Abstract.
A novel sensitive electrochemical impedance immunoassay based on metal nanoparticle labels and ITO electrodes has been developed. First, 2-aminobenzoic acid (2-ABA) was electropolymerized onto an indium tin oxide (ITO) electrode. The coupling reagents 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysulfosuccinimide were then used to activate the electroconductive polymer to form an active ester layer which could react with amino groups of antigen. Subsequently, immunoreaction was carried out between antigen and antibody labeled with gold nanoparticles, followed by the addition of the silver enhancer solution. The charge transfer processes of [Fe(CN)6]4−/[Fe(CN)6]3− on the ITO surface were affected due to the formation of silver precipitation on the gold nanoparticles, which was determined by electrochemical impedance spectroscopy. The surface was characterized by scanning electron microscopy. Finally, a multiplexed arrayed immunosensor was is described and the samples of antibody and antibody mixture were assayed specifically. The experimental conditions such as the number of electropolymerization cycles and the time of silver enhancement were examined and optimized. The detection range of antibody labeled with gold nanoparticles was between 10.0 ng mL−1 and 10.0 µg mL−1.
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References
A Malinauskas R Holze (1998) ArticleTitleAn in situ spectroelectrochemical study of redox reactions at polyaniline-modified ITO electrodes Electrochim Acta 43 2563 Occurrence Handle10.1016/S0013-4686(97)10176-1 Occurrence Handle1:CAS:528:DyaK1cXktlKgt70%3D
S H Brewer S Franzen (2004) ArticleTitleCalculation of the electronic and optical properties of indium tin oxide by density functional theory Chem Phys 300 285 Occurrence Handle10.1016/j.chemphys.2003.11.039 Occurrence Handle1:CAS:528:DC%2BD2cXisl2ltr0%3D
J Stotter Y Show S H Wang G Swain (2005) ArticleTitleComparison of the electrical, optical, and electrochemical properties of diamond and indium tin oxide thin-film electrodes Chem Mater 17 4880 Occurrence Handle10.1021/cm050762z Occurrence Handle1:CAS:528:DC%2BD2MXotFSis7Y%3D
L X Shi Y X Lu J Sun J Zhang C Q Sun J Q Liu J C Shen (2003) ArticleTitleSite-selective lateral multilayer assembly of bienzyme with polyelectrolyte on ITO electrode based on electric field-induced directly layer-by-layer deposition Biomacromolecules 4 1161 Occurrence Handle10.1021/bm030003e Occurrence Handle1:CAS:528:DC%2BD3sXmtVSitbk%3D
C Tlili K Reybier N Jaffrezic-Renault (2003) ArticleTitleFibroblast cells: a sensing bioelement for glucose detection by impedance spectroscopy Anal Chem 75 3340 Occurrence Handle10.1021/ac0340861 Occurrence Handle1:CAS:528:DC%2BD3sXkt1Cltrk%3D
L J Yang Y B Li (2005) ArticleTitleAFM and impedance spectroscopy characterization of the immobilization of antibodies on indium-tin oxide electrode through self-assembled monolayer of epoxysilane and their capture of Escherichia coli O157:H7 Biosens Bioelectron 20 1407 Occurrence Handle10.1016/j.bios.2004.06.024 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKlurzJ
T J Gardner C D Frisbie M S Wrighton (1995) ArticleTitleSystems for orthogonal self-assembly of electroactive monolayers on Au and ITO: an approach to molecular electronics J Am Chem Soc 117 6927 Occurrence Handle10.1021/ja00131a015 Occurrence Handle1:CAS:528:DyaK2MXmt12lsbs%3D
H Hillebrandt M Tanaka (2001) ArticleTitleElectrochemical characterization of self-assembled alkylsiloxane monolayers on indium-tin oxide (ITO) semiconductor electrodes J Phys Chem B 105 4270 Occurrence Handle10.1021/jp004062n Occurrence Handle1:CAS:528:DC%2BD3MXis1Sls7s%3D
S Y Oh Y J Yun D Y Kim S H Han (1999) ArticleTitleFormation of a self-assembled monolayer of diaminododecane and a heteropolyacid monolayer on the ITO surface Langmuir 15 4960 Occurrence Handle10.1021/la9900268
D H P Hedges D J Richardson D A Russell (2004) ArticleTitleElectrochemical control of protein monolayers at indium tin oxide surfaces for the reagentless optical biosensing of nitric oxide Langmuir 20 1901 Occurrence Handle10.1021/la035795c Occurrence Handle1:CAS:528:DC%2BD2cXkvVahtg%3D%3D
C Yan M Zharnikov A Golzhauser M Grunze (2000) ArticleTitlePreparation and characterization of self-assembled monolayers on indium tin oxide Langmuir 16 6208 Occurrence Handle10.1021/la000128u Occurrence Handle1:CAS:528:DC%2BD3cXktleqs7o%3D
H Cai C Shang I M Hsing (2004) ArticleTitleSequence-specific electrochemical recognition of multiple species using nanoparticle labels Anal Chim Acta 523 61 Occurrence Handle10.1016/j.aca.2004.06.072 Occurrence Handle1:CAS:528:DC%2BD2cXnsVGgsb8%3D
M Fernandes Y Vygranenko R Schwarz M Vieira C N Carvalho (2002) ArticleTitlePhotocurrent multiplication in ITO/SiOx/Si optical sensors Vacuum 65 67 Occurrence Handle10.1016/S0042-207X(01)00407-9 Occurrence Handle1:CAS:528:DC%2BD38XotFKmug%3D%3D
J D Zhang M Oyama (2005) ArticleTitleGold nanoparticle-attached ITO as a biocompatible matrix for myoglobin immobilization: direct electrochemistry and catalysis to hydrogen peroxide J Electroanal Chem 577 273 Occurrence Handle10.1016/j.jelechem.2004.12.005 Occurrence Handle1:CAS:528:DC%2BD2MXisVWqsr8%3D
T M H Lee H Cai I M Hsing (2005) ArticleTitleEffects of gold nanoparticle and electrode surface properties on electrocatalytic silver deposition for electrochemical DNA hybridization detection Analyst 130 364 Occurrence Handle10.1039/b413143f Occurrence Handle1:CAS:528:DC%2BD2MXhs1agt7o%3D
L J Yang Y B Li G F Erf (2004) ArticleTitleInterdigitated array microelectrode-based electrochemical impedance immunosensor for detection of Escherichia coli O157:H7 Anal Chem 76 1107 Occurrence Handle10.1021/ac0352575 Occurrence Handle1:CAS:528:DC%2BD2cXjtV2gsg%3D%3D
X H Sun K D Gillis (2006) ArticleTitleOn-chip amperometric measurement of quantal catecholamine release using transparent indium tin oxide electrodes Anal Chem 78 2521 Occurrence Handle10.1021/ac052037d Occurrence Handle1:CAS:528:DC%2BD28XhvVKlt7s%3D
Y H Xu Y Gao T Li Y Du J Li E K Wang (2007) ArticleTitleHighly efficient electrochemiluminescence of functionalized tris(2,2′-bipyridyl)ruthenium(II) and selective concentration enrichment of its coreactants Adv Funct Mater 17 1003 Occurrence Handle10.1002/adfm.200600537 Occurrence Handle1:CAS:528:DC%2BD2sXlsV2itb8%3D
Billah M, Hays H C W, Millner P A (2007) Development of a myoglobin impedimetric immunosensor based on mixed self-assembled monolayer onto gold. Microchim Acta DOI: 10.1007/s00604-007-0793-0
K Y Chumbimuni-Torres Z Dai N Rubinova Y Xiang E Pretsch J Wang E Bakker (2006) ArticleTitlePotentiometric biosensing of proteins with ultrasensitive ion-selective microelectrodes and nanoparticle labels J Am Chem Soc 128 13676 Occurrence Handle10.1021/ja065899k Occurrence Handle1:CAS:528:DC%2BD28XhtVWhsL%2FP
M S Wilson W Y Nie (2006) ArticleTitleElectrochemical multianalyte immunoassays using an array-based sensor Anal Chem 78 2507 Occurrence Handle10.1021/ac0518452 Occurrence Handle1:CAS:528:DC%2BD28XisFWjtb0%3D
C Z Li Y L Liu J H T Luong (2005) ArticleTitleImpedance sensing of DNA binding drugs using gold substrates modified with gold nanoparticles Anal Chem 77 478 Occurrence Handle10.1021/ac048672l Occurrence Handle1:CAS:528:DC%2BD2cXhtVCmsLnN
L J Yang C M Ruan Y B Li (2003) ArticleTitleDetection of viable Salmonella typhimurium by impedance measurement of electrode capacitance and medium resistance Biosens Bioelectron 19 495 Occurrence Handle10.1016/S0956-5663(03)00229-X Occurrence Handle1:CAS:528:DC%2BD3sXptVGlsb0%3D
G Lillie P Payne P Vadgama (2001) ArticleTitleElectrochemical impedance spectroscopy as a platform for reagentless bioaffinity sensing Sensor Actuator B 78 249 Occurrence Handle10.1016/S0925-4005(01)00821-8
D P Tang R Yuan Y Q Chai J Y Dai X Zhong Y Liu (2004) ArticleTitleA novel immunosensor based on immobilization of hepatitis B surface antibody on platinum electrode modified colloidal gold and polyvinyl butyral as matrices via electrochemical impedance spectroscopy Bioelectrochemistry 65 15 Occurrence Handle10.1016/j.bioelechem.2004.05.004 Occurrence Handle1:CAS:528:DC%2BD2cXptFKrt7g%3D
C Gabrielli P Hemery P Letellier M Masure H Perrot M I Rahmi M Turmine (2002) ArticleTitleInvestigation of ionic surfactant-selective electrodes by EIS Electrochim Acta 47 2117 Occurrence Handle10.1016/S0013-4686(02)00083-X Occurrence Handle1:CAS:528:DC%2BD38XjvFCltbk%3D
O Devos C Gabrielli B Tribollet (2006) ArticleTitleSimultaneous EIS and in situ microscope observation on a partially blocked electrode application to scale electrodeposition Electrochim Acta 51 1413 Occurrence Handle10.1016/j.electacta.2005.02.117 Occurrence Handle1:CAS:528:DC%2BD28Xls1altg%3D%3D
J R Macdonald (1997) ArticleTitleAccurate fitting of immittance spectroscopy frequency-response data using the stretched exponential model J non-cryst solids 212 95 Occurrence Handle10.1016/S0022-3093(96)00657-6 Occurrence Handle1:CAS:528:DyaK2sXjt1SmsbY%3D
J R Macdonald (2005) ArticleTitleImpedance spectroscopy: models, data fitting, and analysis Solid state ionics 176 1961 Occurrence Handle10.1016/j.ssi.2004.05.035 Occurrence Handle1:CAS:528:DC%2BD2MXot1Cisbs%3D
J R Macdonald E Tuncer (2007) ArticleTitleDeconvolution of immittance data: some old and new methods J Electroanal Chem 602 255 Occurrence Handle10.1016/j.jelechem.2007.01.006 Occurrence Handle1:CAS:528:DC%2BD2sXjtl2hu7Y%3D
C Deslouis C Gabrielli M Keddam A Khalil R Rosset B Tribollet M Zidoune (1997) ArticleTitleImpedance techniques at partially blocked electrodes by scale deposition Electrochim Acta 42 1219 Occurrence Handle10.1016/S0013-4686(96)00290-3 Occurrence Handle1:CAS:528:DyaK2sXhsVGltr8%3D
D K Xu D W Xu X B Yu Z H Liu W He Z Q Ma (2005) ArticleTitleLabel-free electrochemical detection for aptamer-based array electrodes Anal Chem 77 5107 Occurrence Handle10.1021/ac050192m Occurrence Handle1:CAS:528:DC%2BD2MXlvVKgtr8%3D
X B Yu D K Xu D W Xu R Lv Z H Liu (2006) ArticleTitleAn impedance biosensor array for label-free detection of multiple antigen-antibody reactions Front Bioscience 11 983 Occurrence Handle10.2741/1855 Occurrence Handle1:CAS:528:DC%2BD2MXhtlSrurbE
X B Yu R Lv Z Q Ma Z H Liu Y Hao Q Li D K Xu (2006) ArticleTitleAn impedance array biosensor for detection of multiple antibody-antigen interactions Analyst 131 745 Occurrence Handle10.1039/b517148b Occurrence Handle1:CAS:528:DC%2BD28XkvFOitbw%3D
J Wang D K Xu A N Kawde R Polsky (2001) ArticleTitleMetal nanoparticle-based electrochemical stripping potentiometric detection of DNA hybridization Anal Chem 73 5576 Occurrence Handle10.1021/ac0107148 Occurrence Handle1:CAS:528:DC%2BD3MXnsVGjurY%3D
M Dequaire C Degrand B Limoges (2000) ArticleTitleAn electrochemical metalloimmunoassay based on a colloidal gold label Anal Chem 72 5521 Occurrence Handle10.1021/ac000781m Occurrence Handle1:CAS:528:DC%2BD3cXnsVaksrs%3D
H Chen J H Jiang Y Huang T Deng J S Li G L Shen R Q Yu (2006) ArticleTitleAn electrochemical impedance immunosensor with signal amplification based on Au-colloid labeled antibody complex Sensor Actuator B 117 211 Occurrence Handle10.1016/j.snb.2005.11.026
I Prieto M T Martin D Mobius L Camacho (1998) ArticleTitleElectrochemical properties of Langmuir-Blodgett mixed films consisting of a water-soluble porphyrin and a phospholipid J Phys Chem B 102 2523 Occurrence Handle10.1021/jp973284r Occurrence Handle1:CAS:528:DyaK1cXhs1Grsro%3D
T M H Lee H Cai I M Hsing (2004) ArticleTitleGold nanoparticle-catalyzed silver electrodeposition on an indium tin oxide electrode and its application in DNA hybridization transduction Electroanalysis 16 1628 Occurrence Handle10.1002/elan.200403039 Occurrence Handle1:CAS:528:DC%2BD2cXovFCqu74%3D
C Thiemann C M A BRett (2001) ArticleTitleElectrosynthesis and properties of conducting polymers derived from aminobenzoic acids and from aminobenzoic acids and aniline Synthetic Met 123 1 Occurrence Handle10.1016/S0379-6779(00)00364-7 Occurrence Handle1:CAS:528:DC%2BD3MXlvFaqtbw%3D
C M A BRett C Thiemann (2002) ArticleTitleConducting polymers from aminobenzoic acids and aminobenzenesulphonic acids: influence of pH on electrochemical behaviour J Electroanal Chem 538–539 215 Occurrence Handle10.1016/S0022-0728(02)01215-9
L L Li H Cai T M H Lee J Barford I M Hsing (2004) ArticleTitleElectrochemical detection of PCR amplicons using electroconductive polymer modified electrode and multiple nanoparticle labels Electroanalysis 16 81 Occurrence Handle10.1002/elan.200302921 Occurrence Handle1:CAS:528:DC%2BD2cXht1Ciurk%3D
H Z Huang Z G Liu X R Yang (2006) ArticleTitleApplication of electrochemical impedance spectroscopy for monitoring allergen-antibody reactions using gold nanoparticle-based biomolecular immobilization method Anal Biochem 356 208 Occurrence Handle10.1016/j.ab.2006.05.021 Occurrence Handle1:CAS:528:DC%2BD28XptFSrtLY%3D
Z P Chen J H Jiang X B Zhang G L Shen R Q Yu (2006) ArticleTitleAmplified electrochemical immunoassay using HRP labeled protein as an inhibitor to silver deposition in the presence of H2O2 Chinese Chem Lett 17 489 Occurrence Handle1:CAS:528:DC%2BD28XktlCnuro%3D
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Correspondence: Danke Xu, Beijing Proteome Research Center, State Key Laboratory of Proteomics, Beijing Institute of Radiation Medicine, 33 Life Science Park Road, Changping District, Beijing 102206, P.R. China; Junjie Zhu, Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, P.R. China
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Zhang, J., Wang, J., Zhu, J. et al. An electrochemical impedimetric arrayed immunosensor based on indium tin oxide electrodes and silver-enhanced gold nanoparticles. Microchim Acta 163, 63–70 (2008). https://doi.org/10.1007/s00604-008-0944-y
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DOI: https://doi.org/10.1007/s00604-008-0944-y