A gene-cell construct based on rat olfactory mucosa ensheathing cells transduced with an adenoviral vector encoding a mature form of brain neurotrophic factor (mBDNF) was transplanted into post-traumatic cysts of rat spinal cord. Transplantation of the gene-cell construct improved motor activity of the hind limbs and reduced the size of cysts in some animals. However, comparison of the effects of transduced and non-transduced ensheathing cells revealed no significant differences. In parallel in vitro experiments, a decrease in the proliferation of transduced cells compared to non-transduced cells was observed. It is likely that mBDNF reduces proliferation of transduced cells, which can affect their efficiency. The therapeutic efficacy of the new gene-cell construct is most likely provided by the cellular component.
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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 211-216, December, 2021
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Karsuntseva, E.K., Fursa, G.A., Sosnovtseva, A.O. et al. Application of a New Gene-Cell Construct Based on the Olfactory Mucosa Escheating Cells Transduced with an Adenoviral Vector Encoding Mature BDNF in the Therapy of Spinal Cord Cysts. Bull Exp Biol Med 172, 617–621 (2022). https://doi.org/10.1007/s10517-022-05446-x
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DOI: https://doi.org/10.1007/s10517-022-05446-x