Abstract
Efficient and reproducible plant regeneration has been established from stem internode explants of Moricandia arvensis, a crucifer of special interest due to its C3-C4 intermediate photosynthetic activity. Somatic embryogenesis was induced in one-third of explants cultured on Murashige and Skoog based medium containing 9 mm 2,4-dichlorophenoxyacetic acid. High frequencies of plant regeneration (>90%) resulted when somatic embryos were germinated on medium lacking growth regulators. Regenerated plants were diploid, fertile and morphologically similar to seed-derived plants of M. arvensis. This is the first report of somatic embryogenesis in M. arvensis. This plant regeneration system should facilitate gene identification and localisation studies of C3-C4 physiology by insertional mutagenesis, a prerequisite for the isolation and transfer of genes involved in C3-C4 metabolism from Moricandia to cultivated brassicas.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 28 November 1996 / Revision received: 30 January 1997 / Accepted: 15 February 1997
Rights and permissions
About this article
Cite this article
Craig, W., Wiegand, A., O'Neill, C. et al. Somatic embryogenesis and plant regeneration from stem explants of Moricandia arvensis. Plant Cell Reports 17, 27–31 (1997). https://doi.org/10.1007/s002990050346
Issue Date:
DOI: https://doi.org/10.1007/s002990050346