Abstract
An efficient system was developed for direct plant regeneration from in vitro-derived leaf explants of Embelia ribes Burm. f., a vulnerable medicinal woody climber of the Western Ghats of India. The in vitro procedure involved three steps that included induction of shoot initials from leaf tissue, regeneration and elongation of shoots from the shoot initials, and rooting of shoots. The induction of shoot initials was achieved on Murashige and Skoog (MS) solid medium supplemented with different concentrations of thidiazuron (TDZ). The best medium for shoot induction was MS with 0.272 µM TDZ. Numerous shoot primordia developed within 2–3 weeks on the leaf margin as well as on the midrib region, without any callus phase. In the second step, the shoot clumps separated from the leaf explant on transfer to MS basal medium, resulting in the differentiation of 90% of the shoot initials into well-developed shoots. The 2- to 3-cm-long shoots rooted on half-strength MS basal medium supplemented with 4.90 µM indole-3-butyric acid (IBA) and 3% (w/v) sucrose in the third stage. The rooted plants could be established in soil with 70% success. This protocol could be utilized for in vitro propagation and conservation of this important threatened medicinal plant.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
InstitutionalAuthorNameAnon (1966) The pharmacopoeia of India Government of India Press Nasik 795
Anon. (1990) The ayurvedic pharmacopoeia of India, part I vol II. Ministry of Health and Family Welfare, Government of India, pp 123–124
InstitutionalAuthorNameAnon (2002) The wealth of India – raw materials. National Institute of Science Communication, CSIR New Delhi India 74–75
NV Dalal RV Rai (2004) ArticleTitleIn vitro propagation of Oroxylum indicum Vent.: a medicinally important forest tree J For Res 9 61–65 Occurrence Handle10.1007/s10310-003-0055-x Occurrence Handle1:CAS:528:DC%2BD2cXjsFegs74%3D
G Das GR Rout (2002) ArticleTitleDirect plant regeneration from leaf explants of Plumbago species Plant Cell Tiss Org 68 311–314 Occurrence Handle1:CAS:528:DC%2BD38XitFehtL8%3D
XF Gu JR Zhang (2005) ArticleTitleAn efficient adventitious shoot regeneration system for Zhanhua winter jujube (Zizyphus jujuba Mill.) using leaf explants Plant Cell Rep 23 775–779 Occurrence Handle10.1007/s00299-005-0920-5 Occurrence Handle1:CAS:528:DC%2BD2MXitFCgt7o%3D Occurrence Handle15690160
PD Hare J Van Staden (1994) ArticleTitleInhibitory effect of Thidiazuron on the activity of cytokinin oxidase isolated from soybean callus Plant Cell Physiol 35 1121–1125 Occurrence Handle1:CAS:528:DyaK2MXislaitr4%3D
CA Huetteman JE Preece (1993) ArticleTitleThidiazuron: a potent cytokinin for woody plant tissue culture Plant Cell Tiss Org 33 105–119 Occurrence Handle1:CAS:528:DyaK3sXltFegsrk%3D
HS Lin MJ De Jeu E Jacobsen (1997) ArticleTitleDirect shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L Plant Cell Rep 16 770–774 Occurrence Handle10.1007/s002990050317 Occurrence Handle1:CAS:528:DyaK2sXmtFWmur4%3D
C Magioli APM Rocha DE de Oliveira E Mansur (1998) ArticleTitleEfficient shoot organogenesis of eggplant (Solanum melongena L.) induced by Thidiazuron Plant Cell Rep 17 661–663 Occurrence Handle1:CAS:528:DyaK1cXivFylu7o%3D
Mitra R (1995) ‘Vidanga’ (Embelia ribes) – an Ayurvedic drug can help family planning. Applied Botany Abstracts, National Botanical Research Institute, Lucknow, India
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol Plant 15 473–497 Occurrence Handle1:CAS:528:DyaF3sXksFKm
JE Preece MR Imel (1991) ArticleTitlePlant regeneration from leaf explants of Rhododendron PJM hybrids Sci Hortic 48 159–170 Occurrence Handle10.1016/0304-4238(91)90163-S Occurrence Handle1:CAS:528:DyaK38XhsVygt7c%3D
Ravikumar K, Ved DK (2000) Hundred red listed medicinal plants of conservation concern in Southern India, 1st edn. Foundation for Revitalization of Local Health Traditions (FRLHT), Anugraha, Bangalore, India
VV Sivarajan I Balachandran (1994) Ayurvedic drugs and their plant sources Oxford and IBH New Delhi, India 267–269
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Raghu, A., Geetha, S., Martin, G. et al. Direct shoot organogenesis from leaf explants of Embelia ribes Burm. f.: a vulnerable medicinal plant. J For Res 11, 57–60 (2006). https://doi.org/10.1007/s10310-005-0188-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10310-005-0188-1