Zusammenfassung
Lyme-Borreliose ist eine der häufigsten von Zecken vermittelten Erkrankungen, die in der Slowakei auftreten. In dieser Studie wurde Borrelia burgdorferi sensu lato in Wirt-suchenden Zecken, die aus drei Regionen der Slowakei gesammelt worden sind, nachgewiesen und kultiviert. Zur Identifizierung der Genospezies wurden zwei Methoden eingesetzt, die RFLP (restriction fragment length polymorphism) und der "reverse line blot". Ebenso wurde die Prävalenz von B. burgdorferi s.l. in den Zecken ermittelt, welche rund 32% betrug. Von den vier identifizierten Genospezies, B. afzelii, B. burgdorferi sensu stricto, B. garinii und B. valaisiana, wurde B. garinii am häufigsten nachgewiesen.
Summary
Lyme borreliosis is one of the most common tick-borne diseases that occur in Slovakia. In this study, Borrelia burgdorferi sensu lato was detected and cultivated from questing ticks collected in three areas of Slovakia. Two methods, restriction fragment length polymorphism and reverse line blot, were used for identification of isolates and determination of the prevalence of B. burgdorferi s.l. in the ticks. The prevalence of B. burgdorferi s.l. in I. ricinus detected by reverse line blot was 31.9%. Four genospecies, namely B. garinii, B. valaisiana, B. afzelii and B. burgdorferi sensu stricto were found. B. garinii was the most prevalent genospecies.
Article PDF
Avoid common mistakes on your manuscript.
References
Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davis JP (1982) Lyme disease – a tick-borne spirochetosis? Science 216: 1317–1319
Rauter C, Hartung T (2005) Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis. Appl Environ Microbiol 71: 7203–7216
Richter D, Postic D, Sertour N, Livey I, Matuschka FR, Baranton G (2006) Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov. Int J Syst Evol Microbiol 56: 873–881
Dressler F, Ackermann R, Steere AC (1994) Antibody responses to the three genomic groups of Borrelia burgdorferi in European Lyme borreliosis. J Infect Dis 169: 313–318
Collares-Pereira M, Couceiro S, Franca I, Kurtenbach K, Schafer SM, Vitorino L, et al (2004) First isolation of Borrelia lusitaniae from a human patient. J Clin Microbiol 42: 1316–1318
De Michelis S, Sewell HS, Collares-Pereira M, Santos-Reis M, Schouls LM, Benes V, et al (2000) Genetic diversity of Borrelia burgdorferi sensu lato in ticks from mainland Portugal. J Clin Microbiol 38: 2128–2133
Sarih M, Jouda F, Gern L, Postic D (2003) First isolation of Borrelia burgdorferi sensu lato from Ixodes ricinus ticks in Morocco. Vector Borne Zoonotic Dis 3: 133–141
Gern L, Hu CM, Kocianová E, Výrosteková V, Řehaček J (1999) Genetic diversity of Borrelia burgdorferi sensu lato isolates obtained from Ixodes ricinus ticks collected in Slovakia. Eur J Epidemiol 15: 665–669
Kurtenbach K, De Michelis S, Sewell HS, Etti S, Schafer SM, Hails R, et al (2001) Distinct combinations of Borrelia burgdorferi sensu lato genospecies found in individual questing ticks from Europe. Appl Environ Microbiol 67: 4926–4929
Hanincová K, Taragelová V, Kočí J, Schafer SM, Hails R, Ullmann AJ, et al (2003) Association of Borrelia garinii and B. valaisiana with songbirds in Slovakia. Appl Environ Microbiol 69: 2825–2830
Postic D, Assous MV, Grimont PA, Baranton G (1994) Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol 44: 743–752
Alekseev AN, Dubinina HL, Van De Pol I, Schouls LM (2001) Identification of Ehrlichia spp. and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia. J Clin Microbiol 39: 2237–2242
Poupon M-A, Lommano E, Humair PF, Douet V, Rais O, Schaad M, et al (2006) Prevalence of Borrelia burgdorferi sensu lato in ticks collected from migratory birds in Switzerland. Appl Environ Microbiol 72: 976–979
Michalko J, Berta J, Magic D (1986) Geobotanic map of Czechoslovak Republic. Veda, Bratislava, pp 162
Sinsky RJ, Piesman J (1989) Ear punch biopsy method for detection and isolation of Borrelia burgdorferi from rodents. J Clin Microbiol 27: 1723–1727
Rijpkema S, Golubic D, Molkenboer M, Verbeek De Kruif N, Schellekens J (1996) Identification of four genomic groups of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in a Lyme borreliosis endemic region of northern Croatia. Expr Appl Acarol 20: 23–30
Junttila J, Peltomaa M, Soini H, Marjamaki M, Viljanen MK (1999) Prevalence of Borrelia burgdorferi in Ixodes ricinus ticks in urban recreational areas of Helsinki. J Clin Microbiol 37: 1361–1365
Makinen J, Vuorinen I, Oksi J, Peltomaa M, He Q, Marjamaki M, et al (2003) Prevalence of granulocytic Ehrlichia and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from Southwestern Finland and from Vormsi Island in Estonia. APMIS 111: 355–362
Jenkins A, Kristiansen BE, Allum AG, Aakre RK, Strand L, Kleveland EJ, et al (2001) Borrelia burgdorferi sensu lato and Ehrlichia spp. in Ixodes ticks from southern Norway. J Clin Microbiol 39: 3666–3671
Quessada T, Martial-Convert F, Arnaud S, Leudet De La Vallee H, Gilot B, et al (2003) Prevalence of Borrelia burgdorferi species and identification of Borrelia valaisiana in questing Ixodes ricinus in the Lyon region of France as determined by polymerase chain reaction-restriction fragment length polymorphism. Eur J Clin Microbiol Infect Dis 22: 165–173
Derdakova M, Beati L, Petko B, Stanko M, Fish D (2003) Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in Ixodes ricinus ticks from the Czech Republic. Appl Environ Microbiol 69: 509–516
Misonne MC, Van Impe G, Hoet PP (1998) Genetic heterogeneity of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in Belgium. J Clin Microbiol 36: 3352–3354
Jouda F, Crippa M, Perret J-L, Gern L (2003) Distribution and prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks of canton Ticino (Switzerland). Eur J Epidemiol 18: 907–912
Kirstein F, Rijpkema S, Molkenboer M, Gray JS (1997) The distribution and prevalence of B. burgdorferi genomospecies in Ixodes ricinus ticks in Ireland. Eur J Epidemiol 13: 67–72
Kurtenbach K, Peacey M, Rijpkema SG, Hoodless AN, Nuttall PA, Randolph SE (1998) Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England. Appl Environ Microbiol 64: 1169–1174
Rar VA, Fomenko NV, Dobrotvorsky AK, Livanova NN, Rudakova SA, Fedorov EG, et al (2005) Tickborne pathogen detection, Western Siberia, Russia. Emerg Infect Dis 11: 1708–1715
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Smetanová, K., Burri, C., Pérez, D. et al. Detection and identification of Borrelia burgdorferi sensu lato genospecies in ticks from three different regions in Slovakia. Wien Klin Wochenschr 119, 534–537 (2007). https://doi.org/10.1007/s00508-007-0851-y
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s00508-007-0851-y