Abstract
The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. Single muscle fibres were dissected from m. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 μm) for 8 to 22 days. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200–600 nmol/L), (2) 0.2% bovine albumin or (3) 0.2% bovine albumin in combination with insulin (120 nmol/L). In culture medium with insulin, 50% of the muscle fibres became in-excitable within 7–12 days, whereas the other 50% were stable. Caffeine contractures of in-excitable muscle fibres produced 80.4 ± 2.4% of initial peak tetanic force, indicating impaired excitation–contraction (E–C) coupling in in-excitable fibres. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In contrast, muscle fibres cultured with both albumin and insulin showed an increase in tetanic force and fibre cross-sectional area of 19.6 ± 2.8% and 32.5 ± 4.9%, respectively, (means ± SEM.; P = 0.007) after 16.3 ± 1.7 days, whereas the number of sarcomeres in series remained unchanged. We conclude that albumin prevents muscle fibre damage and preserves E–C coupling in culture. Furthermore, albumin is important in regulating muscle fibre adaptation by a synergistic action with growth factors like insulin.
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Introduction
Chronic catabolic conditions (e.g. chronic heart failure, chronic obstructive pulmonary disease, cancer and HIV) are characterised by severe muscle wasting causing limited mobility of millions of patients [40]. Prevention of muscle atrophy and induction hypertrophy requires insight in the mechanisms regulating protein synthesis and breakdown. Many genes and physiological stimuli are involved in the regulation of adaptation of muscle size [14, 2, 19]. Of these, insulin and insulin-like growth factors (IGFs) are key regulators of muscle protein synthesis and degradation [12, 14, 15]. The mechanisms by which insulin and IGFs stimulate the synthesis and inhibit the degradation of muscle proteins are subject of controversy (cf. [44, 22, 10]). A major reason for this is that the effects of insulin and insulin-like factors depend strongly on several other factors [29]. Insulin and IGF-1 activate the phosphatidylinositol 3-Kinase (PI3K)–Akt–mammalian target of rapamycin (mTOR) pathway [29, 14]. In myoblasts, activity of this pathway is transiently increased, indicating that exposure time is a determinant of the effects of insulin and IGFs [23]. The availability of amino acids as well as the type and magnitude of exercise are important for the signalling effects of insulin on mRNA translation [29]. The ability of IGF-1 to induce proliferation of myoblast is critically dependent on unknown serum components [12].
Because unravelling how insulin and IGFs interact with other factors and regulate adaptation of muscle phenotype requires independent manipulation of the regulators and signalling pathways, we developed a serum-free culture system for longitudinal study (months) of mature, single Xenopus muscle fibres [35, 28].
For initial experiments, 50% of cultured muscle fibres (five out of ten) produced stable peak tetanic forces after electrical stimulation for at least 2 weeks [26]. Tetanic force of the other fibres was reduced by >50% during the first week. These fibres could only be activated by caffeine, indicating failure of excitation–contraction (E–C) coupling. Despite supra-physiological insulin concentrations in the culture medium, no hypertrophy or adaptation of the number of sarcomeres in series occurred [26], even when the fibres were cultured for more than 3 weeks at 5% above slack length [28, 27]. These observations indicate that insulin alone is not sufficient to induce muscle fibre hypertrophy despite availability of amino acids. Apparently, for active insulin signalling in serum-free cultured mature muscle fibres, at least one additional serum component is required. A similar conclusion was reached previously with respect to IGF-1 signalling in myoblasts [12]. A plasma protein that could be essential in insulin–IGF-1 signalling may be albumin because: (a) albumin is a carrier of hormones, growth factors and amino acids [37, 45], (b) reduced plasma albumin levels during ageing are associated with a loss of muscle mass and decrease of maximum active muscle force [3, 46], (c) culture of myotubes at high strain stimulates hypertrophy as well as the uptake of albumin from the extracellular fluid [1]. In addition, albumin may also prevent muscle fibre damage because albumin is: (d) an extracellular antioxidant [18, 37], (e) capable of preserving the calcium-pump activity of the sarcoplasmic reticulum of cardiomyocytes [5] and (f) increasing the viability of C2C12 cells [8]. Based on these results, we hypothesise that albumin preserves E–C coupling in cultured muscle fibres and that albumin is involved in the induction of muscle hypertrophy. To test these hypotheses, we investigated the effects of albumin and insulin separately, as well as in combination on mature, single muscle fibres during long-term culture.
Materials and methods
Animals and culture system
Treatment of animals was in accordance with the guidelines and regulations concerning animal welfare and experimentation set forth by Dutch law and approved by the Committee on Ethics of Animal Experimentation at the VU University Amsterdam. In brief, after cooling in ice water for about 15 min, Xenopus laevis (females, 9–10 cm body length, n = 22) were killed by decapitation and both iliofibularis muscles were excised. The muscles were allowed to recover for 1 h in sterilised (0.22-μm filter), oxygenated Ringer solution (NaCl, 116.5 mM; KCl, 2.0 mM; CaCl2, 1.9 mM; NaH2PO4, 2.0 mM; ethylene glycol tetraacetic acid, 0.1 mM; pH 7.2). After recovery, both muscles were transferred to aseptic dissection troughs [32]. Highly oxidative single muscle fibres (type 2/3 according to the classification of Lännergren and Smith [32]) were carefully dissected under aseptic conditions using fine-tipped forceps and scissors under observation through a microscope fitted with dark-field illumination (one or two fibres per animal). Small platinum hooks were tied to the trimmed tendons using 20-μm-diameter sterile polyamide thread. The platinum hooks and tools for dissection were sterilised using 70% ethanol. Before transferring the isolated fibre to the culture chamber, fibre diameters and the number of sarcomeres in series were determined (see below).
All parts of the culture chamber [35] were sterilised using 70% ethanol. The fibre was mounted at the target length between a force transducer (AE801, SensoNor, Horten, Norway) and an adjustable rod (Fig. 1).
The chamber contained 0.8 ml of culture medium. The stock solution of the culture medium consisted of 66% Dulbecco’s modified Eagle’s medium/F12 (GibcoBRL, Life Technologies, Breda, The Netherlands) with 100 U mL−1 penicillin per 100 μg mL−1 streptomycin (GibcoBRL), 5 mM sodium phosphate, 1 mM creatine (Sigma-Aldrich, Zwijndrecht, The Netherlands), 0.5 mM l-carnitine (Sigma-Aldrich, Zwijndrecht, The Netherlands). To investigate the effects of insulin, albumin and their combination, the stock medium was supplemented with one of the following agents: (1) 1 μg mL−1 bovine insulin (Serva, Heidelberg, Germany), (2) 10 μg mL−1 bovine insulin, (3) 0.2% bovine serum albumin (BSA; Sigma-Aldrich, Zwijndrecht, The Netherlands) and (4) both 1 μg mL−1 bovine insulin and 0.2% BSA.
As insulin may bind to glass and tubing, the final insulin concentration in the culture chamber was measured using an immunoradiometric assay (Biosource Europe, Nivelles, Belgium). To determine the actual insulin concentration in the culture medium during the culture, human insulin (Sigma-Aldrich) was used as a model for bovine insulin. The final insulin concentration in the culture chamber when 1 μg mL−1 bovine insulin was supplemented was 6.3 ± 1.8 nmol/L (mean ± SEM), which is 35 times lower than the value in the stock solution (170 nmol/L). Compared to the Xenopus plasma insulin concentration of 10.46 μU/mL [36], the insulin concentration in the culture chamber is about 1,000-fold higher (assuming an insulin activity of 28 U/mg). When 10 μg mL−1 bovine insulin was added to the stock culture medium, the insulin concentration in the culture chamber was between 200 and 400 nmol/L. Addition of 1 μg mL−1 insulin to a stock solution containing a 0.2% BSA yielded an insulin concentration of 121.4 ± 2.9 nmol/L in the culture chamber.
Before culture, the number of sarcomeres in series and fibre cross-sectional area (CSA) were determined as described previously [26]. In the dissection chamber, fibres were stretched to just over slack length. Sarcomere length was determined every millimeter along the length of the fibre using laser diffraction and the mean sarcomere length was calculated. Subsequently, muscle fibre length was adjusted to a length corresponding to a mean sarcomere length of 2.3 μm (l2.3 μm, i.e. approximately fibre slack length). At this fibre length, the CSA was determined by measuring the smallest and largest diameters of the fibre at three positions along the length of the muscle fibre. The CSA was calculated assuming an elliptical cross section and muscle fibre CSA was taken as the mean of these three values.
After these measurements, the fibres were mounted in the culture chamber and length was adjusted to l2.3 μm. The culture chamber was transferred to an incubator kept at 20°C and tubing for gas and culture medium supply were connected. Fresh culture medium was continuously pumped through the chamber at a rate of about 0.5 mL h−1 and was equilibrated with air, containing 2.4% CO2. Final pH was 7.6 and osmolarity was 235–250 mOsm kg−1. The oxygen tension of the culture medium sampled from the chamber was 120 to 130 mmHg.
During subsequent days, twitch and tetanic forces were measured once every 24 h. The fibre was stimulated (0.4-ms biphasic current pulses, 1.25 times threshold voltage) via platinum plate electrodes flanking the full length of the fibre to produce two twitches, one tetanus (50 Hz, 260-ms duration) and a post-tetanic twitch (300 ms after the last pulse of the tetanus). After culture, muscle fibres were again transferred to the dissection chamber filled with Ringer solution and the fibre CSA as well as the number of sarcomeres in series was determined again.
Histochemistry
After culture, fibres were embedded at l2.3 μm in gelatin Ringer (15% (w/v), pH 7.2), and frozen in liquid nitrogen. Within 3 days after freezing in liquid nitrogen, cross sections (10 and 16 μm thick) were cut using a cryostat at −20°C and mounted on glass slides coated with Vectabond (Vector Laboratories, Burlingame, USA). The 16-μm-thick sections were immediately assayed for succinate dehydrogenase (SDH, see below). The 10-μm sections were dried and subsequently stored at −80°C until further use (3–12 months).
The following stainings were performed for the following variables:
Myofibrillar adeno triphosphatase (ATPase) activity was determined as described in detail previously [51]. Sections (10 μm thick) were air-dried and then incubated for calcium-stimulated myofibrillar ATPase for 10 min at 20°C. The incubation medium consisted of 83-mM Tris–maleate buffer, 5.6 mM Pb(NO3)2, 10 mM CaCl2 and 9 mM Na2ATP, pH.7.2. After the incubation, the sections were developed in Na2S.
Glycogen contents in the muscle fibres were determined with the periodic acid–Schiff (PAS) staining reaction [55, 52]. Sections (10 μm thick) were air-dried and fixed in 4% formaldehyde in 20 ml 0.2 M imidazol and 180 ml acetone. Subsequently, sections were oxidised in 44 mM periodic acid for 30 min in a water bath at 20°C. After oxidation, sections were briefly washed in 0.1 M HCl, stained with Schiff's reagent for 25 min at 20°C.
Neutral lipid content was examined using oil red O staining [31]. Sections (10 μm thick) were air-dried and fixed in phosphate-buffered 4% formaldehyde (Merck, Amsterdam, The Netherlands) for 1 h and washed three times for 30 s in water. Sections were incubated for 30 min in a 60% (v/v) oil red O stock solution. The oil red O stock solution consisted of 500 mg oil red O (Fluka Chemie, Zwijdrecht, The Netherlands) dissolved in 100 ml 60% triethyl-phosphate ((C2H5O)3PO, Fluka Chemie). Prior to incubation, this solution was filtered through Schleicher and Schuell paper number 595 (Whatman, the Netherlands) to remove crystallised oil red O. After incubation in oil red O medium, sections were washed three times for 30 s in water and subsequently mounted in glycerin–gelatin.
SDH was determined as described by van der Laarse et al. [49]. The activity determined in this way is proportional toVO2max of muscle fibres. Muscle cross sections (16 μm thick) were air-dried for 15 min and incubated in the dark at 20°C for 45 min. The incubation medium consisted of 37.5 mM sodium phosphate buffer, 75 mM sodium succinate, 5 mM sodium azide and 0.4 mM tetranitro blue tetrazolium, pH 7.6.
Cytochrome c oxidase activity (CYTOX) was assayed as described by Old and Johnson [42]. Sections (10 μm thick) were air-dried and subsequently incubated for minutes in oxygenated media containing 5 mM diaminobenzidine and 100 μM cytochrome c.
All sections were mounted in glycerin–gelatin and stored at 4°C in the dark until staining intensity was measured.
Microdensitometry
The absorbance values of the final reaction products in the sections were determined using a Leica DMRB microscope (Wetzlar, Germany) fitted with calibrated grey filters using different interference filters. Absorbances for SDH were determined at 660 nm (A660), PAS myofibrillar ATPase at 550 nm (A550), oil red O at 480 nm (A480) and COTOX at 436 nm (A436). Images were recorded with a ×20 objective and a Sony XC-77CE camera (Towada, Japan) connected to an LG-3 frame grabber (Scion; Frederick, MD, USA) in an Apple Power Macintosh computer. Recorded images were analysed with the public domain programme NIH-Image V1.61 (US National Institutes of Health, available at http://rsb.info.nih.gov/nih-image/). Grey values were converted to absorbance values per pixel using the grey filters and a third-degree polynomial fit in the calibrate option of NIH-image. Morphometry was calibrated using a slide micrometre and the set scale option in NIH-image, taking the pixel-to-aspect ratio into account.
Statistics
Up to 8 days in culture, significance of changes in tetanic force during culture and differences between the different culture media were tested by two-way analysis of variance with repeated measures on one factor. After 8 days of culture, changes in tetanic forces were tested by regression analyses for repeated measures according to [4]. Changes in muscle fibre CSA and number of sarcomeres in series were tested by paired t-tests. A P value of less than 0.05 was considered significant. Values are presented as means ± SEM.
Results
Excitability, tetanic force and adaptation of muscle fibre size
Figure 2 shows tetanic force recordings of individual muscle fibres during culture in the different culture media. Half of the muscle fibres (6 out of 12) cultured in medium with supplements of 1 μg/ml insulin exclusively (which gives a final concentration in the culture medium of 6.3 ± 1.8 nmol/L, see methods), became in-excitable within 7–12 days. When activated by 20 mM caffeine, in-excitable muscle fibres produced 80.4 ± 2.4% of the initial tetanic force. Fibres that were not tested by caffeine developed hyper-contractions and died. The excitable muscle fibres showed a mean decline of peak tetanic force of 12.4 ± 3.4% during the first 4 days of culture (Fig. 3). Subsequently, force returned to initial values (Figs. 2a and 3). Muscle fibre CSA and the number of sarcomeres in series were unchanged (Fig. 4a and b).
Five muscle fibres cultured with BSA supplement exclusively remained electrically excitable for at least 10 days. Tetanic force remained unchanged until day 7, after which it decreased by 0.54% per day ± 0.12% (r = −0.76, P < 0.0001) of the initial value (Figs. 2c and 3). Muscle fibre CSA and number of sarcomeres in series were unchanged (Fig. 4a and b).
For five muscle fibres cultured with supplements of both 0.2% BSA and 1 μg mL−1 bovine insulin, the final insulin concentration within the culture chamber was estimated to be 121.4 ± 2.9 nmol/L (i.e. @20 times higher than in medium supplemented with insulin only). These fibres were electrically excitable for at least 10 days, confirming effects of albumin on preserving E–C coupling. Tetanic force increased by 1.3% per day ± 0.15% of the initial value (Figs. 2d and 3). CSA of the muscle fibres increased by 32.5 ± 4.9% (P = 0.007) after 16.3 ± 1.7 days (Fig. 4b). This increase correlates with the increase in tetanic force (Spearman's rank correlation coefficient = 0.98, P < 0.001; Fig. 4c). The number of sarcomeres in series was unchanged (Fig. 4a).
To test whether the BSA + insulin-induced hypertrophy could be explained by the higher insulin concentration in the culture chamber (20× higher compared to insulin-supplemented exclusively), another group of muscle fibres was cultured in the presence of 200–600 nmol/L insulin for periods varying from 8 to 24 days (n = 4). Even at these extremely high insulin concentrations, one of these muscle fibres was in-excitable or appeared damaged within 1 week and produced a force of 91% of the initial peak tetanic force after addition of 20 mM caffeine. The other appeared to be damaged after 1 week. Muscle fibres with unimpaired E–C coupling did not increase tetanic force (Figs. 2b and 3), the number of sarcomeres in series (Fig. 4a) or CSA (Fig. 4b). We conclude that insulin alone even at high concentrations does not induce hypertrophy or preserve E–C coupling.
Myofibrillar ATPase activity, cytoplasmic contents of neutral lipids, glycogen and mitochondrial enzymes
In order to investigate the cause of the difference in the relative increase of tetanic force with that of the fibre CSA (i.e. increase in CSA being twice as high as the increase in tetanic force), myofibrillar ATPase activity, glycogen and neutral lipid contents, as well as mitochondrial enzyme activities were determined in cryosections of muscle fibres which had been cultured in the presence of albumin exclusively or BSA + insulin. The results of the different assays are shown in Fig. 5. Myofibrillar ATPase activity was measured to determine whether the synthesised myosin heavy chains were functional. The other assays were performed to determine whether the changes in CSA were due to accumulation of cytoplasmic components. Figure 5a–c show that the mean myofibrillar ATPase activity was not different between muscle fibres cultured in the presence of BSA and those cultured in the presence of BSA + insulin.
PAS staining of cryosections of muscle fibres cultured in the presence of albumin exclusively or BSA + insulin show that glycogen contents in these muscle fibres were not different (Fig. 5d–f).
To investigate whether the hypertrophic muscle fibres excessively accumulated fatty acids, neutral lipid contents were investigated in freshly frozen muscles as well as in muscle fibres which had been cultured for at least 10 days in culture medium supplemented with BSA or BSA + insulin. In freshly frozen muscle cross sections, the absorbance of neutral lipid staining was related to the mitochondrial enzyme activities (data not shown). For low oxidative muscle fibres (SDH absorbance values ranging from 0.05 to 0.10), the oil red O absorbance increased with SDH activity from 0.01 to 0.05. For the high oxidative muscle fibres (SDH absorbances varying from 0.10 to 0.31), the oil red O absorbances ranged from 0.03 to 0.08. Interestingly, the muscle fibres which were cultured in the presence of BSA and BSA + insulin (mean SDH absorbance varying from 0.12 to 0.28, see Fig. 5j–l) showed considerably higher oil red O absorbance values ranging from 0.17 to 0.35, which indicates that the muscle fibres synthesised and stored fatty acids during culture (Fig. 5g–i) but that final concentrations were the same for both culture conditions.
The mean SDH and CYTOX activity did not differ between muscle fibres cultured in the presence of BSA and those cultured in the presence of BSA + insulin (Fig. 5j–o).
The histological assays show that the increase in CSA and reduction in tetanic tension of fibres cultured in the presence of BSA + insulin were not due to excessive accumulation of cytoplasmic components and/or reduced myofibrillar ATPase activities.
Discussion
Albumin preserves E–C coupling
We investigated the effects of albumin on the survival of mature muscle fibres during long-term culture and whether albumin plays a role in the induction of hypertrophy. Albumin is shown to be important for the survival of isolated muscle fibres in serum-free culture medium. The present results show that for half of the muscle fibres cultured in medium supplemented with insulin exclusively, tetanic force upon electrical activation decreased by more than 50% during 1 week of culture, whereas caffeine-induced contractions yielded normal forces (i.e. similar to the initial values). As this has been shown also for freshly dissected muscle fibres [33], these results suggest that the contractile apparatus of the in-excitable fibres was still functional and that the sarco-endoplasmic reticulum could still release Ca2+, but the ryanodine receptor could not be activated electrically. The question is raised by what mechanism albumin preserves E–C coupling. In dog cardiac myocytes and rabbit slow skeletal muscle fibres, albumin acts as an antagonist of an endogenous inhibitor of the Ca2+-pumping activity of the SR in vitro [5]. However, in vivo, albumin has been localised in the interstitial space [20, 56] as well as in the T-tubular system but could not be detected in the cytoplasm [30, 9]. Therefore, an antagonistic, cytosolic effect on the inhibition of the Ca2+-pumping activity of the SR during culture can be debated. As albumin is a sacrificial antioxidant [18, 37], its binding to the sarcolemma may scavenge oxygen radicals and thereby protect it. As yet, it remains unclear by what mechanism albumin preserves the E–C coupling and protects mature muscle fibres during culture. The substantial improvement of the survival and preservation of the E–C coupling of muscle fibres by albumin warrants further research in the protective mechanisms.
Hypertrophy by synergy of insulin and albumin
In the presence of albumin, insulin has a strong hypertrophic effect on cultured muscles fibres (increase in fibre CSA of >30% in 2 weeks), whereas insulin without albumin has not. Although the increase in muscle fibre CSA correlates with the increase in tetanic force, the increase in tetanic force was less than the increase in CSA. As a result, tetanic tensions of the fibres that hypertrophied decreased, whereas tensions of fibres that were cultured in medium supplemented with albumin exclusively increased. The discrepancy in magnitude of hypertrophy and change in peak tetanic force seems not explained by an increase in cytoplasmic components other than contractile proteins. For muscle fibres that showed an insulin-induced increase of CSA between 25% and 50%, the glycogen content, as well as SDH and CYTOX activities increased during culture but at the end of culture the concentration and activities were not different between control and insulin–BSA-supplemented cultured muscle fibres and were similar to the concentrations measured in freshly frozen muscle [50, 52]. As for intact rabbit muscles, both SDH and CYTOX activities correlate with the mitochondrial density [43] and as SDH activities in intact Xenopus muscle fibres correlate with VO2max [49], the increases in mitochondrial enzyme activities may indicate that BSA + insulin increased the biosynthesis of mitochondria. The neutral lipid concentrations in control and insulin-supplemented conditions were similar but substantially higher than those in freshly frozen muscle fibres. In addition, for cultured control and BSA + insulin-induced hypertrophic muscle fibres, the myofibrillar ATPase activity did not differ and was similar to those measured in freshly frozen sections [50]. Together, we conclude that the lower tetanic tensions of the hypertrophied muscle fibres cannot be explained by changes in the structural parametres studied.
In addition to its function in glycogen homeostasis in muscle, insulin regulates muscle protein synthesis and degradation via binding to the insulin or IGF-1 receptor [17, 53, 29]. Binding of insulin to the insulin–IGF-1 receptor activates the PI3K–Akt–mTOR pathway, which increases the rate of protein translation and inhibits the expression of E3 ubiquitin ligases [7, 34]. Furthermore, insulin and IGF-1 are also involved in the regulation of proliferation and differentiation of myoblasts and satellite cells [11, 15]. The present finding that insulin exclusively does not induce hypertrophy of mature muscle fibres but does so in the presence of albumin indicates that hypertrophy of mature muscle fibres occurred via a synergistic effect of insulin and albumin. We assume that a synergistic effect of albumin and insulin has played a role in the insulin signalling in previous serum-based culture experiments as well as during in vivo experiments. The question remains by what mechanism albumin facilitates insulin signalling.
Albumin has been shown to be a protein and drug carrier [57, 37], but this function does not seem to be critical in vitro because insulin concentrations in the culture medium were at least thousandfold higher than in Xenopus blood plasma. In addition, it can be hypothesised that either insulin signalling via the insulin–IGF-1 receptor is more effective if insulin is bound to albumin and/or that albumin facilitates the binding of insulin with the insulin or IGF-1 receptor. Regarding the first possibility, the IGF-binding proteins in the extracellular matrix affect ligand–receptor interactions and signalling activities of IGFs in different cell types [6, 47]. To the best of our knowledge, a direct role of albumin in insulin and IGF-1 signalling has not been indicated. Indirectly, pre-treatment of L6 skeletal muscle cells by glycated albumin has shown impaired insulin-induced insulin receptor substrate phosphorylation in these cells [39], indicating that modified albumin can prevent proper binding of insulin with its receptor. In addition, for rat substrates, long-chain fatty acids have been indicated to modulate their biological activity [13, 41].
Regarding the second possibility, it can be hypothesised that albumin is involved in modulating the extracellular matrix of the muscle fibre, like that of endothelial cells. Albumin is a specific modulator of the glycocalyx, in particular of heparan sulfate, by preventing its collapse and by facilitating shear-loading-induced mechanotransduction in these cells [48, 24]. As charges exposed by many proteins of the glycocalyx are mainly negative and albumin has both positive and negative charges at physiological pH [25], albumin could be involved in modulating the glycocalyx and insulin–IGF-1 signalling. During culture of muscle fibres in medium supplemented with insulin exclusively, the lack of insulin-induced hypertrophy may have been due to collapse of the proteoglycans at the basal lamina as suggested for endothelial cells, which may prevent binding of insulin to the insulin and/or IGF-1 receptors.
Whether albumin plays a role in the regulation of muscle fibre atrophy and hypertrophy in vivo remains to be determined. There are observations suggesting a role of albumin in the regulation of muscle size in vivo. Chronic low-frequency stimulation of rabbit tibialis anterior muscle causes an increase in interstitial albumin content [20]. Albumin contents in mice soleus muscle increased during different dis-use conditions [56]. Elevated albumin levels have been shown in dystrophic muscle of mdx mice [9]. In addition, lower serum albumin concentrations in elderly (>75 years) are independently associated with a loss of muscle mass and strength (i.e. after adjustment for degree of physical activity, elevated inflammatory status and low protein intake) [3, 46, 54]. These results support our finding that albumin is crucial for insulin-induced muscle fibre hypertrophy.
Adaptation of the number of sarcomeres in series
The present data indicate that insulin stimulates hypertrophy but does not increase the number of sarcomeres in series of muscle fibres in culture. Muscle fibres were cultured at a mean sarcomere length of 2.3 μm, which is slightly higher than the length at which actin and myosin show maximum overlap [16]. An important stimulus for in vivo adaptation of the number of sarcomeres in series is the length at which the muscle is operating most of the time (actively or passively) [21]. Immobilisations of rat and rabbit gastrocnemius medialis muscles at extended lengths strongly increase the expression of IGFs [58, 38]. As insulin can stimulate protein synthesis via the IGF-1 receptor, the lack of changes in the number of sarcomeres in series during culture in the presence of albumin + insulin suggests that other stimuli are required to increase the serial sarcomere number. This indicates that addition of sarcomeres is either induced by the combination of a high muscle strain and IGF-1–insulin, by paracrine signalling at the muscle fibre tendon, which may be ineffective in culture or that other biochemical and/or mechanical signals related to mechanical interactions with neighbouring muscle fibres by myofascial pathways are involved, which have been removed by the isolation of the muscle fibre [22].
In summary, we used a serum-free culture medium for long-term culture of mature single fibres to investigate effects of albumin and insulin under well-defined conditions. Supplements of albumin in serum-free culture medium are crucial to maintain fibres in good condition as it prevents muscle fibre damage and preserves the E–C coupling. Furthermore, insulin-induced hypertrophy is critically dependent on albumin. We hypothesise that albumin facilitates the binding of insulin with the insulin and/or IGF-1 receptor.
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Acknowledgments
We thank students Lluis Millan Arino and Alba Jené Sanz for their support in histochemistry. This work was partially supported by research grants from the Haak Bastiaanse Kuneman Stichting and the Van Coeverden Adriani Stichting.
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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
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Jaspers, R.T., van Beek-Harmsen, B.J., Blankenstein, M.A. et al. Hypertrophy of mature Xenopus muscle fibres in culture induced by synergy of albumin and insulin. Pflugers Arch - Eur J Physiol 457, 161–170 (2008). https://doi.org/10.1007/s00424-008-0499-0
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DOI: https://doi.org/10.1007/s00424-008-0499-0