Abstract
We have used semi quantitative RT-PCR and western blotting for the analysis of expression of estrogen receptor (ER) α and βmRNA and protein in the kidney of adult and old mice of both sexes. Uterus, a well-known target for estrogen action, was used for comparison. As compared to adult, the expression of both ER α mRNA and protein of old mice decreased in male but increased in female. However, unlike uterus, neither ERβ mRNA nor protein could be detected in the kidney of adult or old mice. Thus the present data reveal that the expression of ERα in mice kidney shows sex differences during aging.
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Abbreviations
- βME:
-
βmercaptoethanol
- ECL:
-
enhanced chemiluminescence
- EDTA:
-
ethylenediaminetetraacetic acid
- ER:
-
Estrogen receptor
- PAGE:
-
polyacrylamide gel electrophoresis
- PBS:
-
phosphate buffered saline
- PCR:
-
polymerase chain reaction
- PMSF:
-
phenyl methyl sulphonyl fluoride
- PVDF:
-
polyvinyl difluoride
- RT:
-
reverse transcriptase
- SDS:
-
sodium dodecyl sulphate
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Sharma, P.K., Thakur, M.K. Estrogen receptor α expression in mice kidney shows sex differences during aging. Biogerontology 5, 375–381 (2004). https://doi.org/10.1007/s10522-004-3191-6
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DOI: https://doi.org/10.1007/s10522-004-3191-6