Abstract.
An improved method to obtain a large number of axenic larvae of Angiostrongylus vasorum from fecal samples was developed in the present study. The procedure here in reported consisted of obtaining larvae using a modified Baermann technique, followed by an additional filtration step. This isolation technique recovered almost 90% of the living larvae in a clean preparation. Isolated larvae were submitted to decontamination treatments with either sodium hypochlorite or antibiotic cocktail solutions. The axenic status, as confirmed by oral inoculation of decontaminated larvae into germ-free mice, was only achieved using larvae treated with 0.5% sodium hypochlorite solution for 10 min. The isolation and decontamination treatment did not affect larval viability. Treated larvae remained viable and infective to the invertebrate host.
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Barçante, .J., Barçante, .T., Dias, .S. et al. A method to obtain axenic Angiostrongylus vasorum first-stage larvae from dog feces. Parasitol Res 89, 89–93 (2003). https://doi.org/10.1007/s00436-002-0719-z
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DOI: https://doi.org/10.1007/s00436-002-0719-z