The composition and kinetics of accumulation of extracellular DNA in cultures of primary human endotheliocytes, cervical adenocarcinoma, and mycoplasma-infected cervical adenocarcinoma cells were studied. The content of DNA bound to cell surface did not change during culturing. The concentration of extracellular DNA in culture medium increased during the lag phase and at the beginning of the exponential growth phase, which probably attests to active secretion of DNA by cells. Spontaneous extracellular DNA synthesis was observed only in cell culture infected with mycoplasma.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
P. Anker, M. Stroun, and P. A. Maurice, Cancer Res., 36, No. 8, 2832–2839 (1976).
M. F. Barile, Science, 105, 155–189 (1993).
O. Dussurget and D. Roulland-Dussoix, Appl. Environ. Microbiol., 60, No. 3, 953–959 (1994).
E. A. Jaffe, R. L. Nachman, C. G. Becker, and C. R. Minick, J. Clin. Invest., 52, No. 11, 2745–2756 (1973).
S. Jahr, H. Hentze, S. Englisch, et al., Cancer Res., No. 4, 1659–1665 (2001).
P. P. Laktionov, S. N. Tamkovich, E. Y. Rykova, et al., Ann. N. Y. Akad. Sci., 1022, 221–227 (2004).
V. S. Mal’shakova, FEBS J., 272, 442–443 (2005).
E. S. Morozkin, P. P. Laktionov, E. Y. Rykova, and V. V. Vlassov, Anal. Biochem., 322, No. 1, 48–50 (2003).
E. S. Morozkin, E. Y. Rykova, V. V. Vlassov, and P. P. Laktionov, Ann. N. Y. Akad. Sci., 1075, 341–346 (2006).
M. Stroun, P. Anker, P. A. Maurice, et al., Int. Rev. Cytol., 51, 1–48 (1977).
M. Stroun, P. A. Maurice, V. Vasioukhin, et al., Ann. N. Y. Akad. Sci., 906, 161–168 (2000).
M. Stroun, J. Lyautey, C. Lederrey, et al., Clin. Chim. Acta, 313, Nos. 1-2, 139–142 (2001).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 147, No. 1, pp. 67-70, January, 2009
Rights and permissions
About this article
Cite this article
Morozkin, E.S., Sil’nikov, V.N., Rykova, E.Y. et al. Extracellular DNA in Culture of Primary and Transformed Cells, Infected and Not Infected with Mycoplasma. Bull Exp Biol Med 147, 63–65 (2009). https://doi.org/10.1007/s10517-009-0448-8
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-009-0448-8