Abstract.
Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia. In this study, Dynal IMS was evaluated on the basis of recovery efficiencies of Giardia cysts for various IMS operational conditions. The average recoveries for Giardia in deionized, treated and raw water samples were 82.6±12.2% (n=6), 75.6±15.2% (n=3), and 70.6±18.2% (n=3), respectively. Significant changes in recovery were observed by altering the debris ratio and the debris components of water samples. Changing the reaction volume within the same vessel had no significant effect on cyst recovery efficiencies. However, prolonging the reaction time did increase recovery efficiencies.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Electronic Publication
Rights and permissions
About this article
Cite this article
Hsu, BM., Huang, C., Lai, YC. et al. Evaluation of immunomagnetic separation method for detection of Giardia for different reaction times and reaction volumes. Parasitol Res 87, 472–474 (2001). https://doi.org/10.1007/s004360100377
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s004360100377