Abstract
A peptide fusion to the capsid protein (CP) of Cucumber mosaic virus(CMV) was designed to express either a 17 amino acid (aa) neutralizing epitope of the Newcastle disease virus (NDV) fusion (F) protein or an eight aa neutralizing epitope of the NDV hemagglutinin-neuraminidase (HN) protein. Fusions of the F,␣HN and HN2 (duplicated HN epitope) were made in the internal βH-βI loop (motif 5) within the CMV CP. Recombinant RNA3 transcripts of the Ixora strain of CMV were inoculated on to Nicotiana benthamiana, together with CMV RNA1 and CMV RNA2. When the F and HN epitopes were placed in the internal motif, the modified virus was infectious and the HN NDV epitope was recognized by anti-NDV sera. However, in some plants, deletions of one to several of the inserted amino acids occurred. A duplication of the HN epitope rendered the virus non-viable.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
DJ Alexander (Eds) (1988) Newcastle Disease. Kluwer Academic Publishers Boston
M Bendahamane M Koo E Karrer RN Beachy (1999) ArticleTitleDisplay of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions J. Mol. Biol. 290 9–20
P Chambers M Nesbit K Yusoff NS Millar AC Samson PT Emmerson (1988) ArticleTitleLocation of a neutralizing epitope for the haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus. J. Gen.Virol. 69 2115–2122 Occurrence Handle1:CAS:528:DyaL1cXlsVOmt7g%3D
X He S Liu KL Perry (1998) ArticleTitleIdentification of epitopes in cucumber mosaic virus using a phage-dispayed random peptide library. J. Gen Virol. 79 3145–3153 Occurrence Handle1:CAS:528:DyaK1cXotVaqu70%3D
S Liu X He G Park C Josegsson KL Perry (2002) ArticleTitleA conserved capsid protein surface domain of Cucumber mosaic virus is essential for efficient aphid vector transmission. J. Virol 76 9756–9762 Occurrence Handle1:CAS:528:DC%2BD38XntFGks7Y%3D
H Lot J Marrou JB Quiot CA Esvan (1972) ArticleTitleA contribution to the study on cucumber mosaic virus (CMV).. II. Quick method of purification Ann. Phytopath. 4 25–38 Occurrence Handle1:CAS:528:DyaE38XkvFylur4%3D
A Natilla G Piazolla M Nuzzaci P Saldarelli C Tortorella A Antonaci P Piazzolla (2004) ArticleTitleCucumber mosaic virus as carrier of a hepatitis C virus-derived epitope Arch Virol 149 137–154 Occurrence Handle1:CAS:528:DC%2BD3sXpvVKqtrk%3D
JCK Ng S Liu KL Perry (2000) ArticleTitleCucumber mosaic virus mutants with altered physical properties and defective in aphid vector transmission Virology 276 395–403 Occurrence Handle1:CAS:528:DC%2BD3cXntl2nurs%3D
P Palukaitis MJ Roossinck RG Dietzgen RIB Francki (1992) ArticleTitleCucumber mosaic virus. Adv.Virus Res. 41 281–348 Occurrence Handle10.1016/S0065-3527(08)60039-1 Occurrence Handle1:CAS:528:DyaK3sXjsVGn
GP Pogue GP Lomonossoff (1998) ArticleTitleScope for using plant viruses to present epitopes from animal pathogens. Rev Med. Virol. 8 25–41
GP Pogue JA Lindbo SJ Garger WP Fitzmaurice (2002) ArticleTitleMaking an ally from an enemy: plant virology and the new agriculture Annu. Rev. Phytopathol 40 45–74 Occurrence Handle1:CAS:528:DC%2BD38Xos1CltLg%3D
C Porta Spall VE KC Findlay RC Gergerich CE Farrance GP Lomonossoff (2003) ArticleTitleCowpea mosaic virus-based chimeras Effects of inserted peptides on the phenotype, host range, and transmissibility of the modified viruses. Virology 310 50–63 Occurrence Handle1:CAS:528:DC%2BD3sXkt1yjt70%3D
TJ Smith E Chase T Schmidt KL Perry (2000) ArticleTitleThe structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus J. Virol. 74 7578–7586 Occurrence Handle1:CAS:528:DC%2BD3cXlt1Grsb4%3D
SJ Streatfield JA Howard (2003) ArticleTitlePlant-based vaccines. Int J.Parasitol. 33 479–493 Occurrence Handle1:CAS:528:DC%2BD3sXktFCqtLk%3D
KM Taylor C Porta T Lin JE Johnson PJ Barker GP Lomonossoff (1999) ArticleTitlePosition-dependent processing of peptides presented on the surface of cowpea mosaic virus Biol. Chem. 380 387–392 Occurrence Handle1:CAS:528:DyaK1MXitlChur8%3D
ME Tousignant PM McGarvey P Piazzolla JM Kaper (1996) ArticleTitleCucumber mosaic virus from Ixora: infectious RNA transcripts confirm deficiency in satellite support and unusual symptomatology Res. Virol. 147 363–373 Occurrence Handle1:CAS:528:DyaK2sXislCgtQ%3D%3D
T Toyoda B Gotoh T Sakaguchi H Kida Y Nagai (1988) ArticleTitleIdentification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization. J. Virol 62 4427–4430 Occurrence Handle1:CAS:528:DyaL1MXksFyq
Author information
Authors and Affiliations
Corresponding author
Additional information
Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture.
Rights and permissions
About this article
Cite this article
Zhao, Y., Hammond, R.W. Development of a candidate vaccine for Newcastle disease virus by epitope display in the Cucumber mosaic virus capsid protein. Biotechnol Lett 27, 375–382 (2005). https://doi.org/10.1007/s10529-005-1773-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-1773-2