Abstract.
A small protein, designated Myk15, was found to be strongly induced in wheat (Triticum aestivum) roots colonized by the arbuscular mycorrhizal fungus Glomus intraradices. This protein, which is most abundant in root fractions characterized by strong mycorrhizal colonization, has been characterized using two-dimensional polyacrylamide gel electrophoresis and microsequencing. It has an apparent molecular mass of 15 kDa and an isoelectric point of 4.5. The N-terminal sequence has high similarity to a peptide sequence deduced from an expressed sequence tag (EST) clone derived from Medicago truncatula roots colonized by G. intraradices. This EST clone is predicted to code for a protein with a similar size and isoelectric point as Myk15. The N-terminus of the deduced M. truncatula protein contains a highly hydrophobic stretch of 24 amino acid residues preceding the region with high similarity to the Myk15 N-terminus. This hydrophobic stretch is predicted to form a transmembrane α-helix and may correspond to a cleavable targeting domain.
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Fester, .T., Kiess, .M. & Strack, .D. A mycorrhiza-responsive protein in wheat roots. Mycorrhiza 12, 219–222 (2002). https://doi.org/10.1007/s00572-002-0173-x
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DOI: https://doi.org/10.1007/s00572-002-0173-x