Summary.
The detection of Beet necrotic yellow vein virus (BNYVV) instored sugar beets by means of monoclonal antibodies or antibody singlechain fragments (scFv) often poses problems, because the immunodominantC-terminal epitope of the viral coat protein is readily lost due toproteolysis. Clones which produce scFv specific for protease-stableBNYVV epitopes were selected from two naive phage display libraries.Fusion proteins of the scFv with a human IgG kappa chain (expressed fromthe newly designed vector pCL) or with alkaline phosphatase,respectively, allow the ELISA detection of BNYVV even in stored sugarbeets with a sensitivity which was comparable or often higher than thatachieved with polyclonal antibodies.
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Received July 26, 1999/Accepted August 13, 1999
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Uhde, K., Kerschbaumer, R., Koenig , R. et al. Improved detection of Beet necrotic yellow vein virus in aDAS ELISA by means of antibody single chain fragments (scFv) which wereselected to protease-stable epitopes from phage display libraries. Arch. Virol. 145, 179–185 (2000). https://doi.org/10.1007/s007050050015
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DOI: https://doi.org/10.1007/s007050050015