Abstract
Here we describe alterations in the cinnamate/monolignol pathway in three transgenic aspen lines: one with downregulated expression of 4-coumarate:CoA ligase (4CL), one with upregulated expression of coniferaldehyde 5-hydroxylase (CAld5H), and a 4CL downregulated/CAld5H upregulated line. Compared with the wild type, the 4CL downregulated line showed significantly increased levels of p-hydroxycinnamic acids such as p-coumaric, ferulic, and sinapic acids. In contrast, the CAld5H upregulated line had increased content of p-coumaryl and 5-hydroxyconiferyl alcohols. In the 4CL downregulated line, it was likely that most hydroxycinnamic acids were glycosylated. These results strongly suggest that the downregulation of 4CL and upregulation of CAld5H disrupt the metabolic flow through the cinnamate/monolignol pathway and thus alter the amount and structure of its final product, lignin.
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Li L, Zhou Y, Cheng X, Sun J, Marta JM, Ralph J, Chiang VL (2003) Combinatorial modification of multiple lignin traits in trees through multigene cotransformation. Proc Natl Acad Sci U S A 100:4939–4944
Hu WJ, Lung J, Harding SA, Popko JL, Ralph J, Stokke DD, Tsai CJ, Chiang VL (1999) Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees. Nat Biotechnol 17:808–812
Sakakibara N, Nakatsubo T. Suzuki S, Shibata D, Shimada M, Umezawa T (2007) Metabolic analysis of the cinnamate/monolignol pathway in Carthamus tinctorius seeds by a stable-isotopedilution method. Org Biomol Chem 5:802–815
Suzuki S, Nakatsubo T, Umezawa T, Shimada M (2002) First in vitro norlignan formation with Asparagus officinalis enzyme preparation. Chem Commun 2002:1088–1089
Schoental R (1973) Carcinogenicity of wood shavings. Lab Anim 7:47–49
Meyermans H, Morreel K, Lapierre C, Pollet B, De Bruyn A, Busson R, Herdewijn P, Devreeese B, Van Beeumen J, Marita JM, Ralph J, Chen C, Burggraeve B, Van Montagu M, Messens E, Boerjan W (2000) Modifications in lignin and accumulation of phenolic glycosides in poplar xylem upon downregulation of caffeoyl coenzyme A O-methyltransferase, an enzyme involved in lignin biosynthesis. J Biol Chem 275:36899–36909
Chiang VL (2006) Monolignol biosynthesis and genetic engineering of lignin in trees: a review. Environ Chem Lett 4:143–146
Osakabe K, Tsao CC, Li L, Popko JL, Umezawa T, Carraway DT, Smeltzer RH, Joshi CP, Chiang VL (1999) Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms. Proc Natl Acad Sci U S A 96:8955–8960
Li L, Popko JL, Umezawa T, Chiang VL (2000) 5-Hydroxyconiferyl aldehyde modulates enzymatic methylation for syringyl monolignol formation, a new view of monolignol biosynthesis in angiosperms. J Biol Chem 275:6537–6545
Li L, Chen XF, Leshkevich J, Umezawa T, Harding SA, Chiang VL (2001) The last step of syringyl monolignol biosynthesis in angiosperms is regulated by a novel gene encoding sinapyl alcohol dehydrogenase. Plant Cell 13:1567–1585
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Suzuki, S., Sakakibara, N., Li, L. et al. Profiling of phenylpropanoid monomers in developing xylem tissue of transgenic aspen (Populus tremuloides). J Wood Sci 56, 71–76 (2010). https://doi.org/10.1007/s10086-009-1059-8
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DOI: https://doi.org/10.1007/s10086-009-1059-8