Abstract
Callus formation was achieved with root, hypocotyl, and cotyledon explants of niger (Guizotia abyssinica Cass.) cultivar Sahyadri on Murashige and Skoog medium containing 0.5 mg l–1 β-indoleacetic acid + 1.5 mg l–1 6-benzylaminopurine (BAP). Hypocotyl and cotyledon-derived calli when transferred onto a medium with 0.5 mg l–1 BAP produced an average of 12–32 shoots/ callus culture. The callus retained its potential for shoot regeneration for more than 19 months. The shoots formed an extensive root system and were transferred to pots kept in a greenhouse, where the survival rate was 98%. The plantlets flowered in vitro if transfer to fresh medium or to soil was delayed by 40–50 days. All regenerants were diploid with 2n=30.
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Received: 13 March 1997 / Revision received: 17 May 1997 / Accepted: 5 July 1997
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Nikam, T., Shitole, M. In vitro plant regeneration from callus of niger (Guizotia abyssinica Cass.) cv. Sahyadri. Plant Cell Reports 17, 155–158 (1997). https://doi.org/10.1007/s002990050370
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DOI: https://doi.org/10.1007/s002990050370