Abstract
There is increasing evidence that diabetes mellitus is characterized by an enhanced lipoprotein oxidation. We have therefore investigated whether a relationship exists between LDL oxidation and microalbuminuria, which is considered an early marker of vascular involvement in type 2 diabetic patients. We selected 12 microalbuminuric and 12 normoalbuminuric type 2 diabetic patients, and 12 control subjects comparable for age, sex and blood pressure values. Oxidatively modified plasma LDL, referred as LDL–, were measured by ion-exchange HPLC. In vitro susceptibility to oxidation of LDL was evaluated by following the kinetics of conjugated diene formation in the presence of Cu++ ions (lag-phase time). Microalbuminuric diabetic patients had a less satisfactory metabolic control and showed a higher plasma triglyceride concentration than both normoalbuminuric diabetic patients (2.21±1.01 vs 1.15±0.39 mmol/l, P<0.01) and controls (1.18±0.61 mmol/l, P<0.01). The percentage of LDL– in plasma was significantly increased in microalbuminuric diabetic patients in comparison with both normoalbuminuric diabetic patients (5.24±1.67 vs 3.13±1.22%, P<0.01) and controls (2.34±1.03%, P<0.001). LDL isolated from microalbuminuric diabetic patients had a significantly shorter lag-phase time in comparison with normoalbuminuric diabetic patients (79±11 vs 97±10 min, P<0.05) and controls (120±24 min, P<0.001). In diabetic patients a significant linear correlation was observed between the percentage of LDL– and amount of fructosamine (r=0.45, P<0.05), HbA1c (r=0.41, P<0.05), and triglycerides (r=0.65, P<0.001). An inverse correlation was found between lag-phase time and fructosamine (r=–0.5, P<0.01) and triglycerides (r=–0.59, P<0.001). This study shows that microalbuminuric type 2 diabetic patients had evidence of increased LDL oxidation, which seems to be mainly due to a poor metabolic control and a more atherogenic lipid profile.
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Received: 9 March 1998 / Accepted in revised form: 24 June 1998
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Moro, E., Zambon, C., Pianetti, S. et al. Electronegative low density lipoprotein subform (LDL–) is increased in type 2 (non-insulin-dependent) microalbuminuric diabetic patients and is closely associated with LDL susceptibility to oxidation. Acta Diabetologica 35, 161–164 (1998). https://doi.org/10.1007/s005920050123
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DOI: https://doi.org/10.1007/s005920050123