Abstract
We developed an HPLC method for analysis of the monosaccharide composition of fucoidans. The fucoidan was hydrolyzed into monosaccharides with 2 mol/L trifluoroacetic acid. Using ribose as the internal standard, the monosaccharide derivatives, obtained with 1-Phenyl-3-methyl-5-pyrazolone (PMP), were separated by reverse-phase HPLC using a gradient elution process, and monitored by ultraviolet detection at 245 nm. In the concentration range of 0.1–2.0 mmol/L, the peak area of each monosaccharide had a good linear relationship with its concentration (r 2>0.998). The average recoveries of mannose, rhamnose, glucuronic acid, glucose, galactose, xylose, and fucose were 86.2%, 95.1%, 62.5%, 102.0%, 94.8%, 66.6%, and 105.1%, respectively. This method was accurate and had good reproducibility and could be used to determine the monosaccharide contents of fucoidans.
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Supported by the Key Technology Research & Development Program of Shandong Province (No. 2007GG1005007)
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Zhang, J., Zhang, Q., Wang, J. et al. Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC. Chin. J. Ocean. Limnol. 27, 578–582 (2009). https://doi.org/10.1007/s00343-009-9205-0
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DOI: https://doi.org/10.1007/s00343-009-9205-0