Abstract
A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) has been isolated from Zea mays by screening a cDNA library. The cDNA, designated ZmPLC, encodes a polypeptide of 586 amino acids, containing the catalytic X, Y and C2 domains found in all PI-PLCs from plants. Northern blot analysis showed that the expression of the ZmPLC gene in roots is up-regulated under conditions of high salt, dehydration, cold or low osmotic stress conditions. Recombinant ZmPLC protein was expressed in Esch- erichia coli, purified and used to produce polyclonal antibody, this polyclonal antibody is important for further studies to assess the ultimate function of the ZmPLC gene in plants.
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Zhai, S., Sui, Z., Yang, A. et al. Characterization of a Novel Phosphoinositide-Specific Phospholipase C from Zea mays and its Expression in Escherichia coli. Biotechnol Lett 27, 799–804 (2005). https://doi.org/10.1007/s10529-005-5802-y
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DOI: https://doi.org/10.1007/s10529-005-5802-y