Abstract
As a first step in the biochemical and biomedical analyses of flagella from Trichomonas vaginalis the flagella were isolated, purified, and analyzed. The flagella were detached by mechanical shearing and a crude flagellar preparation was obtained by low-speed differential centrifugation. The crude flagellar preparation was subjected to further purification by discontinuous sucrose density-gradient centrifugation. Electron micrographs (EM) of the purified flagella showed the typical 9 + 2 axonemal arrangement. The structural integrity and the flagellar membrane were not destroyed by the deflagellation method or the purification scheme employed. The flagellar preparations were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified preparation contained many flagellar enriched proteins ranging from 20 to 120 kDa. Three major proteins of 65 kDa and a doublet of about 50–58 kDa were observed. The protein patterns and EM appearance of the fractions were highly reproducible.
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Received: 12 December 1997 / Accepted: 5 June 1998
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Jemilohun, P. Isolation and characterization of flagella from Trichomonas vaginalis . Parasitol Res 84, 800–805 (1998). https://doi.org/10.1007/s004360050491
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DOI: https://doi.org/10.1007/s004360050491