Abstract.
The preparation of fluorescent pH-sensitive nanoparticles and monitoring the pH in real samples is described. A novel fluorescent core-shell nanoparticle was developed by encapsulating the rhodamine-β-isothiocyanate (RITC) in a silica shell with a reverse microemulsion technique. The RITC encapsulation reduces RITC leaching from the silica matrix when immersed in water. The hydrophilic silica shells are made by hydrolyzing and polymerizing tetraethoxysilane (TEOS). The fluorescent core-shell nanoparticles are pH-sensitive, and the pH dynamic range of the sensing nanoparticles is between 5.0–10.0. The method offers the advantages of adequate sensitivity, accuracy and rapid detection of pH. The results are in good agreement with those using the standard glass electrode method. They show excellent stability and high reproducibility when used as pH sensors.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Martin C R, Mitchell D T (1998) Anal Chem 70: 322 A; Kobayashi Y, Martin C R (1999) Anal Chem 71: 3665
Nalwa H S (1999) Handbook of nanostructured materials and nanotechnology. San Diego, Academic Press, Vol IV, p 621
Chang S Y, Liu L, Asher S A (1994) J Am Chem Soc 116: 6739; Chang S Y, Liu L, Asher S A (1994) J Am Chem Soc 116: 6745
Chan W C W, Nie S (1998) Science 281: 2016; Bruchez M, Moronne M, Gin P, Weiss S, Alirisators A P (1998) Science 281: 2013
Santra S, Wang K, Tapec R, Tan W (2001) J Biomed Opt 6: 160; Santra S, Tapec R, Theodoropoulou N, Hebard A, Tan W (2001) Langmuir 17: 2900
P Couvreur C Dubernet F Puisieux (1995) Eur J Pharm Biopharm 41 2
T F Tadros (1993) Adv Colloid Interface Sci 461 47
R P Andres J D Bielefeld J I Henderson D B Janes V R Kolagunta C P Kubiak W J Mahoney R G Osifchion (1996) Science 273 1690
C B Murray C R Kagan M G Bawendi (1993) Science 270 1335
A Taleb C Petit M P Pileni (1997) Chem Mater 9 950 Occurrence Handle10.1021/cm960513y
A Taleb C Petit M P Pileni (1998) J Phys Chem B 102 2214 Occurrence Handle10.1021/jp972807s
C A Mirkin R L Letsinger R C Mucic J J Storhoff (1996) Nature 382 607 Occurrence Handle10.1038/382607a0 Occurrence Handle8757129
W Shentom D Pum U B Sleytr S Mann (1998) Nature 389 585
M Li H Schnablegger S Mann (1999) Nature 402 393 Occurrence Handle10.1038/46509
H A Clark R Kopelman R Tjalkens M A Philbert (1999) Anal Chem 71 4837 Occurrence Handle10.1021/ac990630n Occurrence Handle10565275
M Jing W Li Z X Zhuang X Chen X R Wang (2002) Chin J Sens Actuators 5 263
J H Duan K M Wang W H Tan Xiao-He Xiao Ch M He (2003) Chem J Chin Univ 24 255
J H Han D F Cui Y T Li H Zhang Y Z Huang et al. (2000) Sens Actuators B: Chem 66 203 Occurrence Handle10.1016/S0925-4005(00)00363-4
D H Wang W P Chen M J Wang D Yu G H Wang (2000) Sens Actuators B: Chem 66 6 Occurrence Handle10.1016/S0925-4005(99)00445-1
C Ruan K G Ong C Mungle M Paulose N J Nickl C A Grimes (2003) Sens Actuators B: Chem 69 61 Occurrence Handle10.1016/S0925-4005(03)00486-6
S Shiojiri T Hirai I Komasawa (1998) Chem Commun 14 1439 Occurrence Handle10.1039/a802588f
E Stathatos P Lianos F Delmonte D Levy D Tsiourvas (1997) Langmuir 13 4295 Occurrence Handle10.1021/la9701642
Davis S S (1997) Trends Biotechnol 15: 217; Kreuter J J (1991) Control Release 16: 169
S Santra P Zhang K M Wang R Tapec W H Tan (2001) Anal Chem 73 4988 Occurrence Handle10.1021/ac010406+ Occurrence Handle11681477
S H Alabbas D C Ashworth R Narayanaswamy (1989) Anal Proc 26 373
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gao, F., Wang, L., Tang, L. et al. A Novel Nano-Sensor Based on Rhodamine-β-Isothiocyanate – Doped Silica Nanoparticle for pH Measurement. Microchim Acta 152, 131–135 (2005). https://doi.org/10.1007/s00604-005-0418-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00604-005-0418-4