Abstract.
The stored-product mites are the most abundant and frequent group of pests living on the stored food products in Europe. They endanger public health since they produce allergens and transmit mycotoxin-producing fungi. Novel acaricidal compounds with inhibitory effects on the digestive enzymes of arthropods are a safe alternative to the traditional neurotoxic pesticides used for control of the stored-product pests. In this work, we explored the properties of acarbose, the low molecular weight inhibitor of α-amylases (AI), as a novel acaricide candidate for protection of the stored products from infestation by Acarus siro (Acari: Acaridae). In vitro analysis revealed that AI blocked efficiently the enzymatic activity of digestive amylases of A. siro, and decreased the physiological capacity of mite’s gut in utilizing a starch component of grain flour. In vivo experiments showed that AI suppressed the population growth of A. siro. The mites were kept for three weeks on experimental diet enriched by AI in concentration range of 0.005 to 0.25%. Population growth of A. siro was negatively correlated with the content of AI in the treated diet with a half-population dose of 0.125%. The suppressive effect of AIs on stored-product mites is discussed in the context of their potential application in GMO crops
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
I.A. Akimov V.V. Barabanova (1976) ArticleTitleThe digestive enzyme of acaroid mites Dopov. Akad. nauk. ukr. RSR. 6B 547–549
G. Alberti A. Seiczak S. Seniczak (2003) ArticleTitleThe digestive system and fat body of an early-derivative oribatid mite Archegozetes longisetosus Aoki (Acari: OribatidaTrhypochthoniidae) Acarologia 83 149–219
L.G. Arlian (2002) ArticleTitleArthropod allergens and human health Ann. Rev. Entomol. 47 395–434 Occurrence Handle1:CAS:528:DC%2BD38XnvVWmtQ%3D%3D
D.A. Cook D.M. Armitage (2000) ArticleTitleEfficacy of a diatomaceous earth against mite and insect populations in small bins of wheat under conditions of low temperature and high humidity Pest Manag. Sci. 56 591–596 Occurrence Handle1:CAS:528:DC%2BD3cXks1yntLo%3D
C.E. Bowman (1984) Comparative enzymology of economically important astigmatid mites D.A. Griffiths C.E. Bowman (Eds) Acarology 6. Vol. 2 Ellis Horwood ChichesterNew York 993–1001
C.E. Bowman M. Childs (1982) ArticleTitlePolysaccharides in astigmatid mites (Arthropoda: Acari) Comp. Biochem. Physiol. 72 551–557
M.J. Chrispeels (1998) ArticleTitleGenetic engineering with α-amylase inhibitors makes seeds resistant to bruchids Seed Sci. Res. 8 257–263 Occurrence Handle10.1017/S0960258500004153 Occurrence Handle1:CAS:528:DyaK1cXkvVektL8%3D
J.T. Franz G. Masuch H. Musken K.C. Bergmann (1997) ArticleTitleMite fauna of German farms Allergy 52 1233–1237 Occurrence Handle9450144 Occurrence Handle1:STN:280:DyaK1c7htFOqtQ%3D%3D
M.R. Franzolin W. Gambale R.G. Cuero B. Correa (1999) ArticleTitleInteraction between toxigenic Apergillus flavus Link and mites (Tyrophagus putrescentiae Schrank) on maize grains: effects on fungal growth and aflatoxin production J. Stored Prod. Res. 35 215–224
A.M.R. Gatehouse K.A. Fenton I. Jepson D.J. Pavey (1986) ArticleTitleThe effects of α-amylase in vitro and effects on development in vivo J. Sci. Food Agric. 37 727–734 Occurrence Handle1:CAS:528:DyaL28XlsFWkur8%3D
A.M.R. Gatehouse J.A. Gatehouse (1998) ArticleTitleIdentifying proteins with insecticidal activity: use of encoding genes to produce insect-resistant transgenic crops Pest. Sci. 52 165–175 Occurrence Handle1:CAS:528:DyaK1cXhtlajsrY%3D
C. Gilles J.P. Astier G. Marchis-Mouren C. Cambillau F. Payan (1996) ArticleTitleCrystal structure of pig pancreatic alpha-amylase isoenzyme II, in complex with the carbohydrate inhibitor acarbose Eur. J. Biochem. 238 561–569 Occurrence Handle8681972 Occurrence Handle1:CAS:528:DyaK28XjsVKksrs%3D
X. Hou P.G. Fields (2003) ArticleTitleGranary trial of protein enriched pea flour for the control of three stored-product insect in barley J. Econ. Entomol. 96 1005–1015 Occurrence Handle12852648 Occurrence Handle10.1603/0022-0493-96.3.1005
J. Hubert V. Šustr (2001) ArticleTitleThe effect of starvation on microanatomical and physiological features of Galumna elimata (Acari: Oribatida) Eur. J. Entomol 98 265–275
Hubert J., Šobotník J., Mourek J. and Žd’ árková E. 2002a. The anatomy and ultrastructure of the digestive tract of storage mite Acarus siro (Acari: Acaridida). Abstract presented in 7th European Congress of Entomology, ThessalonikiGreece 333pp.
Hubert J., Mareš M., Dolečková L., Pravcová M., Kluh I., Jarošík V., Kocourek F., Křeček P. and Stejskal V. 2002b. The effect of amylase inhibitor on the development of red flour beetle Tribolium castaneum. Abstract presented in 7th European Congress of Entomology, ThessalonikiGreece 319pp.
J. Hubert V. Stejskal A. Kubátová Z. Munzbergová M. Váňová E. Žd’ árková (2003) ArticleTitleMites as selective fungal carriers in stored grain habitats Exp. Appl. Acarol. 29 69–87 Occurrence Handle14580060
J. Hubert V. Jarosik J. Mourek A. Kubatova E. Zdarkova (2004) ArticleTitleAstigmatid mite growth and fungi preference (Acari: Acaridida): comparisons in laboratory experiments Pedobiologia 48 205–214
M. Iversen J. Korsgaard T. Hallas R. Dahl (1990) ArticleTitleMite allergy and exposure to storage mites and house dust mites in farmers Cli. Exp. Allergy 20 211–219 Occurrence Handle1:STN:280:DyaK3c3ptFaqtA%3D%3D
E.H. Kim H.K. Kim D.H. Choi Y.J. Ahn (2003) ArticleTitleAcaricidal activity of clove bud oil compounds against Tyrophagus putrescentiae (Acari: Acaridae) Appl. Entomol. Zool. 38 261–266 Occurrence Handle1:CAS:528:DC%2BD3sXpt1Omurk%3D
J.J. Pueyo T.D. Morgan N. Ameenuddin C. Liang G.R. Reeck M.J. Chrispeels K. Kramer (1995) ArticleTitleEffect of bean and wheat inhibitors on α-amylase activity and growth of stored product insect pests Entomol. Exp. Appl. 75 237–244 Occurrence Handle1:CAS:528:DyaK2MXnt1CgtLw%3D
R. Sánchez-Monge G. García-Casado D. Barber G. Salcedo (1996) ArticleTitleInteraction of allergens from house-dust mite and from cereal flours: Dermatopagoides pteronyssinus α-amylase (Der p 4) and wheat and rye α-amylase inhibitors Allergy 51 176–180 Occurrence Handle8781672
T.H. Schuler G.M. Poppy B.R. Kerry I. Denholm (1999) ArticleTitlePotential side effects of insect-resistant transgenic plants on arthropod natural enemies TIBTECH 17 210–216 Occurrence Handle1:CAS:528:DyaK1MXlt1Kjsbk%3D
R.N. Sinha (1979) Role of Acarina in the stored grain ecosystem J.G. Rodriguez (Eds) Recent Advances in Acarology, Vol. 1 Academic Press London Ltd 263–272
J. Smrž (1989) ArticleTitleInternal anatomy of Hypochthonius rufulus (Acari: Oribatida) J. Morphol. 200 215–230
J. Smrž V. Čatská (1989) ArticleTitleThe effect of the consumption of some soil fungi on the internal microanatomy of the mite Tyrophagus putrescentiae (Schrank) (Acari: Acaridida) Acta Universitatis Carolinae-Biologica 33 81–93
V. Stejskal J. Hubert Z. Kučerová Z. Munzbergová J. Lukáš E. Žd’ árková (2003) ArticleTitleThe influence of the type of storage on pest infestation of stored grain in the Czech Republic Plant Soil Environ. 49 49–54
B.B. Thind J.P. Edwards (1990) ArticleTitleStimulation of egg production in the grain mite Acarus siro by the juvenile-hormone analogue fenoxycarb Exp. Appl. Acarol. 9 1–10 Occurrence Handle1:CAS:528:DyaK3MXltVyq
M. Van Hage-Hamsten S.G.O. Johansson (1992) ArticleTitleStorage mites Exp. Appl. Acarol. 16 117–128 Occurrence Handle1493741 Occurrence Handle1:STN:280:DyaK3s7lsVWrtQ%3D%3D
E. Žd’ árková (1998) ArticleTitleBiological control of storage mites by Cheyletus eruditus Integ. Pest Man. Rev. 3 111–116
D.R. Wilkin J.A. Hope (1973) ArticleTitleEvaluation of pesticides against stored product mites J. Stor. Prod. Res. 8 323–327 Occurrence Handle1:CAS:528:DyaE3sXlt1amsb0%3D
J.P. Woodring E.F. Cook (1962) ArticleTitleThe biology of Ceratozetes cisalpinus BerleseScheloribates laevigatus Kochand Oppia neerlandica Oudemans (Oribatei) with a description of all stages Acarologia 4 101–137
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hubert, J., Dolečková-Marešová, L., Hýblová, J. et al. In vitro and in vivo inhibition of α-amylases of stored-product mite Acarus siro . Exp Appl Acarol 35, 281–291 (2005). https://doi.org/10.1007/s10493-004-7834-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10493-004-7834-8