Abstract
A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted β-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.
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Acknowledgements
We gratefully acknowledge the financial support of the National New Productions Project from the Science and Technology Ministry (P. R. China), and we thank the Bacillus Genetic Stock Centre of Ohio State University for generously providing the study materials.
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Ming-Ming, Y., Wei-Wei, Z., Xi-Feng, Z. et al. Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis . Biotechnol Lett 28, 1713–1718 (2006). https://doi.org/10.1007/s10529-006-9146-z
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DOI: https://doi.org/10.1007/s10529-006-9146-z