Abstract
The mist culture system was conducted to study secreted polysaccharides from soybean (Glycine max) roots grown for 15 days. Roots were rinsed with distilled water (DW) for 15 min, then with 30 mM oxalic acid (OXA) for 15 min to remove ionically bound sugar. Released sugars were further fractionated into low (L) and high (H) molecular weight fractions with Sephadex G-10. DW rinsing released 190 μg neutral sugar (NS) and 62 μg uronic acid (UA) per plant, while 374 μg NS and 70 μg UA per plant were released by OXA rinsing. Acetylation analysis revealed that the L fraction by DW and OXA mainly consisted of glucose (Glc), pinitol, and UA, whereas the H fraction mainly consisted of arabinose (Ara), galactose (Gal), Glc, and UA. The presence of rhamnose (2%–6%) in both fractions suggests secretion of rhamnogalacturonans. Methylation analysis revealed that the H fraction by DW and OXA contained T-Ara, 3-, 6-, and 3,6-Gal, suggesting the presence of type II arabinogalactan and arabinogalactan proteins. HPLC analysis detected mono-, di-, and tri-GalA in the L fraction by DW and OXA. Substances corresponding to sucrose, kojibiose, cello- and laminari-oligosaccharides were also found in root exudates.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: August 14, 2001 / Accepted: October 29, 2001
Rights and permissions
About this article
Cite this article
Timotiwu, P., Sakurai, N. Identification of mono-, oligo-, and polysaccharides secreted from soybean roots. J Plant Res 115, 0077–0085 (2002). https://doi.org/10.1007/s102650200012
Issue Date:
DOI: https://doi.org/10.1007/s102650200012