Abstract
It is generally accepted that methicillin-resistant Staphylococcus aureus (MRSA) is also resistant to aminoglycoside antibiotics. We investigated trends of gentamicin and arbekacin susceptibilities and the prevalence of the genes encoding aminoglycoside-modifying enzymes (AMEs) for a total of 218 strains of MRSA isolated from blood specimens obtained from 1978 through 2002 in one hospital. The minimum inhibitory concentrations of gentamicin at which 50% of the strains were inhibited (MIC50) were ≥128 and 32 µg/ml for isolates obtained from 1978 to 1984 and from 1985 to 1989, respectively, and 0.5 µg/ml for isolates obtained from 1990 to 2002. The MIC90 of gentamicin was consistently ≥128 µg/ml. Investigation of the occurrence of AME revealed that the MIC50 of gentamicin was highly correlated with the presence of aac(6′)/aph(2″) encoding aminoglycoside acetyl/phosphotransferase. The MIC50 of arbekacin was 2 µg/ml for strains isolated in 1978–1984 and ≤0.5 µg/ml for strains isolated from 1985 to 2002. The MIC90 of arbekacin was 8 µg/ml for the strains isolated in 1978–1989 and 1 to 2 µg/ml for strains isolated in 1990–2002. Though it has been established that AAC(6′)/APH(2″) modifies arbekacin, the trend of arbekacin resistance was not necessarily consistent with the presence of this enzyme. However, the prevalence of both aac(6′)/aph(2″) and aph(3′)-III in the strains isolated from 1978 through 2002 was correlated with the MIC90 values of arbekacin. Thus, it is most likely that APH(3′)-III, in addition to AAC(6′)/APH(2″), is somehow involved in arbekacin resistance in S. aureus. Our results imply that gentamicin- and arbekacin-resistant MRSAs have consistently decreased for the past 25 years and that this finding is, most likely, attributable to the declining prevalence of genes encoding for AMEs.
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AL Panlilio DH Culver RP Gaynes S Banerjee TS Henderson JS Tolson et al. (1992) ArticleTitleMethicillin-resistant Staphylococcus aureus in U.S. hospitals, 1975–1991 Infect Control Hosp Epidemiol 13 582–6 Occurrence Handle1469266 Occurrence Handle1:STN:280:DyaK3s7gslKgtA%3D%3D Occurrence Handle10.1086/646432
A Sunada S Asari (2003) ArticleTitleReport of questionnaire survey for methicillin-resistant Staphylococcus aureus and penicillin-resistant Streptococcus pneumoniae between 1998 and 2000 in the Kinki district Kansenshogaku Zasshi 77 331–9 Occurrence Handle12806929
N Noguchi H Nakaminami S Nishijima I Kurokawa H So M Sasatsu (2006) ArticleTitleAntimicrobial agent of susceptibilities and antiseptic resistance gene distribution among methicillin-resistant Staphylococcus aureus isolates from patients with impetigo and staphylococcal scalded skin syndrome J Clin Microbiol 44 2119–25 Occurrence Handle16757607 Occurrence Handle10.1128/JCM.02690-05 Occurrence Handle1:CAS:528:DC%2BD28XmvFKgsb4%3D
HS Sader JM Streit TR Fritsche RN Jones (2006) ArticleTitleAntimicrobial susceptibility of gram-positive bacteria isolated from European medical centres: results of the Daptomycin Surveillance Programme (2002–2004) Clin Microbiol Infect 12 844–52 Occurrence Handle16882289 Occurrence Handle10.1111/j.1469-0691.2006.01550.x Occurrence Handle1:CAS:528:DC%2BD28XhtVeht7bL
Jones ME, Draghi DC, Thornsberry C, Karlowsky JA, Sahm DF, Wenzel RP. Emerging resistance among bacterial pathogens in the intensive care unit – a European and North American surveillance study (2000–2002). Ann Clin Microbiol Antimicrob 2004;3:14th pp 1–11
K Hiramatsu H Hanaki T Ino K Yabuta T Oguri FC Tenover (1997) ArticleTitleMethicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility J Antimicrob Chemother 40 135–6 Occurrence Handle9249217 Occurrence Handle10.1093/jac/40.1.135 Occurrence Handle1:CAS:528:DyaK2sXltVCit7w%3D
SK Fridkin J Hageman LK McDougal J Mohammed WR Jarvis TM Perl et al. (2003) ArticleTitleEpidemiological and microbiological characterization of infections caused by Staphylococcus aureus with reduced susceptibility to vancomycin, United States, 1997–2001 Clin Infect Dis 36 429–39 Occurrence Handle12567300 Occurrence Handle10.1086/346207
InstitutionalAuthorNameCenters for Disease Control and Prevention (2002) ArticleTitlePublic Health Dispatch: vancomycin-resistant Staphylococcus aureus – Pennsylvania, 2002 Morb Mortal Wkly Rep 51 902–3
InstitutionalAuthorNameCenters for Disease Control and Prevention (2002) ArticleTitle Staphylococcus aureus resistant to vancomycin – United States, 2002 Morb Mortal Wkly Rep 51 565–7
InstitutionalAuthorNameCenters for Disease Control and Prevention (2004) ArticleTitleVancomycin-resistant Staphylococcus aureus – New York, 2004 Morb Mortal Wkly Rep 53 322–3
HB Kim HC Jang HJ Nam YS Lee BS Kim WB Park et al. (2004) ArticleTitleIn vitro activities of 28 antimicrobial agents against Staphylococcus aureus isolates from tertiary-care hospitals in Korea: a nationwide survey Antimicrob Agents Chemother 48 1124–7 Occurrence Handle15047511 Occurrence Handle10.1128/AAC.48.4.1124-1127.2004 Occurrence Handle1:CAS:528:DC%2BD2cXjtVKrurY%3D
T Ida R Okamoto C Shimauchi T Okubo A Kuga M Inoue (2001) ArticleTitleIdentification of AMEs by susceptibility testing: epidemiology of methicillin-resistant Staphylococcus aureus in Japan J Clin Microbiol 39 3115–21 Occurrence Handle11526138 Occurrence Handle10.1128/JCM.39.9.3115-3121.2001 Occurrence Handle1:CAS:528:DC%2BD3MXnt1Wgur0%3D
B Llano-Sotelo EF Azucena LP Kotra S Mobashery CS Chow (2002) ArticleTitleAminoglycosides modified by resistance enzymes display diminished binding to the bacterial ribosomal aminoacyl-tRNA site Chem Biol 9 455–63 Occurrence Handle11983334 Occurrence Handle10.1016/S1074-5521(02)00125-4 Occurrence Handle1:CAS:528:DC%2BD38Xjt1Cqtbg%3D
LP Kotra J Haddad S Mobashery (2000) ArticleTitleAminoglycosides: perspec-tives on mechanisms of action and resistance and strategies to counter resistance Antimicrob Agents Chemother 44 3249–56 Occurrence Handle11083623 Occurrence Handle10.1128/AAC.44.12.3249-3256.2000 Occurrence Handle1:CAS:528:DC%2BD3MXhs1Kisbc%3D
N Tanaka K Matsunaga A Hirata Y Matsuhisa T Nishimura (1983) ArticleTitleMechanism of action of habekacin, a novel amino acid-containing aminoglycoside antibiotic Antimicrob Agents Chemother 24 797–802 Occurrence Handle6362557 Occurrence Handle1:CAS:528:DyaL2cXjt1SqsQ%3D%3D
K Ubukata N Yamashita A Gotoh M Konno (1984) ArticleTitlePurification and characterization of AMEs from Staphylococcus aureus and Staphylococcus epidermidis Antimicrob Agents Chemother 25 754–9 Occurrence Handle6331299 Occurrence Handle1:CAS:528:DyaL2cXltVKiur0%3D
M Tabata M Shimizu M Araake H Ogawa (2003) ArticleTitleRelationship between arbekacin-susceptibility and aminoglycoside-resistant gene of methicillin-resistant Staphylococcus aureus (MRSA) Jpn J Antibiot 56 36–43 Occurrence Handle12723397 Occurrence Handle1:CAS:528:DC%2BD3sXit1Krt78%3D
K Ishino J Ishikawa Y Ikeda K Hotta (2004) ArticleTitleCharacterization of a bifunctional aminoglycoside-modifying enzyme with novel substrate specificity and its gene from a clinical isolate of methicillin-resistant Staphylococcus aureus with high arbekacin resistance J Antibiot 57 679–86 Occurrence Handle15638329 Occurrence Handle1:CAS:528:DC%2BD2cXptlOgtro%3D
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: 15th informational supplement. CLSI/NCCLS document M100-S15. Clinical and Laboratory Standards Institute, Wayne, PA: 2005
A Saito (1995) ArticleTitleClinical breakpoints for antimicrobial agents in pulmonary infections and sepsis: Report of The Committee for Japanese Standards for Antimicrobial Susceptibility Testing for Bacteria J Infect Chemother 1 83–8 Occurrence Handle10.1007/BF02347736
N Tsuchizaki J Ishikawa K Hotta (2000) ArticleTitleColony PCR for rapid detection of antibiotic resistance genes in MRSA and enterococci Jpn J Antibiot 53 422–9 Occurrence Handle10955238 Occurrence Handle1:CAS:528:DC%2BD3cXkslSltbg%3D
K Hotta J Ishikawa R Ishii F Saitoh K Kira Y Arakawa et al. (1999) ArticleTitleNecessity and usefulness of detection by PCR of mecA and aac(6′)/aph(2″) genes for identification of arbekacin-resistant MRSA Jpn J Antibiot 52 525–32 Occurrence Handle10587877 Occurrence Handle1:CAS:528:DyaK1MXlsl2ksL4%3D
R Vanhoof C Godard J Content HJ Nyssen E Hannecart-Pokorni InstitutionalAuthorNamethe Belgian Study Group of Hospital Infections (GDEPIH/GOSPIZ) (1994) ArticleTitleDetection by polymerase chain reaction of genes encoding AMEs in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types J Med Microbiol 41 282–90 Occurrence Handle7932622 Occurrence Handle1:CAS:528:DyaK2MXit1altbo%3D Occurrence Handle10.1099/00222615-41-4-282
N Tsuchizaki K Ishino F Saito J Ishikawa M Nakajima K Hotta (2006) ArticleTitleTrends of arbekacin-resistant MRSA strains in Japanese hospitals (1979 to 2000) J Antibiot 59 229–33 Occurrence Handle16830890 Occurrence Handle1:CAS:528:DC%2BD28XkvFSgtrg%3D Occurrence Handle10.1038/ja.2006.32
S Kondo K Hotta (1999) ArticleTitleSemisynthetic aminoglycoside antibiotics: development and enzymatic modifications J Infect Chemother 5 1–9 Occurrence Handle11810483 Occurrence Handle10.1007/s101560050001 Occurrence Handle1:CAS:528:DyaK1MXislKiu7o%3D
N Ardic B Sareyyupoglu M Ozyurt T Haznedaroglu U Ilga (2006) ArticleTitleInvestigation of aminoglycoside modifying enzyme genes in methicillin-resistant staphylococci Microbiol Res 161 49–54 Occurrence Handle16338590 Occurrence Handle10.1016/j.micres.2005.05.002 Occurrence Handle1:CAS:528:DC%2BD28XhslOit74%3D
F Schmitz AC Fluit M Gondolf R Beyrau E Lindenlauf J Verhoef et al. (1999) ArticleTitleThe prevalence of aminoglycoside resistance and corresponding genes in clinical isolates of staphylococci from 19 European hospitals J Antimicrob Chemother 43 253–9 Occurrence Handle11252331 Occurrence Handle10.1093/jac/43.2.253 Occurrence Handle1:CAS:528:DyaK1MXhsFyms7s%3D
SM Choi S Kim C Kim D Lee J Choi J Yoo et al. (2003) ArticleTitleMultiplex PCR for the detection of genes encoding aminoglycoside modifying enzymes and methicillin resistance among Staphylococcus species J Korean Med Sci 18 631–6 Occurrence Handle14555812 Occurrence Handle1:CAS:528:DC%2BD3sXptF2gu78%3D
H Matsuo M Kobayashi T Kumagai M Kuwabara M Sugiyama (2003) ArticleTitleMolecular mechanism for the enhancement of arbekacin resistance in a methicillin-resistant Staphylococcus aureus FEBS Lett 546 401–6 Occurrence Handle12832077 Occurrence Handle10.1016/S0014-5793(03)00644-6 Occurrence Handle1:CAS:528:DC%2BD3sXkvVKjtb8%3D
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Barada, K., Hanaki, H., Ikeda, S. et al. Trends in the gentamicin and arbekacin susceptibility of methicillin-resistant Staphylococcus aureus and the genes encoding aminoglycoside-modifying enzymes. J Infect Chemother 13, 74–78 (2007). https://doi.org/10.1007/s10156-006-0497-0
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DOI: https://doi.org/10.1007/s10156-006-0497-0