We developed an original method of isolation and analysis of cytogenetic micropreparations of mouse oocytes including treatment with buffered hypotonic saline, paraformaldehyde fixation, and fluorescent staining. The method had several advantages, including high quality of sections and low labour intensity.
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N. B. Rubtsov, Methods of Operations with Mammalian Chromosomes [in Russian], Novosibirsk (2006).
A. P. Dyban, Stain Technol., 58, No. 2, 69-72 (1983).
G. Ertizeid and R. Storeng, J. Reprod. Fertil., 96, No. 4, 649-655 (1992).
ESHRE Capri Workshop Group, Hum. Reprod. Update, 14, No. 4, 293-307 (2008).
Y. Kamiguchi, K. Funaki, and K. Mikamo, Proc. Jpn Acad., 52, 316-319 (1976).
N. S. Macklon, J. P. Geraedts, and B. C. Fauser, Hum. Reprod. Update, 8, No. 4, 333-343 (2002).
J. B. Mailhes and Z. P. Yuan, Gamete Res., 18, No. 1, 77-83 (1987).
Manipulating the Mouse Embryo: A Laboratory Manual, Eds. A. Nagy, et al., Cold Spring Harbor (2003), 148-149.
D. Moralli, M. Yusuf, M. A. Mandegar, et al., Stem Cell Rev., 7, No. 2, 471-477 (2011).
T. Raposa and A. T. Natarajan, Humangenetik, 21, No. 3, 221-226 (1974).
A. Russo, Mutat. Res., 455, Nos. 1-2, 167-189 (2000).
A. K. Tarkowski, Cytogenetics, 5, 394-400 (1966).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 156, No. 7, pp. 128-132, July, 2013
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Pligina, K.L., Zhanataev, A.K., Chaika, Z.V. et al. Method of Cytogenetic Assay of Mouse Oocytes. Bull Exp Biol Med 156, 114–118 (2013). https://doi.org/10.1007/s10517-013-2292-0
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DOI: https://doi.org/10.1007/s10517-013-2292-0