Abstract
Two different methods for sugarbeet (Beta vulgaris L.) transformation were developed, one using Agrobacterium with excised cotyledons, the other, particle bombardment of embryogenic hypocotyl callus. Transformation efficiencies averaged 0.7% for the Agrobacterium method (number of transgenic plants obtained per treated cotyledon) and about 8% for the bombardment method (number of transgenic plants obtained per plate of embryogenic callus treated). Transgenic sugarbeet plants were produced carrying genes encoding either pathogen-defense-related proteins or the reporter enzyme β-glucuronidase (GUS) under transcriptional control of stress- or wound-inducible promoters. In addition, two plants were regenerated carrying a gene associated with enhanced insect resistance, the cytokinin biosynthesis gene, fused to a patatin gene promoter from potato. Expression of the GUS gene (gusA) under the control of the tobacco osmotin promoter was wound inducible with detectable activity at 8 h and maximal activity at 72 h post-wounding.
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Received: 11 August 1997 / Revision received: 2 July 1998 / Accepted: 22 December 1998
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Snyder, G., Ingersoll, J., Smigocki, A. et al. Introduction of pathogen defense genes and a cytokinin biosynthesis gene into sugarbeet (Beta vulgaris L.) by Agrobacterium or particle bombardment. Plant Cell Reports 18, 829–834 (1999). https://doi.org/10.1007/s002990050669
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DOI: https://doi.org/10.1007/s002990050669