Summary.
Sequence variability in the PCR amplified cDNAs from two citrus apscaviroid isolates CVd-Ia and CVd-IIId from Spain, was analysed. CVd-IIId sequence was shown to be identical to previously described CVd-III sequences and no important variability was encountered within the viroid population. Conversely, CVd-Ia displayed population heterogeneity as shown by SSCP analysis, Sal I restriction site polymorphism and sequences of 27 CVd-Ia cloned DNAs. The CVd-Ia genomic heterogeneity is characterised by two major subpopulations with the most divergent sequences, and by the presence of individual variants, making a sequence continuum between the two major groups. Most sequence variations are clustered in the left part of the viroid molecule.
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Received October 1, 1999/Accepted April 3, 2000
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Foissac, X., Duran-Vila, N. Characterisation of two citrus apscaviroids isolated in Spain. Arch. Virol. 145, 1975–1983 (2000). https://doi.org/10.1007/s007050070070
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DOI: https://doi.org/10.1007/s007050070070