Abstract.
The cloning of fga1, the gene encoding a G protein α subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino acid residues) showed high identity to those of G protein αi family proteins from other filamentous fungi. Disruption of fga1 had no effect on vegetative growth, but reduced the conidiation and pathogenicity of the fungus. Disruptants also showed a decreased level of intracellular cAMP and increased resistance to heat shock at 45 °C. These results suggest that the Gα subunit encoded by fga1 is involved in a signal transduction pathway in F. oxysporum that controls conidiation, heat resistance and pathogenicity.
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Jain, S., Akiyama, K., Mae, K. et al. Targeted disruption of a G protein α subunit gene results in reduced pathogenicity in Fusarium oxysporum. Curr Genet 41, 407–413 (2002). https://doi.org/10.1007/s00294-002-0322-y
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DOI: https://doi.org/10.1007/s00294-002-0322-y