Abstract
The effect of (−)deprenyl (D) on prolonging survival has previously been reported in different species of animals. In rats, three studies reported a positive effect, while one study reported a shortening of life spans. In the present study, we attempted to clarify past discrepancies in the results based on the speculation that there exists a certain effective dose range for this effect of the drug. F344/DuCrj rats of both sexes began to receive subcutaneous (s.c.) injections of D at the age of 18 months at a dose of 0.25 mg/kg/injection (inj.), 3 times a week. Control animals were given a vehicle (a saline solution). Average life spans of animals (days) were significantly increased in both male (895 ± 109.7, n=30; 967.8 ± 88.6, n=30, control vs. D treated, P<0.01, t-test) and female (924.7 ± 132.2, n=38; 987.1 ± 133.4, n=39, P<0.05) rats by 8.1% and 6.7%, respectively. We have previously reported that a dose of 0.5mg/kg/inj. (s.c.) significantly increased the life span of male F344 rats, while a dose of 1.0 mg/kg/inj. somewhat shortened the life span, although the difference was not statistically significant. The results of the present study coupled with our previous reports clearly indicate that a proper dose of D within a certain dose range can significantly increase the life span of animals of both sexes, but that a greater dose becomes less effective and may actually adversely affect the life span of rats. The presence of this effective dose range of D may explain discrepancies in the effect of D on life spans of animals previously reported.
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Abbreviations
- CAT:
-
catalase
- D:
-
(-)depreny
- inj:
-
injection
- s.c.:
-
subcutaneous
- SOD:
-
superoxide dismutase
- TMIG:
-
Tokyo Metropolitan Institute of Gerontology
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Kitani, K., Kanai, S., Miyasaka, K. et al. Dose-dependency of Life Span Prolongation of F344/DuCrj Rats Injected with (−)deprenyl. Biogerontology 6, 297–302 (2005). https://doi.org/10.1007/s10522-005-4804-4
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DOI: https://doi.org/10.1007/s10522-005-4804-4