Abstract
Pericytes are essential mural cells distinguished by their association with small caliber blood vessels and the presence of a basement membrane shared with endothelial cells. Pericyte interaction with the endothelium plays an important role in angiogenesis; however, very few tools are currently available that allow for the targeting of pericytes in mouse models, limiting our ability to understand their biology. We have generated a novel mouse line expressing tamoxifen-inducible Cre-recombinase under the control of the platelet-derived growth factor receptor β promoter: PDGFRβ-P2A-CreER T2. We evaluated the expression of the PDGFRβ-P2A-CreER T2 line by crossing it with fluorescent reporter lines and analyzed reporter signal in the angiogenic retina and brain at different time points after tamoxifen administration. Reporter lines showed labeling of NG2+, desmin+, PDGFRβ+ perivascular cells in the retina and the brain, indicating successful targeting of pericytes; however, signal from reporter lines was also observed in a small subset of glial cells both in the retina and the brain. We also evaluated recombination in tumors and found efficient recombination in perivascular cells associated with tumor vasculature. As a proof of principle, we used our newly generated driver to delete Notch signaling in perivascular cells and observed a loss of smooth muscle cells in retinal arteries, consistent with previously published studies evaluating Notch3 null mice. We conclude that the PDGFRβ-P2A-CreER T2 line is a powerful new tool to target pericytes and will aid the field in gaining a deeper understanding of the role of these cells in physiological and pathological settings.
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Introduction
Pericytes comprise a fundamental part of the mural cell populations that is associated with small caliber vessels. They play key roles during angiogenesis in the development and stability of the vasculature, as well as in the formation and maintenance of the blood brain barrier (BBB) [1, 2]. Additionally, abnormalities in pericyte function have been linked to pathologies such as diabetic retinopathy [3], fibrosis [4], Alzheimer’s disease [5], and stroke [6]. Pericytes are also commonly detected in the tumor microenvironment, where they have been involved in promoting tumor vessel stability [7] and limiting tumor metastasis [8, 9].
To date, limited tools are available to study pericytes partly due to the lack of a unique marker for their identification [1]. Current strategies to identify pericytes rely on their morphology, their proximity to endothelium, and in the best case, assessment of several well-established makers such as NG2, desmin, CD13, or platelet-derived growth factor receptor β (PDGFRβ). Efforts to study pericyte biology using NG2-CreER mice [10] have resulted in efficient linage tracing of pericytes in the developing heart [11]. However, the use of this mouse line is limited in brain tissue, where NG2 is also expressed in oligodendrocyte progenitor cells [10]. PDGFRβ has been suggested as a useful marker for brain pericytes [1]. The use of constitutively active PDGFRβ-Cre mice [12] [13] results in efficient targeting of the mural cell populations (pericytes and smooth muscle cells). However, these drivers also target additional progenitor populations that give rise to non-mural linages [12] and, more importantly, blood and lymphatic endothelium, raising concern over their use in vascular studies [14].
In order to study the functions of pericytes during postnatal angiogenesis, and to bypass the confounding effects that constitutive PDGFRβ-Cre drivers may have on unrelated progenitor cells during development, we generated a tamoxifen-inducible PDGFRβ-P2A-CreER T2 mouse driver. Here, we present the characterization of this driver in different angiogenic postnatal vascular beds.
Materials and methods
Mice
All mice received humane care following the guidelines of the National Institute of Health’s Guide for the Care and Use of Laboratory Animals. Additionally, all animal experiments were approved by the Institution of Animal Care and Use Committee (IACUC) at Columbia University (New York) or the Animal Care Committee (ACC) at the University of Illinois at Chicago.
Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo (Rosa-mT/mG) [15] and Gt(ROSA)26Sortm9(CAG-tdTomato)Hze (Rosa-tdTomato) [16] were obtained from Jackson Laboratories. PDGFRβ-P2A-Cre T2 mice (recently deposited at The Jackson Laboratory as Stock No. 030201) were generated by replacing the translation stop codon (TAG) of the Pdgfrb gene on RP23-106H12 BAC clone with a 4445-bp P2A-CreERT2-FNF cassette by BAC recombineering. A DNA fragment containing 2 kb upstream of P2A-CreERT2-FNF cassette insertion and a 4.8-kb sequence downstream of the P2A-CreERT2-FNF cassette were inserted into plasmid pMCS-DTA (a generous gift from Dr. Kosuke Yusa of Osaka University, Japan) to generate the Pdgfrb-P2A-CreERT2-FNF-gene-targeting vector, pMCS-Pdgfrb-P2A-CreERT2-FNF-DTA. This targeting vector was linearized and electroporated into KV1 (129-C57BL/6 hybrid) ES cells, and targeted ES clones were injected into C57BL/6N blastocysts to generate male chimeras. Male chimeras were bred to ACTB-Flpe females (Jackson Lab Stock No: 005703) to transmit the Pdgfrb-P2A-CreERT2 allele and to remove the neo cassette at the same time (Fig. 1). Primers used to verify the successful removal of the neo cassete: P1: ctctctctctgcctccctcagctat, P2: acggacagaagcattttccaggtat, P3: ttgatatcgaattcccgaagttcct, P4: cctccccacctctcctctagtttta.
Cre-recombinase activity was induced by delivery of a solution of Tamoxifen (Sigma) in Corn Oil (Sigma). For analysis of brain and retina at postnatal (P) day 5, tamoxifen was delivered through maternal milk by administering 250 μg/kg to the nursing mom at P1, P2, and P3.
For experiments involving a time-course activation of Cre-recombinase, 250 μg/kg was administered to the nursing mom in a single dose at P0, and analysis of the retina was performed at P2, P5 and P14.
For experiments involving tumor implantation, and adult time-course activation of Cre, tamoxifen was injected at 6 weeks intraperitoneally 2 mg/mouse/day during 5 consecutive days.
Tumor cell growth and implantation
Lewis lung carcinoma (LLC) cell line was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 100 u/ml of penicillin–streptomycin. 5 × 105 LLC cells were injected subcutaneously in the lower flank of 8-week mice (2 weeks after tamoxifen injection).
Immunofluorescence
After euthanasia, organs were harvested and fixed in a 4% formaldehyde solution in phosphate-buffered saline (PBS).
For retinal staining, eyes were fixed for 1–2 h at 4 °C and then transferred to PBS. Retinas were then dissected and permeabilized in 1% bovine serum albumin (BSA), 0.5% Triton X-100 in PBS overnight (O/N) at 4 °C, and washed with PBLEC buffer (1% Triton X-100, 0.1 mmol/L MgCl2, 0.1 mmol/L CaCl2, 0.1 mmol/L MnCl2 in PBS pH6.8). The following primary antibodies/lectin were used O/N at 4 °C in PBLEC: biotin-conjugated isolectin B4 (Vector Laboratories) 1:50, anti-NG2 (Millipore) 1:500, anti-desmin (AbCam) 1:500, anti-PDGFRβ (Cell Signaling or R&D Systems) 1:100, anti-smooth muscle actin (Sma) 1:500 (Sigma), and FITC-conjugated anti-Sma (Sigma) 1:200. Of note, anti-Sma antibodies target the protein encoded by the Acta2 gene. After washes, detection of primary antibodies/lectin was performed with streptavidin-conjugated Alexa Fluor 488 or Alexa 647 (Invitrogen) 1:500 and Alexa Fluor 488-, 594-, or 647-conjugated secondary antibodies (Invitrogen) 1:700 for 2 h at room temperature (R.T.). Samples were post-fixed with 4% formaldehyde and flat-mounted.
For brain and tumor, samples were fixed at 4 °C O/N and then immersed in 30% sucrose solution for 24 h. Samples were then embedded in Tissue-Tek OCT compound (Takura). Immunostaining was performed O/N at 4 °C in cryosections using the following primary antibodies/lectin: biotin-conjugated Isolectin B4 (Vector Laboratories) 1:50, anti-NG2 (Millipore) 1:500, anti-desmin (AbCam) 1:500, anti-PDGFRβ (Cell Signaling or R&D Systems) 1:100, anti-Endomucin (Santa Cruz) 1:200, and the following secondary reagents: streptavidin-conjugated Alexa Fluor 647 (Invitrogen) 1:500 and Alexa Fluor 488-, 594-, or 647-conjugated secondary antibodies (Invitrogen) 1:700 for 1 h at R.T.
All samples were mounted using Vectashield with DAPI (Vector Laboratories). Images were acquired using Zeiss LSM 700, 880 or Nikon A1RMP confocal microscope and analyzed and processed using FiJi.
Results and discussion
In order to generate the PDGFRβ-P2A-CreER T2 mouse line, we inserted a sequence derived from the porcine teschovirus-1 P2A gene that allows “ribosome skipping” [17] between the Pdgfrb and CreER T2 coding sequences (Online Resource 1). Thus, the PDGFRβ-P2A-CreER T2 sequence in the construct allows for production of both PDGFRβ and CreERT2 at similar expression levels via ribosome skipping. This approach has been found to be more efficient at producing comparable levels of two functional proteins from one mRNA than use of an internal ribosomal entry (IRES) site [17].
To analyze the efficiency of tamoxifen-mediated induction and the expression pattern of Cre-recombinase in PDGFRβ-P2A-CreER T2 mice, we crossed it with two different Cre-recombinase mouse reporter lines: Rosa-mT/mG [15] and Rosa-tdTomato [16]. The Rosa-mT/mG reporter mice are engineered to express membrane-targeted tdTomato fluorescent protein in the absence of Cre-recombinase; however, when Cre-recombinase is present, the tdTomato expression cassette is excised allowing for the expression of membrane-targeted eGFP (enhanced green fluorescent protein). Rosa-tdTomato reporter mice drive tdTomato expression upon Cre-mediated excision of a floxed STOP cassette [16].
To determine whether PDGFRβ-P2A-CreER T2 targets recombination to pericytes, we analyzed the postnatal retina, a well-characterized active angiogenesis system that permits the study of mural cells in different vascular compartments: newly formed capillary plexus, mature arteries, and veins. We crossed PDGFRβ-P2A-CreER T2 to Rosa-tdTomato mice and administered tamoxifen by oral gavage to the nursing mom at postnatal (P) day 1, 2, and 3. At P5, we evaluated the expression of tdTomato protein in the retina by whole-mount analysis. Retinas were immunostained with isolectin-B4 (IB4) to identify blood vessels, and with NG2, a marker expressed in pericytes. Expression of the tdTomato reporter was observed associated with the NG2-expressing cells lining the vascular network (Fig. 1a–d) with an efficiency of recombination in these cells of 84.17 ± 3.48% (n = 3). We sought to determine whether the tdTomato reporter signal was observed in cells expressing PDGFRβ, since Cre expression should be driven by the Pdgfrb promoter. Immunostaining against PDGFRβ revealed that the distribution pattern of this receptor was associated with NG2+ pericytes in a similar fashion to that of the tdTomato reporter protein (Fig. 1e–h), indicating a genuine expression of Cre-recombinase in PDGFRβ+ cells. As expected considering the previously described pattern of expression of PDGFRβ [18], fluorescent signal from the tdTomato reporter was present in the different vascular segments of the retina: arterioles (identified by Sma), capillaries, and venules (Fig. 1i–l). Of note, we also observed additional weak expression of the reporter in a small subset of retinal glial cells (data not shown). Taken together, these results indicate faithful expression of the PDGFRβ-P2A-CreER T2 construct and efficient recombination upon tamoxifen induction in PDGFRβ+ cells in the postnatal retina.
In parallel, we also crossed PDGFRβ-P2A-CreER T2 with a different reporter line, Rosa-mT/mG. As described above, we administered tamoxifen by oral gavage to the nursing mom at P1, P2, and P3, and analyzed the retinal vasculature at P5. Similar to the results observed with the previous reporter, staining with anti-PDGFRβ showed that eGFP expression from the Rosa-mT/mG reporter was associated with PDGFRβ+ cells (Online Resource 2a-d), further validating expression of Cre-Recombinase in PDGFRβ+ cells. Additionally, as observed with the tdTomato reporter, eGFP signal from the Rosa-mT/mG reporter was detected in the perivascular cells in arterioles, capillaries, and venules (Online Resource 2e-h). When retinas were stained with anti-NG2 to label pericytes, we observed a clear co-localization of eGFP with NG2+ cells indicating a successful targeting of pericytes (Online Resource 2i-p). However, we observed that efficiency of recombination in pericytes using this reporter was less than that observed using the tdTomato reporter, in this case 41.94 ± 18.67% (n = 5) of NG2+ cells were GFP+. Discrepancies between recombination efficiency in different mouse reporter lines (including Rosa-tdTomato and Rosa-mT/mG) have already been described by Liu et al. [19], and the differences we observed in efficiency are similar to what has been previously noted. Liu et al. proposed that their results imply that reporter sensitivity inversely correlates with the distance between the LoxP sites, a theory that is consistent with previous observations by Coppoolse et al. [20].
To further our understanding of the PDGFRβ-P2A-CreER T2 mouse line, we performed a time-course activation of the Cre-Recombinase. We treated PDGFRβ-P2A-CreER T2; Rosa-tdTomato moms with a single dose of tamoxifen on the day of birth (P0) and analyzed the retinal vasculature of the pups at different times 48 h (P2), 5 days (P5), and 2 weeks (P14) after tamoxifen delivery. Expression analysis of the tdTomato reporter in NG2+ cells in P2 mice revealed that recombination was already highly efficient at this early time point (Fig. 2a–d). Similarly, analysis at 5 days and 2 weeks after tamoxifen administration showed widespread labeling of perivascular cells (NG2+) in the arterioles, capillaries, and venules (Fig. 2e–l). The observed efficient recombination of the PDGFRβ-P2A-CreER T2 mouse line at the analyzed time points makes it a very attractive tool to study the role of perivascular cells at the different stages of angiogenesis: P2, when the primary vascular plexus is starting to form; P5, when the vascular plexus is actively remodeling into arterioles and venules; and P14, when the deeper plexuses of the retina are developing [21].
We also evaluated the expression of the PDGFRβ-P2A-CreER T2 mouse line in the retina of adult mice. Six-week-old PDGFRβ-P2A-CreER T2; Rosa-tdTomato mice were treated with tamoxifen intraperitoneally for five consecutive days, and the retinal vasculature was evaluated at 8 weeks (2 weeks later). TdTomato signal from the reporter was detected in the perivascular cells of the retina (NG2+), but also in additional cells present in the retina (Fig. 2m–p). Similar to P5 retinas, 8-week-old mice also showed expression of the tdTomato reporter in smooth muscle cells in the retina and in bigger caliber vessels (data not shown). Detection of the reporter in smooth muscle cells was expected, as smooth muscle cells have been described to express PDGFRβ; however, this lack of pericyte specificity is a potential limitation of the model since the PDGFRβ promoter is active in both pericytes and smooth muscle cells. These results indicate that, while the PDGFRβ-P2A-CreER T2 mouse line is very efficient at targeting perivascular cells also in the adult retina, the expression of this Cre-driver in other populations should be taken into account when using this line in experimental settings.
Considering the importance that pericytes have in the brain vasculature, we characterized the pattern of expression of PDGFRβ-P2A-CreER T2 in the murine brain. Brain sections from tamoxifen-induced PDGFRβ-P2A-CreER T2; Rosa-tdTomato mice at P5 showed high expression of the tdTomato reporter in NG2+, PDGFRβ+ cells lining the blood vessels in the cortex at P5 (Fig. 3a–d), indicative of Cre expression in pericytes. Since NG2 at this time point is also expressed by other brain populations, we further confirmed the pericytic nature of the perivascular tdTomato+ cells by co-staining with anti-desmin, which also showed co-localization with the reporter (Fig. 3e–h). Of note, in addition to the tdTomato reporter expression in perivascular cells, we observed scattered signal, though weaker, in other populations in the brain (data not shown).
We also analyzed P5 brain sections from tamoxifen-induced PDGFRβCreER T2 mice crossed with the Rosa-mT/mG reporter. We observed perivascular expression of the eGFP protein and co-localization with anti-desmin staining (Online resource 3) similar to that observed with the tdTomato reporter. Consistent with the differential reporter efficiency observed in the retina, lower efficiency of recombination was also observed in the brain with the Rosa-mT/mG reporter when compared to Rosa-tdTomato. Taken together, our overall analysis of the reporter expression in the brain from reporter mice crossed with PDGFRβ-P2A-CreERT2 indicates successful targeting of the brain pericytes.
Tumor angiogenesis remains one of the most clinically relevant angiogenic processes, and pericytes are a critical component of the tumor microenvironment. Similar to developmental angiogenesis, pericytes are recruited by tumor vessels during tumor growth and angiogenesis [22]. We therefore interrogated whether we could target the pericyte populations in tumors using PDGFRβ-P2A-CreER T2 mice. For this purpose, 6-week PDGFRβ-P2A-CreER T2; Rosa-tdTomato adult mice were treated with tamoxifen, and 2 weeks later Lewis lung carcinoma (LLC) tumors were grown subcutaneously in the lower left flank. After dissection, we evaluated tumor sections by immunostaining with anti-Endomucin to label the tumor endothelium. Our results revealed tdTomato reporter signal in perivascular populations lining Endomucin+ tumor vessels (Fig. 4), indicating an efficient recombination of pericytes in this tumor model. However, while our data using LLC tumors points to successful targeting of tumor pericytes utilizing our novel PDGFRβ-P2A-CreER T2 mouse line, there is a considerable degree of heterogeneity in the pericyte coverage and marker expression in different tumor types [1, 23], and hence further studies might be needed to determine the specificity of the PDGFRβ-P2A-CreER T2 mouse line in alternative tumor models.
As a proof of principle that the PDGFRβ-P2A-CreER T2 mouse line is an efficient tool to drive genetic modifications in perivascular cells, we used it to specifically remove Notch signaling in this population. Notch signaling plays an important role in mural cells, which primarily express the Notch3 receptor [1]. Studies evaluating Notch3 null mice revealed decreased smooth muscle cell coverage of the arteries likely due to an abnormal maturation of these cells [24, 25]. For our studies, we crossed PDGFRβ-P2A-CreER T2 mice with Rbpj flox/flox mice. Rbpj is a transcription factor acting downstream of Notch and is critical to mediate its signaling [26]. Tamoxifen was given to the nursing moms at P1, P2, P3, and we evaluated the retina of PDGFRβ-P2A-CreER T2; Rbpj flox/flox mice and control littermates (PDGFRβ-P2A-CreER T2 ; Rbpj flox/wt) at 6 weeks of age. As expected, we detected a marked lack of Sma+ cells in the arterioles of PDGFRβ-P2A-CreER T2; Rbpj flox/flox when compared to PDGFRβ-P2A-CreER T2; Rbpj flox/wt littermates (Online Resource 4). These results show how inducing the loss of Notch signaling in perivascular cells using the PDGFRβ-P2A-CreER T2 mouse line results in a similar phenotype (reduced presence of Sma+ cells) to that observed in the global Notch3 null mice, and further validate the use of the PDGFRβ-P2A-CreER T2 to efficiently target specific genes in perivascular cells.
In summary, we have generated a novel transgenic mouse line expressing Cre-recombinase in a tamoxifen-dependent fashion under the control of the PDGFRβ promoter. Our results comparing two different reporter lines underscore the importance of validating the excision of the floxed gene of interest, since efficiency may vary with different targets. Nevertheless, we have shown how the PDGFRβ-P2A-CreER T2 line provides a powerful tool to target pericytes in developmental and pathological settings. Additionally, successful tamoxifen-mediated Cre induction was observed in early postnatal and also in adult mice, highlighting the versatility of this mouse line for its use in multiple vascular angiogenic environments.
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Acknowledgements
We would like to thank Valeriya Borisenko for her assistance in mouse husbandry, and Hui-Chuan Hung for her assistance in the microinjection to generate the germline chimera and mouse breeding. This work was funded by NIH Grant R01HL112626 (J.K.), and NCI P30CA013696-40 (C.S.L).
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C-SL, ML, and JK conceptualized and designed the PDGFRβ-P2A-CreER T2 mice. ML generated the PDGFRβ-P2A-CreERT2 targeting vector. FL performed the gene targeting and identified the targeted ES cells for the generation of the PDGFRβ-P2A-CreER T2 mice. HC, BP, and TN collected and interpreted the data on the characterization of the PDGFRβ-P2A-CreER T2 with the two reporter mouse lines. HC drafted the manuscript.
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This article does not contain any studies with human participants performed by any of the authors. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted as described above.
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Cuervo, H., Pereira, B., Nadeem, T. et al. PDGFRβ-P2A-CreERT2 mice: a genetic tool to target pericytes in angiogenesis. Angiogenesis 20, 655–662 (2017). https://doi.org/10.1007/s10456-017-9570-9
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DOI: https://doi.org/10.1007/s10456-017-9570-9