Abstract
A hairy root clone (M8) of Atropa belladonna, producing high levels of tropane alkaloids, was established by transformation with Agrobacterium rhizogenes (MAFF 03-01724). Littorine, an intermediate of tropane alkaloids, was detected by high-performance liquid chromatography and gas chromatography-mass spectrometry in the alkaloid fraction of the hairy roots and identified by nuclear magnetic resonance analysis. Littorine was also detected in the non-transformed root culture of A. belladonna.
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Received: 18 March 1998 / Revision received: 15 June 1998 / Accepted: 3 August 1998
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Nakanishi, F., Sasaki, K. & Shimomura, K. Isolation and identification of littorine from hairy roots of Atropa belladonna. Plant Cell Reports 18, 249–251 (1998). https://doi.org/10.1007/s002990050565
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DOI: https://doi.org/10.1007/s002990050565