Abstract
The phylogenetic relationships among the fishes in the perciform tribe Epinephelini (Serranidae) have long been poorly understood, in large part because of the numerous taxa that must be considered and the large, circumtropical distribution of the group. In this study, genetic data from two nuclear (Tmo-4C4 and histone H3) and two mitochondrial (16S and 12S) genes were gathered from 155 serranid and acanthomorph species as a means of developing a phylogenetic hypothesis using both maximum-likelihood and -parsimony criteria. The maximum-parsimony analysis recovered 675 most parsimonious trees of length 5703 steps (CI = 0.2523, HI = 0.7477, RI = 0.6582), and the maximum-likelihood analysis recovered 1 tree at −lnLikelihood = 28279.58341. These phylogenetic hypotheses are discussed in light of previous morphological evidence to evaluate the evolutionary history of the group and their implications for the currently recognized taxonomy. Our results question the monophyly of the Serranidae, as well as the genera Cephalopholis, Epinephelus, and Mycteroperca as currently defined. The Serranidae is monophyletic only with the exclusion of the genera Acanthistius and Niphon. We propose a revised classification of the tribe Epinephelini that reflects the hypothesized shared ancestry of the group and recognizes 11 genera: Alphestes, Cephalopholis, Dermatolepis, Epinephelus, Gonioplectrus, Hyporthodus (which is resurrected for 11 species of deep-bodied groupers), Mycteroperca (including 7 species heretofore allocated to Epinephelus), Plectropomus, Saloptia, Triso, and Variola.
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References
CC Baldwin GD Johnson (1993) ArticleTitlePhylogeny of the Epinephelinae (Teleostei: Serranidae) Bull Mar Sci 52 240–283
LS Berg (1940) ArticleTitleClassification of fishes, both recent and fossil Trav Inst Zool Acad Sci USSR 5 87–517
MA Bostrom BB Collette BE Luckhurst KS Reece JE Graves (2002) ArticleTitleHybridization between two serranids, the coney (Cephalopholis fulva) and the creole-fish (Paranthias furcifer), at Bermuda Fish Bull 100 651–661
MF Cervigón E Velasquez (1966) ArticleTitleLas especies del genero Mycteroperca de las costas de Venezuela (Pisces-Serranidae) Mem Soc Cienc Nat LaSalle 26 77–143
DJ Colgan A McLauchlan GDF Wilson SP Livingston GD Edgecombe J Macaraenas G Casis MR Gray (1998) ArticleTitleHistone III and U2 snRNA DNA sequences and arthropod evolution Aust J Zool 46 419–437 Occurrence Handle10.1071/ZO98048
MT Craig DJ Pondella SuffixII JC Hafner JPC Franck (2001) ArticleTitleOn the status of the serranid fish genus Epinephelus: evidence for paraphyly based on 16S rDNA sequences Mol Phylogenet Evol 19 121–130 Occurrence Handle11286497 Occurrence Handle10.1006/mpev.2000.0913 Occurrence Handle1:CAS:528:DC%2BD3MXitlOjtbs%3D
MT Craig PA Hastings DJ Pondella SuffixII (2004) ArticleTitleSpeciation in the Central American Seaway: the importance of taxon sampling in the identification of geminate species pairs J Biogeogr 31 1085–1091 Occurrence Handle10.1111/j.1365-2699.2004.01035.x
Craig MT, Bartsch P, Wirtz P, Heemstra PC (2007) Redescription and revalidation of Alphestes afer (Bloch 1793) as an amphi-Atlantic grouper species (Perciformes; Serranidae). Cybium (in press)
A Dettai G Lecointre (2004) ArticleTitleIn search of the notothenioid (Teleostei) relatives Antarct Sci 16 71–85 Occurrence Handle10.1017/S095410200400183X
A Dettai G Lecointre (2005) ArticleTitleFurther support for the clades obtained by multiple molecular phylogenies in the acanthomorph bush C R Biol 328 674–689 Occurrence Handle15992750 Occurrence Handle10.1016/j.crvi.2005.04.002 Occurrence Handle1:CAS:528:DC%2BD2MXlvVWkuro%3D
K Dolphin R Belshaw CDL Orme DLJ Quicke (2000) ArticleTitleNoise and incongruence: interpreting results of the incongruence length difference test Mol Phylogenet Evol 17 401–406 Occurrence Handle11133194 Occurrence Handle10.1006/mpev.2000.0845 Occurrence Handle1:CAS:528:DC%2BD3cXptVygurY%3D
PL Forey CJ Humphries IL Kitching RW Scotland DJ Siebert DM Williams (1992) Cladistics: a practical course in systematics Clarendon Press Oxford
WA Gosline (1966) ArticleTitleThe limits of the fish family Serranidae, with notes on other lower percoids Proc Calif Acad Sci 33 91–111
PH Greenwood (1977) ArticleTitleA review of the family Centropomidae (Pisces, Perciformes): an appendix Bull Br Mus (Nat Hist) Zool 31 297–301
PH Greenwood DE Rosen SH Weitzman GE Myers (1966) ArticleTitlePhyletic studies of teleostean fishes, with a provisional classification of living forms Bull Am Mus Nat Hist 131 339–455
PC Heemstra (1991) ArticleTitleA taxonomic revision of the eastern Atlantic groupers (Pisces: Serranidae) Bol Mus Munic Funchal 43 5–71
Heemstra PC, Heemstra E (2004) Coastal fishes of southern Africa. South African Institute for Aquatic Biodiversity and National Inquiry Service Centre, Grahamstown, South Africa
PC Heemstra JE Randall (1986) Serranidae MM Smith PC Heemstra (Eds) Smith's sea fishes Macmillan South Africa Johannesburg
Heemstra PC, Randall JE (1993) Groupers of the world. FAO Fisheries synopsis no. 125, vol 16. FAO, Rome
H Imamura M Yabe (2002) ArticleTitleDemise of the Scopaeniformes (Actinoperygii: Percomorpha): an alternative phylogenetic hypothesis Bull Fac Fish Hokkaido Univ 53 107–128
GD Johnson (1983) ArticleTitle Niphon spinosus: a primitive epinepheline serranid, with comments on the monophyly and intrarelationships of the Serranidae Copeia 1983 777–787 Occurrence Handle10.2307/1444346
GD Johnson (1988) ArticleTitleNiphon spinosus, a primitive epinepheline serranid: corroborative evidence from the larvae Jpn J Ichthyol 35 7–18
GD Johnson P Keener (1984) ArticleTitleAid to identification of American grouper larvae Bull Mar Sci 34 106–134
DS Jordan (1908) ArticleTitleThe law of geminate species Am Nat 42 73–80 Occurrence Handle10.1086/278905
DS Jordan (1923) ArticleTitleA classification of fishes including families and genera as far as known Stanford Univ Publ Univ Ser Biol Sci 2 77–243
DS Jordan CH Eigenmann (1890) ArticleTitleA review of the genera and species of Serranidae found in the waters of America and Europe Bull US Fish Com VIII IssueID1888 329–441
M Katayama (1959) ArticleTitleStudies on the serranid fishes of Japan (1) Bull Fac Educ Yamaguchi Univ 8 103–180
M Katayama (1974) ArticleTitleSerranid fishes of the Okinawa Islands (II) Bull Fac Educ Yamaguchi Univ 24 99–112
AW Kendall SuffixJr (1976) ArticleTitlePredorsal and associated bones in serranid and grammistid fishes Bull Mar Sci 26 585–592
AW Kendall SuffixJr (1979) ArticleTitleMorphological comparisons of North American seabass larvae (Pisces: Serranidae:) NOAA Tech Rep Circ 428 1–50
AW Kendall SuffixJr (1984) Serranidae: development and relationships HG Moser WJ Richards DM Cohen MP Fahay AW Kendall SuffixJr SL Richardson (Eds) Ontogeny and systematics of fishes. Special publication 1 American Society of Ichthyologists and Herpetologists Lawrence, KS 499–510
AW Kendall SuffixJr MP Fahay (1979) ArticleTitleLarvae of the serranid fish Gonioplectrus hispanus with comments on its relationships Bull Mar Sci 29 117–121
JM Leis (1986) ArticleTitleLarval development in four species of Indo-Pacific coral trout Plectropomus (Pisces, Serranidae: Epinephelinae) with an analysis of relationships of the genus Bull Mar Sci 38 525–552
AE Leviton RH Gibbs SuffixJr E Heal CE Dawson (1985) ArticleTitleStandards in herpetology and ichthyology. Part I. Standard symbolic codes for institutional resource collections in herpetology and ichthyology Copeia 1985 802–832
Maddison DR, Maddison WP (1997) MacClade V. 4.06. Sinauer Associates, Sunderland, MA
T Maggio F Andaloro F Hemida M Arculeo (2005) ArticleTitleA molecular analysis of some eastern Atlantic grouper from the Epinephelus and Mycteroperca genus J Exp Mar Biol Ecol 321 83–92 Occurrence Handle10.1016/j.jembe.2005.01.004 Occurrence Handle1:CAS:528:DC%2BD2MXltVejtrs%3D
McCully HH (1961) The comparative anatomy of the scales of serranid fishes. PhD dissertation. Stanford University, Palo Alto, CA
MR Meisler (1987) Limits and relationships of serranine seabasses, with revisions of Serranus and Mentiperca (Pisces: Serranidae). PhD dissertation University of Southern California Los Angeles
JS Nelson (1994) Fishes of the world EditionNumber3rd edn Wiley New York
KC Nixon (1999) ArticleTitleThe parsimony ratchet, a new method for rapid parsimony analysis Cladistics 15 407–414 Occurrence Handle10.1111/j.1096-0031.1999.tb00277.x
JR Norman (1966) A draft synopsis of the orders, families and genera of recent fishes and fish-like vertebrates British Museum (Natural History) London
Y Oshima K Shiomi Y Hashimoto (1974) ArticleTitleComparison of grammistin from four species of grammistid fishes Bull Jpn Soc Sci Fish 40 223–230 Occurrence Handle1:CAS:528:DyaE2cXksVOmu7c%3D
O Otero (2005) ArticleTitleAnatomy, systematics and phylogeny of both recent and fossil latid fishes (Teleostei, Perciformes, Latidae) Zool J Linn Soc 141 81–133 Occurrence Handle10.1111/j.1096-3642.2004.00111.x
S Palumbi (1996) Nucleic acids II: The polymerase chain reaction DM Hillis C Moritz BK Maple (Eds) Molecular systematics Sinauer Sunderland 205–248
D Posada KA Crandall (1998) ArticleTitleModeltest: testing the model of DNA substitution Bioinformatics 14 817–818 Occurrence Handle9918953 Occurrence Handle10.1093/bioinformatics/14.9.817 Occurrence Handle1:CAS:528:DyaK1MXktlCltw%3D%3D
JE Randall (1964) ArticleTitleNotes on the groupers of Tahiti, with description of a new serranid fish genus Pac Sci 18 281–296
JE Randall K Aida T Hibiya N Mitsuura H Kamiya Y Hashimoto (1971) ArticleTitleGrammistin, the skin toxin of soapfishes and its significance in the classification of the Grammistidae Publ Seto Mar Biol Lab 19 157–190
DR Rosen (1979) ArticleTitleFishes from the uplands and intermontane basins of Guatemala: revisionary studies and comparative geography Bull Am Mus Nat Hist 162 269–375
RH Rosenblatt BJ Zahuranec (1967) ArticleTitleThe Eastern Pacific groupers of the genus Mycteroperca, including a new species Cal Fish Game 53 228–245
K Shiomi T Igarashi H Yokota Y Nagashima M Ishida (2000) ArticleTitleIsolation and structures of grammistins, peptide toxins from the skin secretion of the soapfish Grammistes sexlineatus Toxicon 38 91–103 Occurrence Handle10669014 Occurrence Handle10.1016/S0041-0101(99)00136-1 Occurrence Handle1:CAS:528:DyaK1MXns1GlurY%3D
CG Sibley (1957) ArticleTitleThe evolutionary and taxonomic significance of sexual dimorphism and hybridization of birds Condor 59 161–191 Occurrence Handle10.2307/1364722
DS Sikes PO Lewis (2001) Beta software, V. 1. PAUPRat: PAUP* implementation of the parsimony ratchet Dept. of Ecology and Evolutionary Biology, University of Connecticut Storrs, CT
CL Smith (1954) ArticleTitleFour rare serraniform fishes from east Africa Ann Mag Nat Hist (Ser 12) 7 925–933
CL Smith (1966) ArticleTitle Menephorus Poey, a serranid genus based on two hybrids of Cephalopholis fulva and Paranthias furcifer, with comments on the systematic placement of Paranthias Am Mus Novit 2276 1–11
CL Smith (1971) ArticleTitleA revision of the American groupers: Epinephelus and allied genera Bull Am Mus Nat Hist 146 1–241
WL Smith WC Wheeler (2004) ArticleTitlePolyphyly of the mail-cheeked fishes (Teleostei: Scorpaeniformes): evidence from mitochondrial and nuclear sequence data Mol Phylogenet Evol 32 627–646 Occurrence Handle15223043 Occurrence Handle10.1016/j.ympev.2004.02.006 Occurrence Handle1:CAS:528:DC%2BD2cXlt1eks70%3D
WF Smith-Vaniz GD Johnson JE Randall (1988) ArticleTitleRedescription of Gracila albomarginata (Fowler and Bean) and Cephalopholis polleni (Bleeker) with comments on the generic limits of selected Indo-Pacific groupers (Pisces: Serranidae: Epinephelinae) Proc Acad Nat Sci Phila 140 1–23
JT Streelman SA Karl (1997) ArticleTitleReconstructing labroid evolution with single-copy nuclear DNA Proc R Soc Lond 264 1011–1020 Occurrence Handle10.1098/rspb.1997.0140 Occurrence Handle1:CAS:528:DyaK2sXls1Wiu7w%3D
N Sugiyama M Araki M Ishida Y Nagashima K Shiomi (2005) ArticleTitleFurther isolation and characterization of grammistins from the skin secretion of the soapfish Grammistes sexlineatus Toxicon 45 595–601 Occurrence Handle15777955 Occurrence Handle10.1016/j.toxicon.2004.12.021 Occurrence Handle1:CAS:528:DC%2BD2MXitlShtbY%3D
JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res 25 4876–4882 Occurrence Handle9396791 Occurrence Handle10.1093/nar/25.24.4876 Occurrence Handle1:CAS:528:DyaK1cXntFyntQ%3D%3D
RA Vos (2003) ArticleTitleAccelerated likelihood surface exploration: the likelihood ratchet Syst Biol 52 368–373 Occurrence Handle12775525 Occurrence Handle1:STN:280:DC%2BD3s3lt1Cruw%3D%3D
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Craig, M., Hastings, P. A molecular phylogeny of the groupers of the subfamily Epinephelinae (Serranidae) with a revised classification of the Epinephelini. Ichthyol Res 54, 1–17 (2007). https://doi.org/10.1007/s10228-006-0367-x
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DOI: https://doi.org/10.1007/s10228-006-0367-x