We compared the state of actin cytoskeleton, morphology, and expression of VE-cadherin in endothelial cells of human umbilical cord vein under conditions of TNF-α-mediated activation and microgravity modeling and found that 3D-clinorotation for 24 h impaired the integrity of endothelial monolayer, altered cell morphology, induced cytoskeleton reorganization, and reduced the expression of VE-cadherin. The combination of experimental microgravity and proinflammatory activation led to more pronounced clearing of the perinuclear space from microfilaments and accumulation of depolymerized actin, which confi rms additive effect of the studied factors on actin cytoskeleton of endothelial cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
A. S. Antonov, A. V. Krushinskii, M. A. Nikolaeva, et al., Tsitologiya, 23, No. 10, 1154–1159 (1981).
L. B. Buravkova, Aviakosm. Ekol. Med., 42, No. 6, 10–18 (2008).
L. B. Buravkova and N. V. Merzlikina, Aviakosm. Ekol. Med., 38, No. 6, 56–61 (2004).
M. P. Gershovich, Yu. G. Gershovich, and L. B. Buravkova, Aviakosm. Ekol. Med., 45, No. 4, 39–41 (2011).
Yu. A. Romanov, N. V. Kabaeva, and L. B. Buravkova, Aviakosm. Ekol. Med., 34, No. 4, 23–26 (2000).
Yu. A. Romanov, N. V. Kabaeva, and L. B. Buravkova, Aviakosm. Ekol. Med., 35, No. 1, 37–40 (2001).
K. M. Baldwin, Med. Sci. Sports Exerc., 28, No. 8, 983–987 (1996).
G. Bazzoni and E. Dejana, Physiol. Rev., 84, No. 3, 869–901 (2004).
J. B. Boonyaratanakornkit, A. Cogoli, C. F. Li, et al., FASEB J., 19, No. 14, 2020–2022 (2005).
L. B. Buravkova and Y. A. Romanov, Acta Astronaut., 48, No. 5–12, 647–650 (2001).
S. B. Campos, S. L. Ashworth, S. Wean, et al., Am. J. Physiol. Renal. Physiol., 296, No. 3, F487-F495 (2009).
S. I. Carlsson, M. T. Bertilaccio, E. Ballabio, and J. A. Maier, Biochim. Biophys. Acta, 1642, No. 3, 173–179 (2003).
P. Carmeliet, M. G. Lampugnani, L. Moons, et al., Cell, 98, No. 2, 147–157 (1999).
S. J. Crawford-Young, Int. J. Dev. Biol., 50, Nos. 2–3, 183–191 (2006).
P. F. Davies and S. C. Tripathi, Circ. Res., 72, No. 2, 239–245 (1993).
J. M. Fritsch-Yelle, U. A. Leuenberger, D. S. D’Aunno, et al., Am. J. Cardiol., 81, No. 11, 1391–1392 (1998).
D. Grimm, J. Bauer, P. Cossmehl, et al., FASEB J., 16, No. 6, 604–606 (2002).
A. Hall, Science, 279, 509–514 (1998).
M. Imamizo-Sato, A. Higashibata, and N. Ishioka, Biol. Sci. Space, 16, No. 3, 203–204 (2002).
M. Infanger, P. Kossmehl, M. Shakibaei, et al., Apoptosis, 11, No. 5, 749–764 (2006).
B. D. Levine, J. H. Zuckerman, and J. A. Pawelczyk, Circulation, 96, No. 2, 517–525 (1997).
M. L. Lewis, L. A. Cubano, B. Zhao, et al., FASEB J., 15, No. 10, 1783–1785 (2001).
D. Li, B. Yang, and J. L. Mehta, Cardiovasc. Res., 42, No. 3, 805–813 (1999).
V. Mako, J. Czucz, Z. Weiszhar, et al., Cytometry, Part A, 77, No. 10, 962–970 (2010).
C. Michiels, J. Cell. Physiol., 196, No. 3, 430–443 (2003).
F. E. Nwariaku, Z. Liu, X. Zhu, et al., Blood, 104, No. 10, 3214–3220 (2004).
J. S. Pober, M. P. Bevilacqua, D. L. Mendrick, et al., J. Immunol., 136, No. 5, 1680–1687 (1986).
D. S. Sangha, S. Han, R. E. Purdy, J. Appl. Physiol., 91, No. 2, 789–796 (2001).
F. Shi, Y. C. Wang, T. Z. Zhao, et al., PLoS One, 7, No. 7, doi: 10.1371/journal.pone.0040365 (2012).
K. M. Stroka, J. A. Vaitkus, and H. Aranda-Espinoza, Eur. Biophys., 41, No. 11, 939–947 (2012).
M. Toborek, S. Barger, M.P. Mattson, et al., J. Lipid Res., 37, No. 1, 123–135 (1996).
E. Tzima, M. Irani-Tehrani, W. B. Kiosses, et al., Nature, 437, 426–431 (2005).
B. M. Uva, M. A. Masini, M. Sturla, et al., Brain Res., 934, No. 2, 132–139 (2002).
J. J. W. A. van Loon, J. P. Veldhuijzen, J. Kiss, et al., Adv. Space Res., 39, No. 7, 1161–1165 (2007).
J. Vassy, S. Portet, M. Beil, et al., FASEB J., 15, No. 6, 1104–1106 (2001).
P. A. Watson, FASEB J., 5, No. 7, 2013–2019 (1991).
W. I. Weis and W. J. Nelson, J. Biol. Chem., 281, No. 47, 35,593-35,597 (2006).
R. White and M. Averner, Nature, 409, 1115–1118 (2001).
B. Wojciak-Stothard, A. Entwistle, R. Garg, AND A. J. Ridley, J. Cell. Physiol., 176, No. 1, 150–165 (1998).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 260–267, October, 2014
Rights and permissions
About this article
Cite this article
Rudimov, E.G., Buravkov, S.V., Andreeva, E.P. et al. Effect of Proinflammatory Activation on F-Actin Distribution in Cultured Human Endothelial Cells under Conditions of Experimental Microgravity. Bull Exp Biol Med 158, 573–580 (2015). https://doi.org/10.1007/s10517-015-2809-9
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-015-2809-9