Abstract
In the analysis of 5’ sequence elements necessary for promoter activity in pollen various promoter- reporter gene constructs usually show similar patterns of expression in pollen when assayed by either transient transformations or after stable transformations in transgenic plants. Out of several fragment lengths of the Zm13 pollen-specific promoter linked to GUS, we found that the largest fragment (–1001 to +61) showed approximately eightfold higher expression in pollen of transgenic plants than transient transformations by microprojectile bombardment. No other promoter fragment tested showed this difference. Titration of a limited number of transcription factors by the large number of potential binding sites in this promoter fragment along with the large number of promoter copies introduced during transient transformation are possible explanations for this observation.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 13 October 1999 / Revision accepted: 7 December 1999
Rights and permissions
About this article
Cite this article
Hamilton, D., Schwarz, Y., Rueda, J. et al. Comparison of transient and stable expression by a pollen-specific promoter: the transformation results do not always agree. Sex Plant Reprod 12, 292–295 (2000). https://doi.org/10.1007/s004970050197
Issue Date:
DOI: https://doi.org/10.1007/s004970050197