Abstract
In present study, the effect of crude and commercial enzyme treatment on pineapple (Ananas comosus) juice yield, clarity, and viscosity was studied. A central composite face centered design was used to establish the optimum conditions for enzymatic hydrolysis of pineapple using crude enzyme. The optimized crude enzyme treatment conditions were incubation temperature 47°C, incubation time 446 min, and enzyme concentration 0.14 mL/50 g of pulp. The conditions for the commercial enzyme treatment of the same variety of pineapple to improve the juice recovery and quality were also optimized. The data showed that the crude enzyme was competitive to the commercial enzymes for the improvement of juice recovery and quality from pineapple. The comparison was done under optimized conditions using principal component analysis. As viscosity was the most uncorrelated variable, hence it can be used as a variable to study variance among samples treated by crude and commercial enzymes.
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References
Thomas SCL. Vegetable and Fruits Nutritional and Therapeutic Values. CRC Press Taylor and Francis Group, New York, NY, USA. p. 188 (2008)
Uhlig H. Industrial Enzymes and Their Applications. John Wiley & Sons, New York, NY, USA. p. 435 (1998)
Grassin C, Fauquembergue P. Fruit juices. pp. 226–264. In: Industrial Enzymology. Godfrey T, West S (eds). MacMillan Press, London, UK (1996)
Galante YM, De Conti A, Monteverdi R. Application of Trichoderma enzymes in textile industry. Vol. II, pp. 311–326. In: Trichoderma & Gliocladium-Enzymes, Biological Control and Commercial Applications. Harman GF, Kubicek CP (eds). Taylor & Francis, London, UK (1998)
Sreenath HK, Sudarshanakrishna KR, Santhanam K. Improvement of juice recovery from pineapple pulp/residue using cellulases and pectinases. J. Ferment. Bioeng. 78: 486–488 (1994)
Pal A, Khanum F. Efficacy of xylanase purified from Aspergillus niger DFR-5 alone and in combination with pectinase and cellulase to improve yield and clarity of pineapple juice. J. Food Sci. Technol. 48: 560–568 (2011)
Kumar S, Sharma HK, Sarkar BC. Effect of substrate and fermentation conditions on pectinase and cellulase production by Aspergillus niger NCIM 548 in submerged (SmF) and solid state fermentation (SSF). Food Sci. Biotechnol. 20: 1279–1298 (2011)
Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Biochem. 31: 426–428 (1959)
Montgomery DC. Design and Analysis of Experiments. John Wiley & Sons, Inc., New York, NY, USA p. 427 (2001)
Kaur S, Sarkar BC, Sharma HK, Singh C. Optimization of enzymatic hydrolysis pretreatment conditions for enhanced juice recovery from guava fruit using response surface methodology. Food Bioprocess Tech. 2: 96–100 (2009)
Rai P, Majumdar GC, DasGupta S, De S. Optimizing pectinase usage in pretreatment of mosambi juice for clarification by response surface methodology. J. Food Eng. 64: 397–403 (2004)
Sreenath HK, Santhanam K. The use of commercial enzymes in white grape juice clarification. J. Ferment. Bioeng. 73: 241–243 (1992)
Pandey H, Sharma HK, Chauhan RC, Sarkar BC, Bera MB. Experiments in Food Process Engineering. CBS Publishers and Distributers, New Delhi, India. pp. 60–61 (2004)
Kashyap DR, Vohra PK, Chopra S, Tewari R. Applications of pectinases in the commercial sector: A review. Bioresource Technol. 77: 215–227 (2001)
Lee WC, Yusof S, Hamid NSA, Baharin BS. Optimizing conditions for enzymatic clarification of banana juice using response surface methodology. J. Food Eng. 73: 55–63 (2006)
Vaillant F, Millan P, O’Brain G, Douvier M, Reynes M. Crosflow microfiltration of passion fruit juice after partial enzymatic liquefaction. J. Food Eng. 42: 215–224 (1999)
Sin HN, Yusof S, Sheikh Abdul Hamid N, Abd. Rahman R. Optimization of enzymatic clarification of sapodilla juice using response surface methodology. J. Food Eng. 73: 313–319 (2006)
Singh A, Kumar S, Sharma HK. Effect of enzymatic hydrolysis the juice yield from bael fruit (Aegle marmelos Correa) pulp. Am. J. Food Technol. 7: 62–72 (2012)
Demir NJ, Acar K, Sario LU, Mutlu M. The use of commercial pectinase in fruit juice industry. Part 3: Immobilized pectinase for mash treatment. J. Food Eng. 47: 275–280 (2000)
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Kumar, S., Sharma, H.K. Comparative effect of crude and commercial enzyme on the juice recovery from pineapple (Ananas comosus) using principal component analysis (PCA). Food Sci Biotechnol 21, 959–967 (2012). https://doi.org/10.1007/s10068-012-0126-x
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DOI: https://doi.org/10.1007/s10068-012-0126-x