Abstract
High yields (1939 U/ml) of an alkaline protease were obtained in batch fermentation of a Bacillus sp. using a response surface methodology. The interaction of four variables, viz., starch, peptone, incubation time, and inoculum density, suggested inoculum density to be an insignificant variable. However, incubation time had a profound effect on protease yields at all the concentrations of carbon and nitrogen used. The response surface raised and flattened with increase in time of incubation, and maximum protease production up to 1939 U/ml was obtained after 96 h of incubation. The model equation obtained was validated experimentally at maximum starch (15 mg/ml) and peptone (7.5 mg/ml) concentration with increased incubation time up to 144 h in the presence of minimum inoculum density (1%). An overall 2.6-fold increase in protease production was obtained as compared with mean observed response (750 U/ml) at zero level of all variables.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Author information
Authors and Affiliations
Additional information
Received: 19 July 2001 / Accepted: 15 August 2001
Rights and permissions
About this article
Cite this article
Puri, S., Beg, Q. & Gupta, R. Optimization of Alkaline Protease Production from Bacillus sp. by Response Surface Methodology. Curr Microbiol 44, 286–290 (2002). https://doi.org/10.1007/s00284-001-0006-8
Issue Date:
DOI: https://doi.org/10.1007/s00284-001-0006-8