Abstract
Anti-adipogenesis activity of methanol extract from hot pepper (Capsicum annuum L.) seeds (PSE) was evaluated. Exposure to PSE (25–200 μg/mL) for a 72 hr incubation period did not alter cell viability compared to the control. The adipocyte treated with PSE at the concentration of 200, 100, and 50 μg/mL showed decreased color intensity as compared to the control suggesting decreased lipid accumulation in the adipocyte. Moreover, the glycerol-3-phosphate dehydrogenase activity was significantly reduced. The expression of C/EBPβ, C/EBPα, and PPARγ, important adipogenic transcription factors, was significantly reduced when PSE was added to cells from day 0 to 6 as compared with the control group. The results suggest that PSE has significant health-promoting effects, having excellent anti-obesity activities.
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References
Kopelman PG. Obesity as a medical problem. Nature 404: 635–643 (2000)
Rayalam S, Della-Fera MA, Baile CA. Phytochemicals and regulation of the adipocyte life cycle. J. Nutr. Biochem. 19: 717–726 (2008)
Gregoire FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiol. Rev. 78: 783–809 (1998)
Gregoire FM. Adipocyte differentiation: From fibroblast to endocrine cell. Exp. Biol. Med. 226: 997–1002 (2001)
Bagchi D, Preuss HG. Obesity: Epidemiology, Pathophysiology, and Prevention. CRC Press. Boca Raton, FL, USA. pp. 233–238 (2007)
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55–63 (1983)
Wise LS, Green H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J. Biol. Chem. 254: 273–275 (1979)
Roncari DA, Lau DC, Kindler S. Exaggerated replication in culture of adipocyte precursors from massively obese persons. Metabolism 30: 425–427 (1981)
Sorisky A, Magun R, Gagnon AM. Adipose cell apoptosis: Death in the energy depot. Int. J. Obes. Relat. Metab. Disord. 24: S3–S7 (2000)
Ntambi JM, Kim YC. Adipocyte differentiation and gene expression. J. Nutr. 130: 3122S–3126S (2000)
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Jeon, G., Choi, Y., Lee, SM. et al. Anti-obesity activity of methanol extract from hot pepper (Capsicum annuum L.) seeds in 3T3-L1 adipocyte. Food Sci Biotechnol 19, 1123–1127 (2010). https://doi.org/10.1007/s10068-010-0160-5
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DOI: https://doi.org/10.1007/s10068-010-0160-5