Abstract
Objective: To detect the style of K-ras gene point mutation in human pancreatic cancer cell line PANC-1 and decide the bp sequence of Ras target position interfered by RNA. Methods: Three kinds of special sequence primers (SSP) for polymerase chain reaction (PCR) with regard to the mutation styles (GAT, CGT and GGT) at codon 12 of K-ras were used to study the human pancreatic cancer cell line PANC-1. The amplification products were studied with polyacrylamine gel electrophoresis to detect the style of point mutation. Results: The style of K-ras gene point mutation at codon 12 was GAT in human pancreatic cancer cell line. Conclusion: PCR-SSP is rapid, convenient and high specific. The results provide a basis for further gene therapy by RNA interference for pancreatic cancer.
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WANG, W., WANG, C., DONG, J. et al. Detection of K-ras Gene Point Mutation's Style in Human Pancreatic Cancer Cell Line PANC-1 by PCR-SSP. Chinese-German J Clin Oncol 5, 46–48 (2006). https://doi.org/10.1007/s10330-005-0355-z
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DOI: https://doi.org/10.1007/s10330-005-0355-z