Abstract
To evaluate the potential of the production of the ectomycorrhizal fungus Tricholoma matsutake to produce carbohydrases, (1) the distribution of carbohydrase activities among the different strains (18 strains) was investigated and (2) the abilities of T. matsutake and saprophytic fungi to produce β-glucosidase were compared. The results showed that the carbohydrase productions patterns of T. matsutake still resemble one another. Moreover, this fungus exhibited markedly higher β-glucosidase than did the saprophytic mushrooms. Tricholoma matsutake showed weak production of α-amylase and α-glucosidase in a static cultur filtrate. On the other hand, glucoamylase activity was not observed. Surprisingly, we discovered that β-glucosidase demonstrated strong activity. This finding suggests that this fungus has saprotrophic abilities. The carbohydrase production systems in T. matsutake were characterized from our experimental results. Also, we point out some weak points in the carbohydrase production systems of T. matsutake.
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References
Futai K (2003) Matsu-gare ha mori no kansen-sho (in Japanese). Bunichi, Tokyo, pp 13–54
Hur T-C, Ka K-H, Joo S-H, Terashita T (2001) Characteristics of the amylase and its related enzymes produced by ectomycorrhizal fungus Tricholoma matsutake. Mycobiology 29:183–189
Hirato H, Kitamoto Y (1995) Effect of physico-chemical characteristics of the culture substrate and nutritional conditions on mycelial growth in Tricholoma matsutake (in Japanese). Mushroom Sci Biotechnol 2:67–72
Inaba K, Yoshida T, Takano Y, Mayuzumi Y, Mitsunaga T, Koshijima T (1995) An instance of the fruiting-body formation of Tricholoma matsutake. Environmental Control in Biology 33:59–64
Ishihara H, Imamura K, Kita M, Aimi T, Kitamoto Y (2005) Enhancement of the viscometric endocellulase activity of Polyporus arcularius CMCase IIIa by cellobiose and cellooligosaccharides. Mycoscience 46:148–153
Kawai M, Abe S (1976) Studies on the artificial reproduction of Tricholoma matsutake (S. Ito et Imai) Sing. I. Effects of carbon and nitrogen sources in media on the vegetative growth of T. matsutake (in Japanese). Trans Mycol Soc Jpn 17:159–167
Kusuda M, Nagai M, Hur T, Ueda M, Terashita T (2003) Purification and some properties of α-amylase from a ectomycorrhizal fungus, Tricholoma matsutake. Mycoscience 44:311–317
Kusuda M, Ueda M, Konishi Y, Matsuzawa K, Shirasaka N, Nakazawa M, Miyatake K, Terashita T (2004) Characterization of extracellular glucoamylase from the ectomycorrhizal mushroom Lyophyllum shimeji. Mycoscience 45:383–389
Kusuda M, Ueda M, Konishi Y, Araki Y, Yamanaka K, Nakazawa M, Miyatake K, Terashita T (2006) Detection of β-glucosidase as a saprotrophic ability from a ectomycorrhizal mushroom, Tricholoma matsutake. Mycoscience 47:184–189
Kusuda M, Ueda M, Konishi Y, Yamanaka K, Terashita T, Miyatake K (2007) Effects of carbohydrate substrate on the vegetative mycelial growth of an ectomycorrhizal mushroom, Tricholoma matsutake, isolated from Quercus. Mycoscience 48:358–364
Lee C-Y, Hong O-P, Jung M-J, Han Y-H (1998) The extracellular enzyme activities in culture broth of T. matsutake (in Korean). Korean J Mycol 26:496–501
Ohnishi Y, Nagase M, Ichiyanagi T, Kitamoto Y, Aimi T (2007) Transcriptional regulation of two cellobiohydrolase encoding genes (ce11 and ce12) from the wood-degrading basidiomycete Polyporus arcularius. Appl Microbiol Biotechnol 76:1069–1078
Ohta A (1994) Purification of fruit-bodies of a mycorrhizal fungus, Lyophyllum shimeji, in pure culture. Mycoscience 35:147–151
Ohta A (1997) Ability of ectomycorrhizal fungi to utilize starch and related substrates. Mycoscience 38:403–408
Somogyi M (1952) Note on sugar determination. J Biol Chem 195:19–23
Terashita T, Kono M, Yoshikawa K, Shishiyama J (1995) Productivity of hydrolytic enzymes by mycorrhizal mushrooms. Mycoscience 36:221–225
Terashita T, Kusuda M, Matsukawa S, Nagai M, Yoshikawa K, Sakai T (2000)Production of extracellular amylase from Tricholoma matsutake and its properties on starch hydrolysis (in Japanese). Mushroom Sci Biotechnol 8:115–120
Varrio L-M, Guerin LA, Matsushita N, Suzuki K, Lapeyrie F (2002) Saprobic potential of Tricholoma matsutake: growth over pine bark treated with surfactants. Mycorrhiza 12:1–5
Watanabe K, Kawai M, Obatake Y (1994) Fruiting body formation of Lyophyllum shimeji in pure culture. Mokuzai Gakkaishi 40:879–882
Yamada A (2005) To the artificial cultivation: the present situation and the approach on the developmental research (in Japanese). Tech Innov 15:24–28
Yamanaka K, Tanaka T, Nakanishi J, Cao H, Chen M (2003) Tricholoma matsutake in East Tibet, Sichuan, China (in Japanese). In: 47th Annual Meeting of the Mycological Society of Japan, Sapporo, May 31–June 1, p 51
Yoshida H, Fujimoto S (1994) A trial cultivation of Lyophyllum shimeji on solid media (in Japanese). Trans Mycol Soc Jpn 35:192–195
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Kusuda, M., Ueda, M., Miyatake, K. et al. Characterization of the carbohydrase productions of an ectomycorrhizal fungus, Tricholoma matsutake . Mycoscience 49, 291–297 (2008). https://doi.org/10.1007/s10267-008-0423-7
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DOI: https://doi.org/10.1007/s10267-008-0423-7