We developed a method of rapid assay for biochemical properties of the saliva based on attenuated total reflection infrared spectroscopy. This method allows evaluating saliva composition within 5 min without sample preparation; 10 μl sample is enough for the analysis. The concentration of total protein, glucose, secretory immunoglobulin A, urea, amylase, cortisol, inorganic phosphate in the saliva can also be measured. Precision and reproducibility of the evaluated parameters are comparable to those obtained by routine clinical analysis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
A. B. Denisov. Saliva and Salivary Glands [in Russian], Moscow (2009).
G. Antonelli, E. Cappellin, R. Gatti, et al., Clin. Biochem., 40, No. 8, 545–550 (2007).
L. Benezzeddine-Boussaidi, G. Cazorla, and A. M. Melin, Clin. Chem. Lab. Med., 47, No. 1, 83–90 (2009).
C. E. Christersson, L. Lindh, and T. Arnebrant, Eur. J. Oral. Sci., 108, No. 5, 418–425 (2000).
G. Hosafci, O. Klein, G. Oremek, and W. Mantele, Anal. Bioanal. Chem., 387, No. 5, 1815–1822 (2007).
D. Koh, V. Ng, L. H. Chua, et al., Occup. Environ. Med., 60, No. 9, 696–698 (2003).
J. G. Lewis, Clin. Biochem. Rev., 27, No. 3, 139–146 (2006).
M. Mulcahy, D. S. Evans, S. K. Hammond, et al., Tob. Control, 14 , No. 6, 384–388 (2005).
C. Rehbinder, J. Hau, Can. J. Vet. Res., 70, No. 2, 151–154 (2006).
R. V. Santos, A. L. Almeida, E. C. Caperuto, et al., Comp. Biochem. Physiol. B Biochem. Mol. Biol., 45, No. 1, 114–117 (2006).
C. P. Schultz, M. K. Ahmed, C. Dawes, and H. H. Mantsch, Anal. Biochem., 240, No. 1, 7–12 (1996).
D. Soo-Quee Koh and G. Choon-Huat Koh, Occup. Envir. Med., 64, No. 3, 202–210 (2007).
N. P. Walsh, A. K. Blannin, A. M. Clark, et al., J. Sports Sci, 17, No. 2, 129–134 (1999).
Y. P. Zhou, L. Xu, L. J. Tang, et al., Anal. Sci., 23, No. 7, 793–798 (2007).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 148, No. 11, pp. 597–600, November, 2009
Rights and permissions
About this article
Cite this article
Khaustova, S.A., Shkurnikov, M.U., Grebenyuk, E.S. et al. Assessment of Biochemical Characteristics of the Saliva Using Fourier Transform Mid-Infrared Spectroscopy. Bull Exp Biol Med 148, 841–844 (2009). https://doi.org/10.1007/s10517-010-0831-5
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-010-0831-5