Abstract
A novel bacterium, strain b6T (T=type strain), was isolated from a disused mine site by growth using arsenite [As(III)] as energy source in a simple mineral medium. Cells of strain b6T were rod-shaped, Gram-negative, non-sporulating and motile. Optimum growth occurred at temperatures between 20 and 30 °C, and at pH between 4.0 and 7.5. Strain b6T grew chemoautotrophically on As(III), sulphur and thiosulphate, and also heterotrophically on yeast extract and a variety of defined organic compounds. Several other Thiomonas strains, including the type species Thiomonas (Tm.) intermedia, were able to oxidize As(III), though only strain b6T and strain NO115 could grow using As(III) as sole energy source in the absence of any organic compound. The G+C content of the DNA of strain b6T was 65.1 mol %. Comparative small subunit (SSU) ribosomal RNA (rRNA) analysis indicated that strain b6T belongs to the genus Thiomonas in the β-subdivision of the Proteobacteria. It was closely related to an unnamed Thiomonas strain (NO115) isolated from a Norwegian mining site, though sequence identities between strain b6T and characterized Thiomonas species were less than 95%. DNA–DNA hybridization between strain b6T and the type species of the genus Tm. intermedia showed less than 50% homology. On the basis of phylogenetic and phenotypic characteristics, strain b6T (DSM 16361T, LMG 22795T) is proposed as the type strain of the new species Thiomonas arsenivorans, sp. nov.
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Abbreviations
- MCSM:
-
Modified Cheni As-oxidizing population Selective Medium
References
Anderson G.L., Love M. and Zeider B.K. (2003). Metabolic energy from arsenite oxidation in Alcaligenes faecalis. J. Phys. IV France 107: 49–52
Battaglia-Brunet F., Dictor M.-C., Garrid F., Crouzet C., Morin D., Dekeyser K., Clarens M. and Baranger P. (2002). An As(III)-oxidizing bacterial population: selection, characterization, and performance in reactors. J. Appl. Microbiol. 93: 656–667
Battaglia-Brunet F., Duquesne K., Dictor M.-C., Garrido F., Bonnefoy V., Baranger P. and Morin D. (2003). Arsenite oxidizing Thiomonas strains isolated from different mining sites. Geophys. Res. Abst. 5: 11069
Bruneel O., Personné J.-C., Casiot C., Leblanc M., Elbaz-Poulichet F. Mahler B.J., Le Flèche A. and Grimont P.A.D. (2004). Mediation of arsenic oxidation by Thiomonas sp. in acid-mine drainage (Carnoules, France). J. Appl. Microbiol. 95: 492–499
Benson D., Bogusk M.S., Lipman D.J., Ostell J., Ouellette B.F., Rapp B.A. and Wheeler D.L. (1999). GenBank. Nucleic Acids Res. 27: 12–17
Cashion P., Holder-Franklin M.A., McCully J. and Franklin M. (1977). A rapid method for base ratio determination of bacterial DNA. Anal. Biochem. 81: 461–466
Coupland K., Battaglia-Brunet F., Hallberg K.B., Dictor M.-C., Garrido F. and Johnson D.B. (2004). Oxidation of iron, sulfur and arsenic in mine waters and mine wastes: an important role for novel Thiomonas spp. In: Tsezos M, Hatzikioseyian A & Remoudaki E. (eds). Biohydrometallurgy; a sustainable technology in evolution. National Technical University of Athens, Zografou, Greece, pp. 639–646
De Ley J., Cattoir H. and Reynaerts A. (1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12: 133–142
Dennison F., Sen A.M., Hallberg K.B. and Johnson D.B. (2001). Biological versus abiotic oxidation of iron in acid mine drainage waters: an important role for moderately acidophilic, iron-oxidizing bacteria. In: Ciminelli VST & Garcia O. Jr. (eds). Biohydrometallurgy: Fundamentals, Technology and Sustainable Development, part B. Elsevier, Amsterdam, pp. 493–501
Duquesne K. (2004). Rôle des bactéries dans la bioremédiation de l’arsenic dans les eaux acides de drainage de la mine de Carnoules. Thèse de doctorat Université de la Méditerranée, Aix-Marseille II
Escara J.F. and Hutton J.R. (1980). Thermal stability and renaturation of DNA in dimethylsulphoxide solutions: Acceleration of renaturation rate. Biopolymers 19: 1315–1327
Felsenstein J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evol. 39: 783–791
Hall T.A. (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic. Acids. Symp. Ser. 41: 95–98
Hallberg K.B. and Johnson D.B. (2003). Novel acidophiles isolated from moderately acidic mine drainage waters. Hydrometallurgy 71: 139–148
Huber H. and Stetter K.O. (1990). Thiobacillus cuprinus sp. nov., a novel facultatively organotrophic metal-mobilizing bacterium. Appl. Environ. Microbiol. 56: 315–322
Huss V.A.R., Festl H. and Schleifer K.H. (1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst. Appl. Microbiol. 4: 184–192
Jahnke K.-D. (1992). Basic computer program for evaluation of spectroscopic DNA renaturation data from GILFORD System 2600 spectrometer on a PC/XP/AT type personal computer. J. Microbiol. Methods. 15: 61–73
Jackson C.R., Langner H.W., Doahoe-Christiansen J., Inskeep W.P. and McDermott T.R. (2001). Molecular analysis of microbial community structure in an arsenite-oxidizing acidic thermal spring. Environ. Microbiol. 3: 532–542
Johnson D.B. and Hallberg K.B. (2005). Biogeochemistry of the compost bioreactor components of a composite acid mine drainage passive remediation system. Sci. Total Environ. 338: 81–93
Jukes T.H. and Cantor C.R. (1969). Evolution of protein molecules. In: Munro H.N. (eds). Mammalian Protein Metabolism. Academic Press, New York, pp. 211–232
Katayama-Fujimura Y. and Kuraishi H. (1983). Emendation of Thiobacillus perometabolis London and Rittenberg 1967. Int. J. Sys. Bacteriol. 33: 650–651
Lebrun L., Brugna M., Baymann F., Muller D., Lièvremont D., Lett M.-C. and Nitschke W. (2003). Arsenite oxidase, an ancient bioenergetic enzyme. Mol. Biol. Evol. 20: 686–693
London J. (1963). Thiobacillus intermedius nov. sp., a novel type of facultative autotroph. Arch. Microbiol. 46: 329–337
London J. and Rittenberg S.C. (1967). Thiobacillus perometabolis nov. sp., a non-autotrophic Thiobacillus. Arch. Microbiol. 59: 218–225
Lovley D.R. and Phillips E.J.P. (1987). Rapid assay for microbially reduced ferric iron in aquatic sediments. Appl. Environ. Microbiol. 53: 1536–1540
Maidak B.L., Cole J.R., Lilburn T.G., Parker C.T., Saxman P.R., Farris R.J., Garrity G.M., Olsen G.J., Schmidt T.M. and Tiedje J.M. (2001). The RDP-II (Ribosomal Database Project). Nucleic Acids Res. 29: 173–174
Mesbah M., Premachandran U. and Withman W. (1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J. Syst. Bact. 39: 159–167
Moreira D. and Amils R. (1997). Phylogeny of Thiobacillus cuprinus and other mixotrophic Thiobacilli: proposal for Thiomonas gen. nov. Int. J. Syst. Bacteriol. 47: 522–528
Saito N. and Nei M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 405–425
Salmassi T.M., Venkateswaren K., Satomi M., Nealson K.H., Newman D.K. and Hering J.G. (2002). Oxidation of arsenite by Agrobacterium albertimagni, AOL15, sp. nov., isolated from Hot Creek, California. Geomicrobiol. J. 19: 53–66
Santini J.M., Sly L.I., Schnagl R.D. and Macy J.M. (2000). A new chemolithoautotrophic arsenite-oxidizing bacterium isolated from a gold mine: phylogenetic, physiological, and preliminary biochemical studies. Appl. Environ. Microbiol. 66: 92–97
Santini J.M., Wen L.I.S.A., Comrie D., de Wulf-Durand P. and Macy J.M. (2002). New arsenite-oxidizing bacteria isolated from Australian gold mining environments – phylogenetic relationships. Geomicrobiol J. 19: 67–76
Shooner F., Bousquet J. and Tyagi R.D. (1996). Isolation, phenotypic characterization, and phylogenetic position of a novel, facultatively autotrophic, moderately thermophilic bacterium, Thiobacillus thermosulfatus sp. nov. Int. J. Syst. Bacteriol. 46: 409–415
Van de Peer Y. and De Wachter R. (1994). TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comp. Applic. Biosci. 10: 569–570
Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., Stackebrandt E., Starr M.P. and Trüper H.G. (1987). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int. J. Syst. Bacteriol. 37: 463–464
Weeger W.D., Lievremont D., Perret M., Lagarde F., Hubert J.C., Leroy M. and Lett M.C. (1999). Oxidation of arsenite to arsenate by a bacterium isolated from an aquatic environment. Biometals 12: 141–149
Acknowledgements
This is the BRGM contribution no. 03922. We are grateful to Dr Peter Schumann (DSMZ) for his kind participation with the chemotaxonomic analyses. We also thank Karin Dekeyser (GRAM S.A), Manuel Clarens and Arnaud Denamur for their clear-sighted help in selecting, isolating and identifying the strain.
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Battaglia-Brunet, F., Joulian, C., Garrido, F. et al. Oxidation of arsenite by Thiomonas strains and characterization of Thiomonas arsenivorans sp. nov.. Antonie Van Leeuwenhoek 89, 99–108 (2006). https://doi.org/10.1007/s10482-005-9013-2
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DOI: https://doi.org/10.1007/s10482-005-9013-2