Abstract
Objective
To approach the expressions of MDR1 and BCRP in breast cancer stem cells and differentiated cells.
Methods
The breast cancer stem cells were separated from human breast cancer primary tissues and MCF-7 by flow cytometry. Then we measured the expressions of MDR1 and BCRP with different subset cells by Realtime-PCR.
Results
Contrasted with breast cancer differentiated cells, the expressions of MDR1 and BCRP in breast cancer stem cells were higher (P < 0.01), and the proportion of stem cells rose after chemotherapy (P < 0.01).
Conclusion
Contrasted with breast cancer differentiated cells, breast cancer stem cells have stronger ability of drug-resistance with higher level of multi-drug resistance genes, and it is one of key points for chemotherapy failure of breast cancer.
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Supported by grants from National Natural Science Foundation Project (No. 30571798) and the Major Scientific and Technological Research Project of 11th Five-Year Plan of Hubei Province (No. 2006AA301A05).
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Li, Z., Liu, C., He, Y. et al. The expression and significance of multi-drug resistance genes in breast cancer stem cells. Chin. -Ger. J. Clin. Oncol. 7, 538–541 (2008). https://doi.org/10.1007/s10330-008-0074-3
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DOI: https://doi.org/10.1007/s10330-008-0074-3