Abstract
Ralstonia solanacearum OE1-1 (OE1-1) induced necrotic lesions in an infiltrated area of tobacco leaves 72 h after infiltration, and the leaves had wilted at 5 days. Here we report phenotypes of the OE1-1 mutant deleted hrpB or hrpY with respect to colonization and proliferation in infiltrated tobacco leaves and the induction of host responses immediately after invasion. An hrpB-deleted mutant and an hrpY-deleted mutant grew similar to the parent strain, OE1-1, in vitro. When infiltrated into tobacco leaves, the mutants lost their ability to induce necrotic lesions and provoke the disease. Populations of the mutants in the infiltrated area were retained equally after infiltration, and the mutants were not detected in any other region. Transcripts of hsr203J and hin1, which are marker genes of plant–microbe interactions and were detected 8 h after infiltration of OE1-1 in the infiltrated area of tobacco leaves, were not detected in the mutant-infiltrated tobacco leaves. These results suggest that the hrp mutants, which are deficient in type III secretion machinery, lose their ability to colonize and multiply in host plants immediately after invasion, resulting in a loss of their ability to induce host responses and the subsequent provocation of disease.
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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB094669
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Kanda, A., Ohnishi, S., Tomiyama, H. et al. Type III secretion machinery-deficient mutants of Ralstonia solanacearum lose their ability to colonize resulting in loss of pathogenicity. J Gen Plant Pathol 69, 250–257 (2003). https://doi.org/10.1007/s10327-003-0041-3
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DOI: https://doi.org/10.1007/s10327-003-0041-3