Summary.
Cadmium had a highly toxic effect on pollen germination and tube growth, which were greatly inhibited as metal concentrations increased. Cadmium concentrations up to 10−2 M completely stopped pollen germination and pollen showed an increasing tendency to burst within 1 h. At low concentrations, the metal caused a slight stimulation of pollen germination, growth rate and tube elongation at the initial stages of tube development. Comparing the two plants studied, cadmium was more toxic for Nicotiana tabacum than for Lilium longiflorum pollen. Pollen tubes showed a range of strong morphological abnormalities, characterized by uneven or aberrant growth, including apical branching or swelling at the tip of the pollen tube. Cell wall intrusions at or near the tip were evident on the inner side, whereas a loose network formed from fibrillar material was observed on the outer layers. After prolonged cadmium exposure, round (ball-like) aggregates were embedded in a fine fibrillar network. Increased cadmium concentrations (10−3–10−2 M) decreased or completely paralyzed cytoplasmic streaming. No typical cytoplasmic zonation existed, while cell organelles (plastids, lipid droplets) were relocated toward the tip. The vesicular apical zone was drastically reduced, with vesicles dispersed into the subapical region. Mitochondria were distributed throughout the subapical region and among the vesicles of the tube apex. Visible ultrastructural changes in cell organelles were not observed.
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References
BJ Alloway E Steinnes (1999) Anthropogenic additions of cadmium to soils MJ McLaughlin BR Singh (Eds) Cadmium in soils and plants Kluwer Academic Dordrecht 97–123
R Athar A Masood (2002) ArticleTitleHeavy metal toxicity; effect on plant growth and metal uptake by wheat, and on free living Azotobacter Water Air Soil Pollut 138 165–180 Occurrence Handle10.1023/A:1015594815016 Occurrence Handle1:CAS:528:DC%2BD38Xltlemu7w%3D
L Borboa C De la Torre (1996) ArticleTitleThe genotoxicity of Zn(II) and Cd(II) in Allium cepa root meristematic cells New Phytol 134 481–486 Occurrence Handle10.1111/j.1469-8137.1996.tb04365.x Occurrence Handle1:CAS:528:DyaK2sXjtFWktg%3D%3D
C Chukwuma SuffixSr (1993) ArticleTitleComparison of the accumulation of cadmium, lead and zinc in cultivated and wild plant species in the derelict Enyigba-Abakaliki lead and zinc mine, Nigeria Toxicol Environ Chem 38 167–173 Occurrence Handle1:CAS:528:DyaK3sXms12qsbw%3D Occurrence Handle10.1080/02772249309357888
RM Cox (1988) ArticleTitleThe sensitivity of pollen from various coniferous and broad-leaved trees to combinations of acidity and trace metals New Phytol 109 193–201 Occurrence Handle10.1111/j.1469-8137.1988.tb03708.x Occurrence Handle1:CAS:528:DyaL1cXks1yntr0%3D
A Fusconi C Gallo W Camusso (2007) ArticleTitleEffects of cadmium on root apical meristems of Pisum sativum L.: cell viability, cell proliferation and microtubule pattern as suitable markers for assessment of stress pollution Mutat Res 632 9–19 Occurrence Handle17556012 Occurrence Handle1:CAS:528:DC%2BD2sXnslWksb8%3D
AG Gentile SM Richman AT Eaton (1973) ArticleTitleCorn pollen germination and tube elongation inhibited or reduced by commercial and experimental formulations of pesticides and adjuvants Environ Entomol 7 689–691
N Gur A Topdemir (2005) ArticleTitleEffects of heavy metals (Cd++, Cu++, Pb++, Hg++) on pollen germination and tube growth of quince (Cydonia oblonga M.) and plum (Prunus domestica L.) Fresenius Environ Bull 14 36–39
W Herth WW Franke H Bittiger A Kuppel G Keilich (1974) ArticleTitleAlkali-resistant fibrils of β-1,3- and β-1,4-glucans: structural polysaccharides in the pollen tube wall of Lilium longiflorum Cytobiologie 9 344–367 Occurrence Handle1:CAS:528:DyaE2MXhsFahsbw%3D
J Heslop-Harrison (1987) ArticleTitlePollen germination and pollen tube growth Int Rev Cytol 107 1–78 Occurrence Handle10.1016/S0074-7696(08)61072-4
H-G Heumann (1987) ArticleTitleEffects of heavy metal on growth and ultrastructure of Chara vulgaris Protoplasma 136 37–48 Occurrence Handle10.1007/BF01276316 Occurrence Handle1:CAS:528:DyaL2sXhslSntL8%3D
U Kristen R Kappler (1995) The pollen tube growth test S O’Hare CK Atterwill (Eds) Methods in molecular biology NumberInSeries43 Humana Press Totowa, NJ 189–198
U Kristen U Hoppe W Pape (1993) ArticleTitleThe pollen tube growth test: a new alternative to the Draize eye irritation assay J Soc Cosmet Chem 44 153–162 Occurrence Handle1:CAS:528:DyaK3sXlvVyht70%3D
N Masaru F Katsuhisa T Sankichi W Yutata (1980) ArticleTitleEffects of inorganic components in acid rain on tube elongation of Camelia pollen Environ Pollut 21 51–57 Occurrence Handle10.1016/0143-1471(80)90032-X Occurrence Handle1:CAS:528:DyaL3cXhvFaiurg%3D
SP McGrath (1999) Adverse effects of cadmium on soil microflora and fauna MJ McLaughlin BR Singh (Eds) Cadmium in soils and plants Kluwer Academic Dordrecht 199–218
J McKinney (1993) ArticleTitleMetals bioavailability and disposition kinetics research needs workshop July 18–19 Toxicol Environ Chem 38 1–71
MJ McLaughlin BR Singh (1999) Cadmium in soils and plants. A global perspective MJ McLaughlin BR Singh (Eds) Cadmium in soils and plants Kluwer Academic Dordrecht 1–10
Ö Munzuroglu H Gekil (2002) ArticleTitleEffects of metals on seed germination, root elongation and coleoptile and hypocotyl growth in Triticum aestivum and Cucumis sativus Arch Environ Contam Toxicol 43 203–213 Occurrence Handle12115046 Occurrence Handle10.1007/s00244-002-1116-4 Occurrence Handle1:CAS:528:DC%2BD38Xmt1Wisbo%3D
TV Noodelkoska PM Doran (2000) ArticleTitleInteractive effects of temperature and metal stress on the growth and some biochemical compounds in wheat seedlings Environ Pollut 107 315–320 Occurrence Handle10.1016/S0269-7491(99)00177-3
G Obermeyer MR Blatt (1995) ArticleTitleElectrical properties of intact pollen grains of Lilium longiflorum. Characteristics of the non-germination grain J Exp Bot 46 803–813 Occurrence Handle10.1093/jxb/46.7.803 Occurrence Handle1:CAS:528:DyaK2MXntFWks7s%3D
OECD (1994) Cadmium. Risk reduction monograph, nr 5. Environmental Directorate, OECD, Paris
LP Pellegrini M Pellegrini S Delivopoulos G Berail (1991) ArticleTitleThe effects of cadmium on the fine structure of the brown alga Cystoseira barbata forma repens Zinova et Kalugina Br Phycol J 26 1–8 Occurrence Handle10.1080/00071619100650011
PL Pfahler (1981) ArticleTitleIn vitro germination characteristics of maize pollen to detect biological activity of environmental pollutants Environ Health Perspect 37 125–132 Occurrence Handle7460877 Occurrence Handle10.2307/3429260 Occurrence Handle1:STN:280:DyaL3M7hsVSrug%3D%3D
MNV Prasad (1995) ArticleTitleCadmium toxicity and tolerance in vascular plants Environ Exp Bot 35 525–545 Occurrence Handle10.1016/0098-8472(95)00024-0 Occurrence Handle1:CAS:528:DyaK28Xht1agsbc%3D
KS Rao U Kristen (1990) ArticleTitleThe influence of the detergent Triton X-100 on the growth and ultrastructure of tobacco pollen tubes Can J Bot 68 1131–1138
H-D Reiss W Herth (1979) ArticleTitleCalcium ionophore A-23187 affects localized wall secretion in the tip region of pollen tubes of Lilium longiflorum Planta 145 225–232 Occurrence Handle10.1007/BF00454445 Occurrence Handle1:CAS:528:DyaE1MXitVSltrY%3D
G Röderer H-D Reiss (1988) ArticleTitleDifferent effects of inorganic and triethyl lead on growth and ultrasructure of lily pollen tubes Protoplasma 144 101–109 Occurrence Handle10.1007/BF01637242
T Sawidis (1997) ArticleTitleAccumulation and effects of heavy metals in lilium pollen Acta Hortic 437 153–158 Occurrence Handle1:CAS:528:DyaK2sXktFams7Y%3D
T Sawidis H-R Reiss (1995) ArticleTitleEffects of heavy metals on pollen tube growth and ultrastructure Protoplasma 185 113–122 Occurrence Handle10.1007/BF01272851 Occurrence Handle1:CAS:528:DyaK2MXnvFSgsrs%3D
KB Searcy DL Mulcahy (1985) ArticleTitleThe parallel expression of metal tolerance in pollen and sporophytes of Silene dioica (L.) Clairv., S. alba (Mill.) and Mimulus guttatus DC Theor Appl Genet 69 597–602 Occurrence Handle10.1007/BF00251110
ARD Stebbing (1998) ArticleTitleA theory for growth hormesis Mutat Res 403 249–258 Occurrence Handle9726025 Occurrence Handle1:CAS:528:DyaK1cXkslymurw%3D
MW Steer JM Steer (1989) ArticleTitlePollen tube tip growth New Phytol 111 323–358 Occurrence Handle10.1111/j.1469-8137.1989.tb00697.x
SJ Traina (1999) The environmental chemistry of cadmium. An overview MJ McLaughlin BR Singh (Eds) Cadmium in soils and plants Kluwer Academic Dordrecht 11–37
AP Turner NM Dickinson NW Lepp (1991) ArticleTitleIndices of metal tolerance in trees Water Air Soil Pollut 57–58 617–625 Occurrence Handle10.1007/BF00282925
S Ünyayar A Çelik FO Çekiç A Gözel (2006) ArticleTitleCadmium induced genotoxicity, cytotoxicity and lipid peroxidation in Allium sativum and Vicia faba Mutagenesis 21 77–81 Occurrence Handle16434449 Occurrence Handle10.1093/mutage/gel001
M Wierzbicka (1999) ArticleTitleThe effect of lead on the cell cycle in the root meristem of Allium cepa L Protoplasma 207 186–194 Occurrence Handle10.1007/BF01282999 Occurrence Handle1:CAS:528:DyaK1MXmtVSnsb8%3D
M Wojcik A Tukendorf (1999) ArticleTitleCd-tolerance of maize, rye and wheat seedlings Acta Physiol Plant 21 99–107 Occurrence Handle10.1007/s11738-999-0063-3 Occurrence Handle1:CAS:528:DyaK1MXlsFKhurw%3D
ZT Xiong YH Peng (2001) ArticleTitleResponse of pollen germination and tube growth to cadmium with special reference to low concentration exposure Ecotoxicol Environ Saf 48 51–55 Occurrence Handle11161677 Occurrence Handle10.1006/eesa.2000.2002 Occurrence Handle1:CAS:528:DC%2BD3MXmvFGmtw%3D%3D
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Dedicated to the memory of Dr. Hans-Dieter Reiss, colleague and friend
Correspondence: T. Sawidis, Department of Botany, University of Thessaloniki, 54006 Thessaloniki, Greece.
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Sawidis, T. Effect of cadmium on pollen germination and tube growth in Lilium longiflorum and Nicotiana tabacum . Protoplasma 233, 95–106 (2008). https://doi.org/10.1007/s00709-008-0306-y
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DOI: https://doi.org/10.1007/s00709-008-0306-y