Abstract
In addition to producing acute neuronal necrosis within selectively vulnerable brain regions, our recent studies have shown that global cerebral ischemia may also be followed by protracted degenerative changes occurring over the course of 10 weeks. Chronic brain pathology may be associated with the abnormal deposition of β-amyloid precursor protein (βAPP). In the present study, we used a monoclonal antibody to the N-terminal portion of βAPP to characterize the brains of rats surviving 1–10 weeks following 10 min of global brain ischemia produced by bilateral carotid artery occlusions plus systemic hypotension. After ischemia, increased βAPP immunolabeling emerged in several brain regions. In the hippocampus, granular deposits appeared in the damaged CA1 area by 2 weeks, and by 4–10 weeks the remnants of necrotic CA1 neurons were also immunolabeled. In striatum and thalamus, regions with necrotic cell death also revealed granular βAPP deposits. The neocortex was devoid of overt ischemic neuronal damage but revealed prominent βAPP immunoreactivity. Large ovoid deposits of low-density βAPP immunostaining occurred in cortical neurons at 1–2 weeks. At 4–10 weeks, large round or oval deposits immunoreactive for βAPP appeared in several cortical regions. The highest density of deposits was seen in the temporal and piriform cortices. Our results indicate that abnormal βAPP deposition may result from ischemic as well as chronic neurodegenerative processes.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 3 July 1998 / Revised, accepted: 6 October 1998
Rights and permissions
About this article
Cite this article
Lin, B., Schmidt-Kastner, R., Busto, R. et al. Progressive parenchymal deposition of β-amyloid precursor protein in rat brain following global cerebral ischemia. Acta Neuropathol 97, 359–368 (1999). https://doi.org/10.1007/s004010050999
Issue Date:
DOI: https://doi.org/10.1007/s004010050999