Abstract
Background
The consumption of antioxidant nutrients may influence the development and progression of osteoarthritis (OA). To determine the association between serum antioxidants and radiographic knee osteoarthritis, we undertook a cross-sectional investigation in a community-based study in Japan.
Methods
A total of 562 subjects (224 male, 338 female) ≥40 years of age were enrolled in the Comprehensive Health Examination Program (CHEP, Yakumo Study) from 2003 to 2005. Subjects were categorized to the OA group (n = 140) if either knee was graded as Kellgren-Lawrence (K-L) grade ≥2. The no-OA group was defined as showing radiographic findings of K-L 0 or 1 in either knee (n = 422). The serum levels of retinol, β-/γ-tocopherols, α-tocopherol, zeaxanthin/lutein, canthaxanthin, cryptoxanthin, lycopene, α-carotene, and β-carotene were measured by high-performance liquid chromatography. The values of these antioxidants were divided into tertiles, and a logistic regression analysis was performed to analyze the association between them and radiographic knee OA, adjusting for potential confounders.
Results
Logistic regression analysis showed that compared to the lowest tertile of β-/γ-tocopherols the adjusted odds ratio (OR) was 0.52 [95% confidence interval (CI) 0.29–0.93] in the highest tertile; it also indicated a linear trend across tertiles. Furthermore, the adjusted OR was significantly decreased only in the middle tertile of α-tocopherol (OR 0.51, 95% CI 0.29–0.90). We reevaluated any independent association for these tocopherols after adjustment by entering them into the model simultaneously. The significance of β-/γ-tocopherols was maintained. In contrast, no associations were found with any carotenoids or retinol.
Conclusions
High serum values of β-/γ-tocopherols were found to be significantly associated with a low OR for radiographic knee osteoarthritis. The decreasing risk with a high serum value of β-/γ-tocopherols may support the possible protective effects against knee OA.
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References
Felson DT, Naimark A, Anderson J, Kazis L, Castelli W, Meenan RF. The prevalence of knee osteoarthritis in the elderly: the Framingham Osteoarthritis Study. Arthritis Rheum 1987;30:914–918.
Sylvester PW. Vitamin E and apoptosis. Vitam Horm 2007;76:329–356.
Vainio H, Rautalahti M. An international evaluation of the cancer preventive potential of carotenoids. Cancer Epidemiol Biomarkers Prev 1998;7:725–728.
Peto R, Doll R, Buckley JD, Sporn MB. Can dietary beta-carotene materially reduce human cancer rates? Nature 1981;290:201–208.
Ito Y, Kurata M, Suzuki K, Hamajima N, Hishida H, Aoki K. Cardiovascular disease mortality and serum carotenoid levels: a Japanese population-based follow-up study. J Epidemiol 2006;16:154–160.
Ikeda T, Mabuchi A, Fukuda A, Kawakami A, Ryo Y, Yamamoto S, et al. Association analysis of single nucleotide polymorphisms in cartilage-specific collagen genes with knee and hip osteoarthritis in the Japanese population. J Bone Miner Res 2002;17:1290–1296.
Kanoh T, Hasegawa Y, Masui T, Yamaguchi J, Ishiguro N, Hamajima N. Interleukin-1beta gene polymorphism associated with radiographic signs of osteoarthritis of the knee. J Orthop Sci 2008;13:97–100.
Loeser RF, Carlson CS, Del Carlo M, Cole A. Detection of nitrotyrosine in aging and osteoarthritic cartilage: correlation of oxidative damage with the presence of interleukin-1beta and with chondrocyte resistance to insulin-like growth factor 1. Arthritis Rheum 2002;46:2349–2357.
Yudoh K, Nguyen T, Nakamura H, Hongo-Masuko K, Kato T, Nishioka K. Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function. Arthritis Res Ther 2005;7:R380–R391.
Schwartz ER, Oh WH, Leveille CR. Experimentally induced osteoarthritis in guinea pigs: metabolic responses in articular cartilage to developing pathology. Arthritis Rheum 1981;24:1345–1355.
Kurz B, Jost B, Schunke M. Dietary vitamins and selenium diminish the development of mechanically induced osteoarthritis and increase the expression of antioxidative enzymes in the knee joint of STR/1N mice. Osteoarthritis Cartilage 2002;10:119–126.
Wang Y, Hodge AM, Wluka AE, English DR, Giles GG, O’sullivan R, et al. Effect of antioxidants on knee cartilage and bone in healthy, middle-aged subjects: a cross-sectional study. Arthritis Res Ther 2007;9:R66.
McAlindon TE, Jacques P, Zhang Y, Hannan MT, Aliabadi P, Weissman B, et al. Do antioxidant micronutrients protect against the development and progression of knee osteoarthritis? Arthritis Rheum 1996;39:648–656.
De Roos AJ, Arab L, Renner JB, Craft N, Luta G, Helmick CG, et al. Serum carotenoids and radiographic knee osteoarthritis: the Johnston County Osteoarthritis Project. Public Health Nutr 2001;4:935–942.
Jordan JM, De Roos AJ, Renner JB, Luta G, Cohen A, Craft N, et al. A case-control study of serum tocopherol levels and the alpha- to gamma-tocopherol ratio in radiographic knee osteoarthritis: the Johnston County Osteoarthritis Project. Am J Epidemiol 2004;159:968–977.
Rosenberg TD, Paulos LE, Parker RD, Coward DB, Scott SM. The forty-five-degree posteroanterior flexion weight-bearing radiograph of the knee. J Bone Joint Surg Am 1988;70:1479–1483.
Kellgren JH, Lawrence JS. Radiological assessment of osteoarthrosis. Ann Rheum Dis 1957;16:494–502.
Ito Y, Ochiai J, Sasaki R, Suzuki S, Kusuhara Y, Morimitsu Y, et al. Serum concentrations of carotenoids, retinol, and alphatocopherol in healthy persons determined by high-performance liquid chromatography. Clin Chim Acta 1990;194:131–144.
Faure H, Preziosi P, Roussel AM, Bertrais S, Galan P, Hercberg S, et al. Factors influencing blood concentration of retinol, alpha-tocopherol, vitamin C, and beta-carotene in the French participants of the SU.VI.MAX trial. Eur J Clin Nutr 2006;60:706–717.
Cillero-Pastor B, Carames B, Lires-Dean M, Vaamonde-Garcia C, Blanco FJ, Lopez-Armada MJ. Mitochondrial dysfunction activates cyclooxygenase 2 expression in cultured normal human chondrocytes. Arthritis Rheum 2008;58:2409–2419.
Jiang Q, Christen S, Shigenaga MK, Ames BN. Gamma-tocopherol, the major form of vitamin E in the US diet, deserves more attention. Am J Clin Nutr 2001;74:714–722.
Cooney RV, Franke AA, Harwood PJ, Hatch-Pigott V, Custer LJ, Mordan LJ. Gamma-tocopherol detoxification of nitrogen dioxide: superiority to alpha-tocopherol. Proc Natl Acad Sci U S A 1993;90:1771–1775.
Jiang Q, Elson-Schwab I, Courtemanche C, Ames BN. Gammatocopherol and its major metabolite, in contrast to alpha-tocopherol, inhibit cyclooxygenase activity in macrophages and epithelial cells. Proc Natl Acad Sci U S A 2000;97:11494–11499.
Palozza P. Prooxidant actions of carotenoids in biologic systems. Nutr Rev 1998;56:257–265.
Brand C, Snaddon J, Bailey M, Cicuttini F. Vitamin E is ineffective for symptomatic relief of knee osteoarthritis: a six month double blind, randomised, placebo controlled study. Ann Rheum Dis 2001;60:946–949.
Wluka AE, Stuckey S, Brand C, Cicuttini FM. Supplementary vitamin E does not affect the loss of cartilage volume in knee osteoarthritis: a 2 year double blind randomized placebo controlled study. J Rheumatol 2002;29:2585–2591.
Winrow VR, Winyard PG, Morris CJ, Blake DR. Free radicals in inflammation: second messengers and mediators of tissue destruction. Br Med Bull 1993;49:506–522.
Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993;49:481–493.
Olmedilla B, Granado F, Southon S, Wright AJ, Blanco I, Gil-Martinez E, et al. Serum concentrations of carotenoids and vitamins A, E, and C in control subjects from five European countries. Br J Nutr 2001;85:227–238.
Sudo A, Miyamoto N, Horikawa K, Urawa M, Yamakawa T, Yamada T, et al. Prevalence and risk factors for knee osteoarthritis in elderly Japanese men and women. J Orthop Sci 2008;13:413–418.
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Seki, T., Hasegawa, Y., Yamaguchi, J. et al. Association of serum carotenoids, retinol, and tocopherols with radiographic knee osteoarthritis: possible risk factors in rural Japanese inhabitants. J Orthop Sci 15, 477–484 (2010). https://doi.org/10.1007/s00776-010-1491-z
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DOI: https://doi.org/10.1007/s00776-010-1491-z