at a large scale will have to be based on cheaper substrates, such as plant biomass or waste streams like for example mash from the fermentation industry. These materials often have high contents of pentoses stored in carbohydrate polymers. Members of Thermotoga can grow on pentoses and encode and secrete amylases, cellulases, xylanases, pectinases and other glycoside hydrolases (Nelson et al. 1999; Chhabra et al. 2003; Conners et al. 2006). The theoretical maximal yield of hydrogen on pentoses is 3.3 mol mol−1, but whether this yield can be obtained in Thermotoga cultures is not known.
Considerable amounts of glycerol are also available as waste from the biodiesel industry and in mash from fermentation industries where it is produced by Saccharomyces cerevisiae. The genome of T. maritima contains coding sequences for a complete pathway for uptake and conversion of glycerol (Nelson et al. 1999) and van Ooteghem et al. (2004) found a positive signal for oxidation of glycerol by T. neapolitana in a microplate assay. A single report also describes hydrogen production in T. neapolitana on waste glycerol, although the yield of hydrogen as well as the degree of glycerol conversion was low (Ngo et al. 2009). It is therefore not clear how well Thermotoga is able to utilise glycerol for growth and hydrogen synthesis.
In this study we have quantified yields of hydrogen in T. maritima and T. neapolitana growing on xylose or arabinose, the two dominating pentoses in angiosperms, as well as glucose and glycerol, and demonstrated that hydrogen production in Thermotoga can be carried out with high yields also on complex carbohydrates.
Materials and methods
Strain and growth media
Thermotoga maritima DSM 3109, T. neapolitana DSM 4359, and T. elfii DSM 9442 were grown in a modified version of the ATCC 1977 medium (see van Ooteghem et al. 2002) containing 1 g cysteine·HCl l−1 as reducing agent, and glucose, xylose, arabinose or glycerol as carbon substrate. The medium also contained 2 g yeast extract l−1 and 2 g peptone l−1, which are needed by these bacteria. Potassium phosphate was increased from 0.6 to 6.3 g l−1 to increase the buffering capacity of the growth medium (Eriksen et al. 2008), while the concentrations of MgCl·H2O and CaCl2·H2O were reduced to 0.2 and 0.01 g l−1, respectively, to prevent formation of precipitates. MgCl, CaCl2 and the carbon source (glucose, xylose, arabinose, or glycerol) were autoclaved in a separate solution before mixed with a solution containing the remaining medium constituents. A few crystals of resazurin were added as redox indicator.
T. neapolitana was also grown on media based on wheat straw, which had been pre-treated by wet-oxidation at 195°C, under 12 bar O2 pressure, and with 2 g Na2CO3 l−1 added for 15 min (Varga et al. 2002). Remaining solids were removed by centrifugation, while 2 g peptone l−1 was added.
Culture conditions
Batch cultures of T. maritima, T. neapolitana, and T. elfii were grown in 120 ml serum bottles containing 60 ml medium with shaking at 200 rpm and 80°C. Before the cultures were inoculated, the bottles were sealed by butyl rubber stoppers and flushed with N2.
Cultures of T. neapolitana were also grown in a 3 l Applikon bioreactor containing 2.75 l medium. The reactor was equipped with a Pt100 temperature sensor and an autoclavable pH electrode (Mettler Toledo). Temperature and pH were automatically controlled by a heating blanket wrapped around the reactor wall, and by titration with 1 M NaOH. Cultures were stirred by a 4 bladed Rushton turbine and aerated by headspace or by sparger aeration with nitrogen gas. The exit gas was passed through a condenser at 4°C to reduce evaporation before it was passed through on-line sensors for quantification of gas-flow rate (AWM3000, Honeywell), and hydrogen (EZT3HYE ‘Easy Cal’ CiTiceL) and CO2 (IRcel, City Technology, Portsmouth, UK) in the off-gas.
Sampling and analyses
Gas and liquid samples from the head-spaces of the serum bottles were taken using syringes fitted with needles. Excess gas pressure in a serum bottle was released into a syringe and the amount of gas, which had been present in the headspace, was estimated at ambient air pressure as the total volume of gas in the flask and in the syringe. H2 and CO2 in the headspace gas were measured by GC.: 0.3 ml headspace gas equilibrated to ambient temperature was injected at 110°C, and separated on a 2 m Hyacep Q column at 80°C. The gasses were quantified by a thermal conductivity detector against known standards. N2 was used as carrier at 10.3 ml min−1.
Growth was determined from the OD800 values, and correlated to the dry weight which was determined after filtration onto pre-dried 0.2 μm disc filters and drying at 105°C, and by microscopic observation.
Glucose, xylose, arabinose, glycerol and organic acid concentrations as well as Maillard products formed in culture supernatants were quantified using HPLC: 50 μl of 0.22 μm filtered culture supernatant was separated on an Aminex HPX-87H column (Bio-rad), eluted with 0.5 mM H2SO4 at 0.4 ml min−1 and 30°C. Detection was performed with a refractive index detector. Total concentrations of glucose, xylose, arabinose in media based on wet oxidised wheat straw were measured after acid hydrolysis in 4% (w/v) H2SO4, for 10 min at 121°C.
Results and discussion
Hydrogen production on glucose, xylose and arabinose
All 3 Thermotoga species investigated: T. maritima, T. neapolitana and T. elfii grew and produced hydrogen on xylose, arabinose, as well as on glucose, which was included as substrate to allow comparisons of results to previous studies. Figure 1a shows, as an example, the consumption of arabinose and the production of hydrogen, CO2, acetic acid and lactic acid in a 60 ml T. maritima batch culture grown in a closed serum bottle.
The stoichiometry of glucose, xylose, or arabinose conversion was quantified by plotting the produced amounts of the various metabolic products against the amount of sugar consumed (Fig. 1b). Table 1 summarises the observed molar yields of hydrogen on glucose, xylose and arabinose in T. maritima and in T. neapolitana. Two processes may have affected the yields shown in Table 1: metabolization of components from yeast extract and peptone by the cells, and by chemical processes transforming part of the sugars into Maillard products (Fig. 1a). d’Ippolito et al. (2010) found that approx. 12–15% of the hydrogen produced in T. neapolitana came from conversion of such substrates, resulting in overestimation of the hydrogen yield on sugar. However, the amounts of sugar that went into Maillard reactions, predominantly in early stages of the cultures, were also in the order of 10–15%. Since the Maillard products, which were detectable by HPLC, were partly re-metabolised in late stages of batch cultures, the Maillard reactions have probably effected the estimated hydrogen yields to a lesser extend than the up-take of additional substrates, and the estimated hydrogen yields on sugars are therefore slightly higher than the true yields.
The molar of yield of hydrogen on glucose was 3–4 in both species and similar to previously reported yields on glucose (Schröder et al. 1994; van Ooteghem et al. 2002, 2004; Eriksen et al. 2008; Munro et al. 2009; d’Ippolito et al. 2010). This indicates that our cultures have been grown at conditions that are comparable to conditions generally used for hydrogen production studies in Thermotoga.
The molar yields of hydrogen on xylose and arabinose were also close to or above 3. In T. neapolitana, the measured hydrogen yields were even above the theoretical maximal value of 3.3 mol hydrogen per mol pentose (Table 1), most likely because yeast extract and peptone have played greater roles in as substrates in T. neapolitana cultures compared to T. maritima cultures. Carbon balances, which included the sugar used as substrates, and the produced CO2, acetic acid, and lactic acid were positive in T. neapolitana cultures, while slightly negative in T. maritima cultures. Biomass was not included in the carbon balances since the biomass is predominantly produced from components in the yeast extract and peptone.
The rate by which hydrogen was produced was highest in T. neapolitana cultures (Table 1). During the growth phase, the average volumetric hydrogen production rates were 2–3 times higher in T. neapolitana than in T. maritima cultures, and similar in magnitude to the hydrogen production rates of 0.8–0.9 mmol l−1 h−1 previously found in T. neapolitana under similar conditions (Munro et al. 2009). Also the specific hydrogen production rates, estimated as the yield of hydrogen on biomass (Fig. 1b, inset) during initial phases when growth was exponential multiplied by the specific growth rate, were highest in T. neapolitana. Individual cells were most productive in early stages of batch cultures but gradually their activity decreased. In some cultures, activity stopped before all sugar was taken up, a phenomenon that has been explained by decreasing pH (Eriksen et al. 2008; Munro et al. 2009) but also metabolites, CO2 and hydrogen accumulated and may have repressed the cultures. We found no systematic differences in hydrogen production rates on pentoses compared to glucose. The hydrogen yields and production rates shown in Table 1 therefore show that xylose and arabinose are at least comparable to glucose with respects to their qualities as substrates for hydrogen production in Thermotoga.
Glycerol as substrate for hydrogen production
Glycerol would also be an interesting substrate for hydrogen production in Thermotoga. However, we found no conversion of glycerol in cultures of T. maritima, T. neapolitana, nor T. elfii (data not shown). If glycerol was supplemented as sole carbon source, none of the 3 species grew and they did not produce more hydrogen than cultures supplemented only with yeast extract and peptone. If glycerol was supplemented simultaneously with one or more sugar species, all species took up the sugars but left the glycerol untouched.
Glycerol is apparently not a suitable substrate for hydrogen production in Thermotoga, possibly as a consequence of the bifurcating hydrogenase present in these bacteria. While hexoses or pentoses are metabolised with a concurrent production of NADH and reduced ferredoxin in the 1:2 stoichiometric ratio needed by the hydrogenase (Schut and Adams 2009), conversion of glycerol leads to formation of 2 mol NADH and 2 mol reduced ferredoxin per mol glycerol. Reduction of acetic acid to ethanol would be a potential route for re-oxidation of excess NADH. The T. maritima genome encodes a putative alcohol dehydrogenase (Nelson et al. 1999) and trace amounts of ethanol have been reported in cultures of T. hypogea (Fardeau et al. 1997), but ethanol was not detected in our cultures. These bacteria may therefore need access to alternative electron acceptors, like elemental sulphur (Huber et al. 1986) that can be reduced directly by NADH in order to use glycerol as substrate, but this will decrease the yield of hydrogen.
Hydrogen production on biomass based substrates
T. neapolitana grew and produced hydrogen on the liquid fraction generated from wet oxidation of wheat straw. The culture, shown in Fig. 2, was grown in a bioreactor where pH was controlled and hydrogen and CO2 were continuously removed, in order to minimise inhibition of the process and allow maximal conversion of carbohydrates. The liquid fraction used as medium contained a mixture of free xylose, glucose and arabinose (7 mM) and the same sugars bound in glycoside polymers, probably mainly hemicellulose, corresponding to a monomer concentration of 24 mM. Also 15 mM acetic acid was present after the wet oxidation.
After a lag of 1 day, the culture started to produce H2 and convert the sugars while biomass concentrations increased to reach a maximum of 0.5 g l−1 after 2.5 days (Fig. 2a). The apparent decrease in biomass concentration after 2.5 days was at least partly associated to biofilm formation, which developed at this point and brought a fraction of the cells out of suspension. Glucose, xylose, and arabinose were taken up in parallel, as also observed in cultures grown on two or more sugars in serum bottles. de Vrije et al. (2009) also observed parallel consumption of glucose and xylose in T. neapolitana and a second hyperthermophilic hydrogen producer, Caldicellulosiruptor saccharolyticus grown on Miscanthus hydrolysates. Most of the carbohydrates used by the culture in Fig. 2 were initially found in carbohydrate polymers, as hydrolysis was not included in the pre-treatment of the wheat straws. Glucose has previously been shown to repress β-galactosidase activity and lactose metabolism in T. neapolitana resulting in diauxic growth (Vargas and Noll 1996), and glucose represses the synthesis of glycoside hydrolases at the transcription level in T. maritima (Chhabra et al. 2003). Still, the majority of the carbohydrate polymers were degraded in periods where the total sugar monomer concentration was in the order of 5 mM (Fig. 2b). Starch in untreated potato steam peels is also a suitable substrate for hydrogen synthesis in T. neapolitana (Mars et al. 2010). Catabolite repression by glucose or other sugars seems therefore not to play a decisive role in the ability of T. neapolitana to simultaneously degrade and utilise a variety of polymeric substrates, something that is quit advantageously in relation to the employment of these bacteria in hydrogen production processes.
The culture shown in Fig. 2 consumed a total of 27 mM sugars with a corresponding hydrogen production of 72.5 mmol l−1, and a hydrogen yield on sugar of 2.6 mol mol−1, or 74% of the theoretical maximal value based on the relative amounts of glucose and pentoses consumed. In 9 additional T. neapolitana batch or fed-batch cultures grown in the bioreactor on defined media with glucose as carbon source, the hydrogen yield on glucose was 3.8 ± 0.2 mol mol−1 (data not shown). This corresponds to 95% of the maximum theoretical yield, and is similar to what was found in cultures grown in serum bottles (Table 1). The slightly lower molar yield of hydrogen in the culture in Fig. 2 can have been affected by the presence of alternative electron acceptors or inhibitors in the wet oxidised materials but the yield was still above what is found in mesophilic bacteria (Rachman et al. 1998; Mizuno et al. 2000; Fabiano and Perego 2002; Oh et al. 2003; Lin and Chang 2004; Morimoto et al. 2004; Kotay and Das 2007), even though culture conditions and composition of biomass based media have not yet been optimised.
Hydrogen production potentials
Large amounts of organic feed-stocks will be needed if H2 is to replace a considerable fraction of the hydrogen that is currently produced from fossil fuels and amounted more than 20 billion kg in the US in year 2008 (Bromaghim et al. 2010) and 3 times more world wide (van Ooteghem et al. 2002). In Table 2 we have, as an example, estimated how much of this current hydrogen production that in 2030 potentially can be replaced by bio-H2 produced from leftover mash from the 2. generation industry. The US Department of Energy (2008) predicts a growth in bioethanol production from close to zero in 2010 to 6 × 1010 kg by 2030 in the US or 1.2 × 1011 kg world wide. The mash therefore represents a resource not yet utilised by other applications. Depending on feed-stock, and provided that pentose fermentation is not developed, this annual mash production will contain in the order of 2.8 × 1011 or 5.6 × 1011 mol pentoses in the US or world wide, respectively. From these pentoses, a maximum of 9 × 1011 or 1.8 × 1012 mol hydrogen can be produced to replace 9% of the current hydrogen production in the US or 6% world wide. If the hydrogen yield on pentoses is reduced to 75%, as was the case in the culture shown in Fig. 2, mash from bioethanol production will be able to provide enough feedstock to cover only 7 or 4.5% of the annual hydrogen production the US or world wide, respectively (Table 2). These numbers illustrate the massive scale by which large scale bio-H2 needs to be produced, and the enormous challenge it will be to secure sufficient biomass resources for this purpose. This is why biological hydrogen producers should be able to utilise a broad spectrum of substrates and produce hydrogen with yields close to the theoretical maximum on hexoses as well as on pentoses. Several Thermotoga species fulfil both criteria, and are at the moment among the most promising candidates for employment in biohydrogen production processes.
References
Bromaghim G, Gibeault K, Serfass J, Serfass P, Wagner E (2010) Hydrogen and fuel cells: the U.S. market report. A report by the National Hydrogen Association on 2008 data
Chhabra SR, Shockley KR, Conners SB, Scott KL, Wolfinger RD, Kelly RM (2003) Carbohydrate-induced differential gene expression patterns in the hyperthermophilic bacterium Thermotoga maritima. J Biol Chem 278:7540–7552
Conners SB, Mongodin EF, Johnson MR, Montero CI, Nelson KE, Kelly RM (2006) Microbial biochemistry, physiology, and biotechnology of hyperthermophilic Thermotoga species. FEMS Microbiol Rev 30:872–905
d’Ippolito G, Dipasquala L, Vella FM, Romano I, Gambacorta A, Fontana A (2010) Hydrogen metabolism in the extreme thermophile Thermotoga neapolitana. Int J Hydrog Energy 35:2290–2295
de Vrije T, Bakker RR, Budde MAW, Lai MH, Mars AE, Claassen PAM (2009) Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana. Biotechnol Biofuels 2:12
Eriksen NT, Nielsen TM, Iversen N (2008) Hydrogen production in anaerobic and microaerobic Thermotoga neapolitana. Biotechnol Lett 30:103–109
Fabiano B, Perego P (2002) Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes. Int J Hydrog Energy 27:149–156
Fardeau M-L, Ollivier B, Patel BK, Magot M, Thomas P, Rimbault A, Rocchiccioli F, Garcia J-L (1997) Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019
Hamelinck CN, van Hooijdonk G, Faaij APC (2005) Ethanol from lignocellulosic biomass: techno-economic performance in short-, middle- and long-term. Biomass Bioenergy 28:384–410
Huber R, Langworthy TA, König H, Thomm M, Woese CR, Sleytr YB, Stetter KO (1986) Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C. Arch Microbiol 144:324–333
Kotay SM, Das D (2007) Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge. Bioresour Technol 98:1183–1190
Lin CY, Chang R-C (2004) Fermentative hydrogen production at ambient temperature. Int J Hydrog Energy 29:715–720
Mars AE, Veuskens T, Budde MAW, van Doeveren PFNM, Lips SJ, Bakker RR, de Vrije T, Claassen PAM (2010) Biohydrogen production from untreated and hydrolyzed potato steam peels by the extreme thermophiles Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana. Int J Hydrog Energy 35:7730–7737
Mizuno O, Dinsdale R, Hawkes FR, Hawkes DL, Noike T (2000) Enhancement of hydrogen production from glucose by nitrogen gas sparging. Bioresour Technol 73:59–65
Morimoto M, Atsuko M, Atif AAY, Ngan MA, Fakhru’l-Razi A, Iyuke SE, Bakir AM (2004) Biological production of hydrogen from glucose by natural anaerobic microflora. Int J Hydrog Energy 29:709–713
Munro SA, Zinder SH, Walker LP (2009) The fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production. Biotechnol Prog 25:1035–1042
Nelson KE, Clayton RA, Gill SR, Gwinn ML, Dodson RJ, Haft DH, Hickey EK, Peterson JD, Nelson WE, Ketchum KA, McDonald L, Utterback TR, Malek JA, Linher KD, Garett MM, Stewart AM, Cotton MD, Pratt MS, Phillips CA, Richardson D, Heidelberg J, Sutton GG, Fleischmann RD, Eisen JA, White O, Salzberg SL, Smith HO, Venter JC, Fraser CM (1999) Evidence for lateral gene transfer between archae and bacteria from genome sequence of Thermotoga maritima. Nature 399:323–329
Ngo TA, Kim K-R, Nguyen T-AD, Kim M-S, Sim S-J (2009) Fermentative hydrogen production from glycerol wastes of biodiesel manufacture by Thermotoga neapolitana. Proceedings of the 3rd international conference on fermentation technology for value added agricultural products, Khon Kaen, Thailand
Oh Y-K, Seol E-H, Kim JR, Park S (2003) Fermentative biohydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19. Int J Hydrog Energy 28:1353–1359
Rachman MA, Nakashimada Y, Kakizono T, Nishio N (1998) Hydrogen production with high yield and high evolution rate by self-flocculated cells of Enterobacter aerogenes in a packed-bed reactor. Appl Microbiol Biotechnol 49:450–454
Schröder C, Selig M, Schönheit P (1994) Glucose fermentation to acetate, CO2 and H2 in the anaerobic hyperthermophilic eubacterium Thermotoga maritima; involvement of the Embden-Meyerhof pathway. Arch Microbiol 161:460–470
Schut GJ, Adams MWW (2009) The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production. J Bacteriol 191:4451–4457
Takahata Y, Nishijima M, Hoaki T, Maruyama T (2001) Thermotoga petrophila sp. nov. and Thermotoga naphtophila sp. nov., two hyperthermophilic bacteria from the Kubiki oil reservoir in Niigata, Japan. Int J Syst Evol Microbiol 51:1901–1909
U.S. Department of Energy (2008) World biofuels production potential. Understanding the challenges to meeting the U.S. renewable fuel standard, 67 pp
van Niel EWJ, Budde MAW, de Haas GG, van der Wal FJ, Claassen PAM, Stams AJM (2002) Distinctive properties of high hydrogen producing extreme thermophiles, Caldicellusiruptor saccharolyticus and Thermotoga elfii. Int J Hydrog Energy 27:1391–1398
Van Ooteghem SA, Beer SK, Yue PC (2002) Hydrogen production by the thermophilic bacterium Thermotoga neapolitana. Appl Biochem Biotechnol 98–100:177–189
Van Ooteghem SA, Jones A, van der Lelie D, Dong B, Mahajan D (2004) H2 production and carbon utilization by Thermotoga neapolitana under anaerobic and microaerobic growth conditions. Biotechnol Lett 26:1223–1232
Varga E, Szengyel Z, Reczey K (2002) Chemical pretreatments of corn stover for enhancing enzymatic digestibility. Appl Biochem Biotechnol 98–100:73–87
Vargas M, Noll KM (1996) Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is independent of cAMP. Microbiology 142:139–144
Verhaart MRA, Bielen AAM, van der Oost J, Stams AJM, Kengen SWM (2010) Hydrogen production by hyperthermophilic and extremely thermophilic bacteria and archaea: mechanisms for reductant disposal. Environ Technol 31:993–1003
Acknowledgments
We thank Anne Belinda Thomsen, Risø DTU for supplying wet oxidised wheat straw.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Eriksen, N.T., Riis, M.L., Holm, N.K. et al. H2 synthesis from pentoses and biomass in Thermotoga spp.. Biotechnol Lett 33, 293–300 (2011). https://doi.org/10.1007/s10529-010-0439-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-010-0439-x