Summary.
A fusion protein (F-SU/GFP) which is comprised of the surface (SU) subunit of the Friend MuLV envelope glycoprotein and the green fluorescence protein (GFP) was generated by a baculovirus expression system. The F-SU/GFP specifically bound to mammalian tissue cultured cells expressing the mCAT-1, the receptor for ecotropic murine leukemia virus (Eco-MuLV). The expression level of mCAT-1 on hematopoietic cells was measured based on the capacity of cells to absorb the F-SU/GFP. In BALB/c mice susceptible to Eco-MuLV infection, all hematopoietic cell subpopulations absorbed the F-SU/GFP with the most prominent absorption observed in the TER119+ erythroblasts. Hematopoietic cells from C4W and AKR mice did not absorb the F-SU/GFP as readily as the BALB/c cells, probably due to expression of the envelope glycoproteins of endogenous Eco-MuLVs in these mice. Although AKR mice are susceptible to Eco-MuLV infection while C4W mice are resistant, these mice showed no apparent difference in the F-SU/GFP-absorbing capacity.
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Received June 9, 1999 Accepted September 8, 2000
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Suzuki, T., Aizawa, S. & Ikeda, H. Expression of receptor for ecotropic murine leukemia virus on hematopoietic cells. Arch. Virol. 146, 507–519 (2001). https://doi.org/10.1007/s007050170159
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DOI: https://doi.org/10.1007/s007050170159