Abstract
The complete nucleotide sequence of peach virus D (PeVD) from Prunus persica was determined. The PeVD genome consists of 6,612 nucleotides excluding the 3’ poly(A) tail and contains a single open reading frame coding for a polyprotein of 227 kDa. Sequence comparisons and phylogenetic analysis revealed that PeVD is most closely related to viruses in the genus Marafivirus, family Tymoviridae. The complete nucleotide and CP amino acid sequences of PeVD were most similar (51.1–57.8% and 32.2–48.0%, respectively) to members of the genus Marafivirus, suggesting that PeVD is a new member of this genus.
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The family Tymoviridae comprises three genera (Tymovirus, Marafivirus and Maculavirus) of plant-infecting single-stranded positive-sense viruses that are grouped based on the similarity of their replication-associated polyproteins (RPs), which contain conserved domains for methyltransferase (MTR), papain-like protease (PRO), helicase (HEL) and RNA-dependent RNA polymerase (RdRp) [11]. The marafivirus genome is distinguished by the presence of a single long open reading frame (ORF) encoding an RP along with two coat proteins (CPs) and a conserved 16-nucelotide marafibox, the latter being analogous to the tymobox of tymoviruses, from which it differs by only two or three residues [2, 3, 5, 7, 11].
Peach (Prunus persica) is an economically important and widely grown deciduous tree in the family Rosaceae. Peach trees can be infected by a number of viruses and viroids, the most notable being apple chlorotic leaf spot virus (ACLSV), prunus necrotic ringspot virus (PNRSV), apricot pseudo chlorotic leaf spot virus (APCLSV), plum pox virus (PPV), plum bark necrosis stem pitting-associated virus (PBNSPaV), prune dwarf virus (PDV), tomato ringspot virus (ToRSV), peach latent mosaic viroid (PLMVd), and hop stunt viroid (HSVd). These viruses and viroids are members of the following genera and families: ACLSV and APCLSV, genus Trichovirus of family Betaflexiviridae; PNRSV and PDV, Ilarvirus (Bromoviridae); PPV, Potyvirus (Potyviridae); PBNSPaV, Ampelovirus (Closteroviridae); ToRSV, Nepovirus (Secoviridae); PLMVd, Pelamoviroid (Avsunviroidae); and HSVd, Hostuviroid (Pospiviroidae) [10, 13]. Nectarine virus M (NeVM), which belongs to the genus Marafivirus (family Tymoviridae), has recently been reported to infect nectarine (P. persica var. nectarina) [13]. Here, we report the complete genome sequence of peach virus D (PeVD), a new marafivirus infecting peach in South Korea.
Leaves exhibiting symptoms of yellowing and slight mottling were collected from a yard-grown peach tree in Gyeongsangbuk-do, South Korea, in May 2015. Total RNA was extracted from the symptomatic leaves using a WizPrep Plant RNA Mini Kit (Wizbiosolutions, Seongnam, South Korea). Plant ribosomal RNA (rRNA) was eliminated from the total RNA using a Ribo-Zero Plant Leaf rRNA Removal Kit (Epicentre, Madison, WI, USA). A library was constructed from an rRNA-depleted RNA sample using a TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) and then sequenced at the Theragen Bio Institute (Suwon, South Korea) on an Illumina HiSeq 2500 instrument. Raw sequence reads were quality-filtered and assembled de novo using the software package Trinity v2.1.1. Assembled contigs were screened using BLAST to identify sequence similarities to other viral reference genomes in the GenBank database. Among the assembled contigs, two long contigs of 2,466 and 4,056 bp were related to marafiviruses and were designated as PeVD contigs (Fig. 1B). The 2,466- and 4056-bp contigs were assembled from 4700 reads (depth of coverage across the contig: maximum, 363; minimum, 1; mean, 189) and 3276 reads (maximum, 529; minimum, 1; mean, 79), respectively (Fig. 1A). BLASTx analysis showed that the PeVD contigs had the highest amino acid sequence similarity (56–61% identity) to NeVM (genus Marafivirus; GenBank accession no. KT273413). To confirm the PeVD contigs obtained by high-throughput paired-end sequencing, specific primer sets were designed based on the contig sequences (Supplementary Table 1). Total RNA was isolated from leaves exhibiting virus-like symptoms using a WizPrep Plant RNA Mini Kit. cDNA was synthesized from total RNA using RevertAid reverse transcriptase (Thermo Scientific, USA) with the 25-mer degenerate primer N25 and then amplified by PCR using AccuPower ProFi Taq PCR PreMix (Bioneer, Daejeon, South Korea) and the specific primer sets [4, 6]. All designed primer sets successfully produced overlapping PCR fragments of the expected size from the peach sample (Fig. 1C). The PCR products were purified and cloned into an RBC TA cloning vector (RBC Bioscience, Taipei, Taiwan), and the resulting plasmids were sequenced by GenoTech (Daejeon, South Korea). The generated sequences were consistent with those of the original contigs. The peach sample was also confirmed to be infected by PeVD. To complete the genomic sequence of PeVD, 5′- and 3′-terminal sequences were obtained using a 5′- and 3′-Rapid Amplification of cDNA Ends kit (RACE version 2.0; Invitrogen, Carlsbad, CA, USA) [9]. All overlapping fragments were assembled using the program DNAMAN v5.2.10 (Lynnon Biosoft, Quebec, Canada) [9]. The full sequence of PeVD was submitted to GenBank under accession number KY084481. Phylogenetic relationships of PeVD and other members of the family Tymoviridae were inferred from their predicted RP and CP amino acid sequences using the neighbor-joining method with 1,000 bootstrap replicates in MEGA v6.06 [12]. NC_003604 (grapevine virus A [GVA], genus Vitivirus, family Betaflexiviridae) was used as an outgroup sequence.
The complete genome of PeVD, excluding the 3′ poly(A) tail, was found to comprise 6,612 nucleotides (nt) including a 295-nt 5′ untranslated region (UTR) and a 3′ UTR of 117 nt. Similar to other marafiviruses, the PeVD genome (GenBank accession no. KY084481) was found to contain a large ORF beginning with an AUG codon at nt positions 296–298 and ending with a UAA termination codon at positions 6,494–6,496. This ORF encodes a large precursor polyprotein of 2,066 amino acids (aa) with a predicted molecular mass of 226.5 kDa (p227) possessing a number of conserved domains such as viral methyltransferase (Pfam 01660), tymovirus endopeptidase (Pfam 05381), viral (superfamily 1) RNA helicase (Pfam 01443), RdRp (pfam 00978) and tymovirus CP (Pfam 00983) as well as putative CPs with deduced molecular masses of 23.7 and 21.2 kDa (Fig. 1D). Pairwise alignment of the complete nucleotide sequence of PeVD with other members of the family Tymoviridae revealed that PeVD has more nucleotide sequence similarity to marafiviruses (51.1–57.8% identity) than to tymoviruses (48.5–50.8%) or maculaviruses (44.4–47.6%) (Supplementary Table 2). In addition, the highest aa sequence similarity of the RP of PeVD was with marafiviruses (45.6–53.5%), followed by tymoviruses (40.8–43.9%) and maculaviruses (37.8–40.1%). When compared separately, the individual conserved domain regions MTR (aa 37-318), PRO (aa 768-864), Hel (aa 961-1192) and RdRp (aa 1504-1740) of PeVD were always more similar to those of marafiviruses than to those of other tymoviruses and maculaviruses (Supplementary Table 2) [1]. MTR and RdRp of PeVD were most similar (68.1% and 78.1% identity, respectively) to the corresponding domains of oat blue dwarf virus (OBDV; genus Marafivirus). PeVD PRO was most closely related (54.6%) to that of olive latent virus 3 (OLV-3; Marafivirus), while PeVD HEL was most similar (69.0%) to NeVM (Marafivirus). The aa sequence identities between the putative CP of PeVD and comparable proteins of other members of the family Tymoviridae ranged from 21.2% with turnip yellow mosaic virus (TYMV; Tymovirus) to 48.0% with NeVM [3]. A putative marafibox of PeVD was identified at nt positions 5,768–5,783, with a putative subgenomic RNA transcription site start site of CAA located at positions 5,792–5,794 [2, 3, 5, 7]. Multiple sequence alignment of the PeVD 16-nt marafibox and initiation site with corresponding regions of NeVM and TYMV revealed that the PeVD marafibox differs by six and nine nt from marafibox and tymobox sequences, respectively (Fig. 1E). It is a divergent marafibox, probably because the complete genome nt and CP aa sequences of PeVD show only 51.1–57.8% and 32.2–48.0% identity, respectively, to members of the genus Marafivirus. Phylogenetic analysis of aa sequences of RP and CP of PeVD and other members of family Tymoviridae revealed that PeVD is more closely related to marafiviruses than to members of other virus genera (Fig. 2) [1, 3]. The complete nt sequence identities between the genomes of PeVD and other members of the family Tymoviridae ranged from 44.4% (BmMLV; Maculavirus) to 57.8% (NeVM; Marafivirus), while the CP aa sequence identities varied from 21.2% (TYMV; Tymovirus) to 48.0% (NeVM) (Supplementary Table 2). According to current species demarcation criteria, members of distinct species in the genus Marafivirus should have less than 80% overall sequence identity and less than 90% capsid protein sequence identity to other members of the genus [8, 11]. Our results thus suggest that PeVD should be regarded as a member of a distinct species in the genus Marafivirus.
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This research was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ01252403),” Rural Development Administration, Republic of Korea.
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Igori, D., Lim, S., Baek, D. et al. Complete nucleotide sequence and genome organization of peach virus D, a putative new member of the genus Marafivirus . Arch Virol 162, 1769–1772 (2017). https://doi.org/10.1007/s00705-017-3255-y
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DOI: https://doi.org/10.1007/s00705-017-3255-y