Abstract.
A high-performance amperometric glucose biosensor was developed, based on immobilization of glucose oxidase (GOx) on a copper (Cu) nanoparticles/chitosan (CHIT)/carbon nanotube (CNT)-modified glassy carbon (GC) electrode. The Cu and CNT had a synergistic electrocatalytic effect toward the reduction of hydrogen peroxide in the matrix of biopolymer CHIT. The Cu/CHIT/CNT modified GC electrode could amplify the reduction current of hydrogen peroxide greatly. Besides, the Cu/CHIT/CNT modified GC electrode reduces hydrogen peroxide at a much lower applied potential and inhibit the responses of interferents. With GOx as an enzyme model, a new glucose biosensor was fabricated. The sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal. The biosensor exhibited excellent sensitivity (the detection limit is down to 0.02 mM), fast response time (less than 4 sec), wide linear range (from 0.05 to 12 mM), and perfect selectivity.
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Correspondence: Wanzhi Wei, State key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
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Wang, Y., Wei, W., Zeng, J. et al. Fabrication of a copper nanoparticle/chitosan/carbon nanotube-modified glassy carbon electrode for electrochemical sensing of hydrogen peroxide and glucose. Microchim Acta 160, 253–260 (2008). https://doi.org/10.1007/s00604-007-0844-6
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DOI: https://doi.org/10.1007/s00604-007-0844-6